We narrowed to 391 results for: ARIA;

...technology itself is constantly improving. Cas9 variants have been modified for genome editing, activating...specificity of the Cas9 nuclease with engineered variants: eSpCas9, SpCas9-HF1, & HypaCas9. The off-target...off-target effects” have included using a Cas9 nickase variant, lowering Cas9 expression, and truncating gRNA ...Berkeley recently added their own hyper accurate Cas9 variant (HypaCas9) to the pool of enhanced CRISPR tools...HypaCas9 (SpCas9 N692A/M694A/Q695A/H698A). This new variant displays comparable on-target activity and similar...

...Over 75,000 pathogenic genetic variants have been identified in humans and cataloged in the ClinVar database... potential to correct only a minority of those variants in most cell types. But prime editing, a CRISPR...research, the Liu lab created and evaluated 19 PE1 variants with RT mutations known to increase activity, ...PE2max, PE3max, etc. PE6: Specialized prime editing variants Four years after their initial publication, the...from PE6e-g produced additive improvements. These variants are worth testing if you want to make your prime... Figure 2: Decision tree for selecting a PE6 variant. PE = prime editor; RTT = reverse transcriptase...

...COVID-19 research in other labs. Spike protein, variants, and pseudotyping The SARS-CoV-2 virus depends...cellular receptor. To more easily study the spike variants, scientists have developed ways to pseudotype ... has had many opportunities to evolve. Several variants are now circulating and plasmids containing these...pseudovirus and those encoding different spike protein variants that can be incorporated into pseudoviruses. The...at neutralizing the different SARS-CoV-2 spike variants. You can learn more about their research in this...

...modified ABE7.10 accordingly which resulted in variants named “ABE8.x”: “8” stands for the eighth round...x-d.  This resulted altogether in 40 new ABE8 variants. When comparing on-target DNA editing efficiency...replaced S. pyogenes Cas9 in the ABE complex with PAM-variant Cas9 proteins: the S. pyogenes NG-Cas9 (PAM: NG...PAM: NNGRRT) to create Sa-ABE8.x-m/d. With these variants, ABE8 could target many additional locations in... base editor: ABE8e Read about xCas9, a CRISPR variant with PAM flexibility Resources on Addgene.org ...

...either transcript variant led to robust and specific upregulation of the targeted variants without inducing...activation? To summarize, CRISPRa uses a nuclease-dead variant of Cas9 (dCas9) and engineered combinations of ...inducing expression of the non-targeted variant. Moreover, isoform-specific expression of Bdnf resulted in... between the Bdnf I and Bdnf IV overexpression variants were other immediate-early genes associated with...

...utility of the system for use with low affinity variants of TEV. The new system has been demonstrated to...the evolution platform to generate TEV protease variants. Catalysis by TEV protease allows a transcription...transgene design and delivery. To overcome these variations in human pluripotent stem cells(hPSCs), Lindy...fluorescence protein-based calcium sensors (jYCaMP1), variants of the calcium indicator jGCaMP7, that are optimized...new calcium sensors include syn-jYCaMP1s (slow variant), the Cre-dependent syn-FLEX-jYCaMP1s, and the ...

...pH-insensitive RFP variant, DsRed.T3, that’s connected to a pH-sensitive GFP variant, super ecliptic pHluorin...fixed cells. Find the mitochondria-targeted Keima variant at Addgene! mRFP-GFP-LC3 tandem fluorescent protein...at Addgene! References Klionsky, Daniel J., Ana Maria Cuervo, and Per O. Seglen. "Methods for monitoring...

...impractical to catalog every variant of a feature. Instead, we identified common variants, and then crafted a ...threshold. This issue is addressed by adding more variants to the database. Another limitation is that by...augment the database with new fluorescent protein variants. The future of DNA feature identification What...

...the best advice is to try a couple of promising variants to check how they perform under your experimental... a fluorescent protein (fusion) there is huge variation in the fluorescence intensities of individual ... plasmid uptake by cells. To correct for this variation, a reference fluorescent protein that is expressed... from Addgene. Another way to dodge cellular variation observed in mammalian cells is to tag an endogenous...

...uncover new gene variants associated with conditions of interest, then studying those variants in model systems...implicated in disease or, in cases where particular variants or mutations have been reported in people, we .... You could use the kit to insert all of those variants into stem cells with the same genetic background...

...within cell populations and over time resulting in variable gene expression that can impact observed phenotypes...overrepresented compared to those further away. As a result, variable gene expression can make data hard to interpret... in the same background resulted in a 20-fold variation of gene expression. Because pSC101 is not representative... complex, stabilized promoters can eliminate variability between the different components. When your pathway...

...relatively pH-insensitive RFP variant, DsRed.T3 and a pH-sensitive GFP variant, super ecliptic pHluorin (...sequence. Under normal growth conditions, both variants are excluded from the vacuole and emit red and...the cytosol-targeted and mitochondria-targeted variants are available from Addgene. References 1. Rosado...

...Cas protein variants may also be useful for targeting ncRNAs. For example, the Cas12a variant can cleave...genomic elements (Hazan and Bester 2021). Other Cas variants Another option is CRISPR/Cas13, which targets ...specifically edit or degrade targeted lncRNAs. The Cas14 variant is a smaller and more versatile option. It can ...

...expressing variants of Yellow Fluorescent Protein (YFP). The team identified a new YFP variant, mGold. mGold...Birsoy. Find the library here A new SaCas9 ABE variant (microABEI744) has improved on-target editing efficiency...compared to current N-terminal linked SaCas9 ABE variants. It's also one of the smallest AAV-deliverable...

...recombinant antibody. Researchers first sequenced the variable domains of Myc1-9E10 in the late 1990s (Fuchs,...anti-Myc [9E10] Fab fragments and single-chain variable fragments.  Abi, on the other hand, might...mentioned above basically involve taking the Myc1-9E10 variable regions and sticking them onto different scaffolds...Myc tag monoclonal antibody 9E10 displays highly variable epitope recognition dependent on neighboring sequence...

... and this nuclease-null or “dead” Cas9 (dCas9) variant can then be fused to effector domains such as transcriptional...limitations to a system that relies on two distinct Cas9 variants—nuclease-competent or nuclease-null—in order to...with both the nuclease-positive and nuclease-null variants of Cas9 along with the necessary gRNAs, but it...control which gRNA becomes complexed with which variant of Cas9. To circumvent this problem, previous studies...

...such as malaria and dengue for many years. Since more than a thousand children died of malaria today, ... tropical mosquito vectors of diseases such as malaria and dengue are currently being performed in regions... cas9 gene). An immunizing reversal drive is a variant that also spreads through the wild population and...

...FPs just keeps getting larger with hundreds of variants available. Read on to familiarize yourself with...emission wavelenght range).  By mutating GFP, the variants blue FP (BFP), cyan FP (CFP), and yellow FP (YFP...YFP) were derived. For a breakdown of GFP, it's variants, and their relevant mutations, check out Marcy's...with very comprehensive data sheets on many FP variants Plasmids 101: Green Fluorescent Protein (GFP) ...

... Having incorporated a discovery-based lab on planarian regeneration into one of my own courses, I was...required for stem and progenitor cell maintenance in planarians in Developmental Biology (Kimball et al., 2020...undergraduates conducting NIH- and NSF-funded research on planarian flatworms both in and out of the classroom.   ...required for stem and progenitor cell maintenance in planarians. Developmental Biology. 457:119-127. Knutson,...

... et al., 2016). For TREE they engineered a BFP variant that undergoes conversion to GFP after being targeted...changes the histidine to a tyrosine generating a GFP variant (GFPY66) that has a shifted emission spectra. Thus...the test by infecting HEK-293 cells with the BFP variant, a BE4am-Cas9 base editor, and sgRNA vectors for...the Wang and Brafman lab uses an engineered BFP variant that converts to GFP after a base editing event...