We narrowed to 167 results for: 11

1. CRISPR Methods for Bacteria: Genome Engineering, CRISPRa, CRISPRi, Base Editing, and More

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   Blog Post

   ...Mycobacterial species, Sarah Fortune’s lab screened 11 other Cas9 orthologs to identify a more efficient... Phage-Delivered CRISPR-Cas3 Antimicrobials. mBio 11: . https://doi.org/10.1128/mbio.00019-20 Singh AK...

2. Optogenetics + CRISPR, Using Light to Control Genome Editing

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   Blog Post

   ...expression of human IL1RN, the researchers saw an 11-fold increase in mRNA levels after 2 hr and a 400...control of endogenous gene activation. Nat Chem Biol 11:198–200 . https://doi.org/10.1038/nchembio.1753 Richter...

3. Delivery Methods for Generating iPSCs

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   Blog Post

   ...generating iPSCs by plasmid transfection where 2 of 11 clones tested had plasmid integration. Another challenge...Nonstructural C Protein and Endogenous MicroRNAs." Plos One 11, no. 10 (2016). PubMed PMID: 27764162. PubMed Central...

4. Arf GTPase Family

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   Collection

   ...and challenges. Molecular Biology of the Cell , 30 (11), 1249–1271. PMID: 31084567 (Link opens in a new ...

5. CRISPR Plasmids - Bacteria

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   ...using CRISPR. Biochem Biophys Res Commun. 2013 Aug 11. pii: S0006-291X(13)01329-6. doi: 10.1016/j.bbrc.2013.08.013...

6. 22 Hot Plasmid Technologies from 2014

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   Blog Post

   ...12005-15. Hernandez et al., Autophagy 2013 Nov 1;9(11):1852-61. A quicker method for protein inactivation...variants. Klapoetke et al., Nat Methods 2014 Mar 11;(3):338-46. For more information and descriptions....3179. Martell et al., Nat Biotechnol 2012 Nov;30(11):1143-8.  A new tool for CRISPR gRNA validation...

7. Viral Vectors 101: Systemic Capsids

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   Blog Post

   ...non-human primate brain. Nature Nanotechnology, 1–11. https://doi.org/10.1038/s41565-023-01419-x Deverman...cellular receptor for AAV-PHP.B capsids. PloS One, 14(11), e0225206. https://doi.org/10.1371/journal.pone.0225206...prioritization for cell type-specific enhancer design. eLife,11, e69571. https://doi.org/10.7554/eLife.69571 Liguore...Primates Compared with Mice. Molecular Therapy, 27(11), 2018–2037. https://doi.org/10.1016/j.ymthe.2019.07.017...

8. Viral Vectors 101: Inverted Terminal Repeats

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   Blog Post

   ...Zhou et al., 2017). The most common variant is an 11 bp deletion in the B or C domain of one ITR. These...

9. Summer SciComm Series: Cognitive Load

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   Blog Post

   ...-page genre fiction novella as it does to read an 11-page Cell paper - and sometimes, it might actually...

10. BeHeard Award 2018: Diseases of Glycosylation, Arginine Mutagenesis, & Neural Development

    Type

    Blog Post

    ...other labs discovered that the in vitro functions of 11 different Arg-mutant enzymes could be rescued by ...

11. GPCRs: How Do They Work and How Do We Study Them?

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    Blog Post

    ...subtypes, based on their Gα subunit: Gαs, Gαi/o, Gαq/11, and Gα12/13. Gβ and Gγ are closely associated and...

12. Pairing CombiGEM and CRISPR for Combinatorial Genetic Screening

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    Blog Post

    ...PMID: 26280411. PubMed Central PMCID: PMC4785103. 11. Wong, Alan SL, et al. "Multiplexed barcoded CRISPR-Cas9...

13. Enhancing CRISPR Targeting Specificity with eSpCas9, SpCas9-HF1, & HypaCas9

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    Blog Post

    ...EMX1 locus in human embryonic kidney (HEK) cells, 11 of these initial mutants retained the on-target activity...

14. Plasmids 101: Golden Gate Cloning

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    ...Engler C, Kandzia R, Marillonnet S. PLoS One 2008;3(11):e3647. doi: 10.1371/journal.pone.0003647. Epub 2008...

15. Protocol - How to Run an Agarose Gel

    Type

    Protocol

    ... you want to load 1.0 μg in 10 μL, make 1.1 μg in 11 μL. Reference Page Top Index...

16. Nanoblades: Tiny CRISPR Ninjas for Genome Editing Difficult Cells

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    Blog Post

    ...of the chimeric and fully albino mice ranging from 11% to 100%, a level similar to other approaches.  Nanoblades...

17. Pathways Over Time Plasmids Engage Students in Functional Genomics Research

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    Blog Post

    ...Dujon, B. Yeast evolutionary genomics. Nat Rev Genet 11, 512-524. (2010). PubMed PMID: 20559329. 8. Reeves...

18. Virus Protocol - Generating Stable Cell Lines

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    Protocol

    ...Sanjana NE, Shalem O, Zhang F. Nature Methods. 2014 Aug;11(8):783-4 (Link opens in a new window) . Last reviewed...

19. Plasmids 101: Degron Tags

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    Blog Post

    ...via a self-excising degron. Nat Chem Biol. 2015 Sep;11(9):713-20. doi: 10.1038/nchembio Nabet B, Roberts...

20. Adenoviral Delivery of CRISPR/Cas9 Aims to Expand Genome Editing to Primary Cells

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    Blog Post

    ...genome editing with engineered nucleases. Nat Methods 11:1051–1057 . https://doi.org/10.1038/nmeth.3075 Lukashev...