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Learn more about non-homologous end joining (NHEJ) and how it relates to genome engineering technologies such as CRISPR/Cas9.

CRISPR Cas9 gRNA plasmids from the Zhang lab

This post discusses takeaways from, and tools presented at, the Deaminet 2026 conference, focusing on base editors, prime editors, and translational work.

Designing a CRISPR experiment in bacteira? Check out these tools for genome engineering, CRISPRa, CRISPRi, base editing, and microbiome engineering.

The tools of optogenetics can be combined with CRISPR to generate inducible genome edits and gene regulation systems.

Learn about the many ways you can deliver reprogramming factors to generate induced pluripotent stem cells (IPSCs).

Standard protocol for performing agarose gel electrophoresis, including tips to improve resolution and separation of bands.

22 of the interesting and popular hot plasmid technologies of 2014 from Addgene's plasmid repository.

Curious about systemic capsids? Looking for one to test in your experimental system? This 101 is an introduction to systemic capsids uses and types!

Browse Addgene's collection of ARF family plasmids. ARF family members include regulatory GTPases, GEFs, and GAPs.

Browse AAV transfer, packaging, and helper plasmids in Addgene's repository.

Sequence and ligation independent cloning (SLIC) lacks the strict requirements of many standard cloning techniques. Learn how to use SLIC in this post.

Curious about what an ITR is and why it's so important for an AAV? Learn all about inverted terminal repeats in this Viral Vectors 101 post.

What are we asking of our audience when we tell them about our science? Cognitive load explains how your learners have to work too.

GPCRs are receptors that respond to stimuli and elicit cellular responses. Learn about the parts of GPCRs, how they work, and tools to study them.

Congratulations to Paula Videira, Michael McMurray, & Richard Gronostajski who each are being awarded free plasmids form Addgene. Read on to learn how these researchers plan to use plasmids for research on diseases of glycosylation, arginine mutagenesis, & neural development.

The CombiGEM technology allows a researcher to design and screen CRISPR gRNA libraries for effects on your biological phenomenon of choice.

Rationally engineered Cas9 variants eSpCas9 SpCas9-HF1 enhance CRISPR on-target specificity. Learn how in this post.

Golden gate cloning can be used to efficiently and inexpensively clone multiple inserts into a single vector. Learn the basics of golden gate here.

Protocol to generate stable cell lines expressing a gene of interest from an integrated lentiviral vector.