... basic research, there is resistance to change, public support is weak, government support is “feckless...be radically overhauled.  Finally, the science publishing system needs to be changed.  Dr. Bourne charged...applause when he talked about how he boycotts publishing in all but open access journals.  Dr. David Glass...astounding lack of reproducibility being recognized in published work even in the “best” journals.  He pushed for...

...decision remains spread across literature in publications that introduce these tools. Years ago, Kurt...made to moderate and cross-check all data with published literature as soon as possible after submission... queries.  Looking for a bright, monomeric FP published in the last few years with an emission peak around...collection pages can be kept private, or shared publicly (so feel free to moderate your own version of ...

...why the results you've measured should change established theory. So, what can you do to ensure that your...as they do science outreach and engage with the public, and learn from the process of writing papers and...scientists need to communicate with non-scientists, to establish broader impacts for their work in education and...before you complete your work. If you’re giving a public talk, ask a friend who works in a different field...

...Raspberry Pi, the Arduino, or 3D printers are key to enabling this work. Sharing open-source plans that rely...worldwide. Distributing hardware via globally established makerspace networks and training users via workshops...studying plankton using flow cells, has become an established alternative to high-priced commercial devices...developed techniques are the key enablers for making assemblies open-source. The sharing of digital representations...

...Hijacking tRNA processing to make gRNAs In a recently published paper, Yinong Yang’s group did just that as part... Mar 2. PubMed. Find the plasmids from this publication at Addgene Other Techniques for gRNA Multiplexing...May 15. PubMed.  Find the plasmids from this publication at Addgene Dimeric CRISPR RNA-guided FokI...Apr 25. PubMed.. Find the plasmids from this publication at Addgene ...

...involved in plant synthetic biology. Challenge #1: Public perception of synthetic biology One of the biggest...difficulties that plant biotechnologists face is public perception of genetic modification (GM) and synthetic...benefits and future potential of the technology to the public in an open, responsible manner. At Revolution Bio...obstacles to plant synthetic biology In addition to public resistance, an overall limited understanding of...

...As evidenced by all the CRISPR publications, press, and plasmids out there, it’s obvious that CRISPR ...PubMed PMID: 26628643. Find plasmids from this publication at Addgene. 2. Nishimasu, Hiroshi, et al. "Crystal...PubMed PMID: 26735016. Find plasmids from this publication at Addgene. 5. Chen, JS, et al. "Enhanced proofreading...PubMed PMID: 28931002. Find plasmids from this publication at Addgene. Resources on the Addgene Blog Read...

... members (like me!) are dedicated to creating a public and accessible database for low-cost, high-quality...positive, it will be released through the website for public reference. The Gift of Giving As an open-source...for goldilocks antibodies and, alongside that, a publicly available 2D/3D mouse atlas from antibodies that...resolving the antibody crisis. The mAb3D Atlas is a public service to the science community, and I am excited...

...insight into an area - not just working within the established network of your peers. Furthermore, using new...non-scientists. Most fellowships are funded by the general public (either through taxes or charitable donations) ...of the work was. Ideally this would be results published in a paper, but positive outcomes can include ...! Seán was a PhD student at University College Dublin & a post-doc at Cambridge University where he studied...

...Institute (Harvard University), we developed two new enabling technologies for the field of synthetic biology... journal Cell just six weeks later. The Zika publication timeline I think we can speak for all the authors...that can take pathogen sequence information from public databases to the production of validated sensors...PubMed PMID: 27160350. Find Plasmids from the publication at Addgene 2. Green, Alexander A., et al. "Toehold...

...to dimerization (i.e. EGFP) [1, 2] or even form obligate tetramers (i.e. DsRed) [3]. This is a problem ...and difficult to ignore [1, 2]. 2. Fusion to an obligate dimer or oligomer Several cell proteins normally...even if they were described as monomeric in a publication. The gold standard in assays used to determine...moderately dimerizing FPs such as EGFP, but not for obligate oligomers such as DsRed or high affinity FPs such...

...highlight some of the reagents that have been made publicly available through Addgene.  What makes CRISPR... Individual gRNAs are designed in silico using publicly available gRNA design software and synthesized...derivatives of Cas9. Several CRISPR libraries are publicly available through Addgene, with more being added...information on CRISPR screens check out Addgene’s 2016 publication “Practical Considerations for Using Pooled Lentiviral...

... far beyond the laboratory and seriously damage public trust in scientists, experiments involving potential...negligible level. One major reason we chose to publish the concept and likely capabilities of RNA-guided...biology and genetics because the underlying advance enabling RNA-guided gene drives is Cas9 genome editing....about gene drives can be found in the referenced publications. Thank you to our guest blogger! An inventor...

...autosomal recessive, severe, childhood-onset form of blindness name Leber’s Congenital Amaurosis (LCA). Shortly...disease that results in combined deafness and blindness) is caused by defects in the gene MYO7a, at ~7...further reading 1. FDA approves gene therapy for blindness 2. Gene Therapy emerges from disgrace to be the...restores vision in a canine model of childhood blindness. Nature Genetics 28, 92-95. PubMed PMID: 11326284...

...papers. Maybe they started their deposit prior to publication and were able to include specific plasmid ID ... antibody plasmids from the James Trimmer Lab published in eLife. Addgene is currently distributing 94...terms we can use to find materials from this publication. Searching for the title of a paper works You...filters Not every plasmid search will start from a publication. You may have an idea for a plasmid that would...

...PubMed PMID: 26422227. Find plasmids from this publication at Addgene. 2. Zetsche, Bernd, et al. "Multiplex...PubMed PMID: 27918548. Find plasmids from this publication at Addgene. 3. Kim, Daesik, et al. Genome-wide...PubMed PMID: 27272384 Find plasmids from this publication at Addgene. Kleinstiver, Benjamin P., et al....PubMed PMID: 27347757. Find plasmids from this publication at Addgene. Makarova, Kira S., et al. "An updated...

...path.  A part of the career path story for most established grant professionals is learning to succeed in... with clear budget narratives, logic models, established partnerships and collaborations, and commitments...grants profession? The number of organizations, both public and private, that qualify for grants, the amount... panels and to review education materials for publishers. When the community orchestra needed a grant ...

...sequence assembly of the zebrafish genome was published in 2013, revealing that about 70% of human genes...for generating transgenic zebrafish are well established (Felker and Mosimann, 2016). One common and robust...zygote transforms into an organism with a clearly established body plan including head, eyes, and tail. Compared...different methods have been developed, relying on established reagents such as CRISPR/Cas9, Tol2, and the high-quality...

...Principal Investigator meeting of the NSF EDGE (Enabling Discovery Through Genomic Tools) Program and I... encouraged to not just wait for papers to be published, but to engage in ongoing, real-time dissemination...dissemination of information and materials. Many groups publicized workshops on their organismal systems for scientists...A paper on stickleback fish science was first published in 1867. It said they were hard to raise because...

...have the same blind spots, and there wouldn’t be anyone to question these blind spots. Blind spots often...result of our diversity which in turn minimizes the blind spots in our work.  Agile at Addgene We follow an...