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...Blogs CasPEDIA Content last reviewed: 9 December 2025 Do you have suggestions for other plasmids that should...

...Blogs CasPEDIA Content last reviewed: 9 December 2025 Do you have suggestions for other plasmids that should...

...they do it.    Finding Conflict in Science  In all science communication, what you are doing is telling...they’re firmly part of the story to the scientists doing the work.  And while these pieces aren't always ...of my story” and that’s a very valid critique! But do you remember earlier when I said that even when we...t that sense of curiosity and wonder why you’re doing and sharing science in the first place?   References...

...that we are doing just that. Do you like the direction the blog has taken over the past year? Do you think... 7. Career Coaching for Scientists: Why and Where Do I Find One? If you need help making a transition...Addgenie Joanne Kamens explains what career coaches do and offers up some career coach suggestions. 8. ...

...to the principle that you can't motivate people to do things, you can only demotivate them. The primary...less a really good one). While getting your PhD or doing a postdoc few science trainees will have opportunities...hard and most people have some learning they need to do before they can be good at it. Like any other skill...freedom and diversity built into their jobs Chance to do/learn new things, diversity of responsibilities, ...if opportunities for learning and personal growth do not exist or if they feel underutilized in their ...

...customer place an Addgene order using a PO number? Why do I have to order directly from your website? Many ...sent to the contact email addresses provided. How do I place an order using a PO number? Placing an order... PO, learn more about how to create a quote. How do I pay the invoice? After your Addgene order ships...this article, check out our Help Center article How do I pay by purchase order (PO) number? You can find...

... news and updates. Scientists must do science to be happy. What do we have to change to ensure that all...all scientists can have successful careers doing science in some form? There is a lot of talk about the... behind and some just don't have the resources to do enough. There are still university research departments...

...researchers in the field. Dos and don’ts when publishing a scientific manuscript Dos: Make sure your paper...editor or reviewer over their decision. If you do object, do so in a respectful and professional manner. ...need to include in your manuscript. What information do I need to include in my paper? More and more journals...

...you in building a grant career. Do you have a passion for the arts? Do you find satisfaction in helping...Chat, and then our grant training and mentoring. How do I become a grant professional? Employment opportunities...healthcare facilities and with private businesses. How do you get the expertise needed to enter the grants ...helping your local food shelf raise money? Do you have experience with research grants? Are you interested...find work and target your professional development. Do you want to work in a particular area, e.g. education... childhood development, or community development? Do you want to focus on a part of the grant development...

...Blogs CasPEDIA Content last reviewed: 17 October 2025 Do you have suggestions for other plasmids that should...

...Wood of Duke University Medical Center. “While we do not yet have a comprehensive understanding of how... become resistant, an emerging theme is that they do so by activating signaling pathways controlling properties...contexts, so he assembled a plasmid kit designed to do exactly that. As described in the journal Science... world who are interested to use our libraries to do things - sometimes drug screens and sometimes totally...

...Blogs CasPEDIA Content last reviewed: 17 October 2025 Do you have suggestions for other plasmids that should...

...Blogs CasPEDIA Content last reviewed: 9 December 2025 Do you have suggestions for other plasmids that should...

...are trying to achieve: Do you want to permanently knock out or knock-in a gene? Do you want to activate ...Are you trying to create a single point mutation? Do you want to create a fusion with a reporter protein...appropriate expression system and delivery method: Do you need stable integration or is transient expression...sufficient? Which cell types will you be editing? Do you want to deliver the components as DNA, or would...surprised at the number of options available - how do you choose between them? One of the first steps is...

... what position do you want your gene to be in relation to the rest of the genes? And do you want it to...the Modular Cloning (MoClo) system and what it can do for you, and you’ve chosen one of the MoClo kits ...coding sequence, a terminator, or other plasmid part? Do you want to fuse your protein to any tags or fluorescent...your MoClo assemblies in silico before you start doing your cloning. Most sequence analysis software includes...preventing your overhangs from aligning properly. Do this sequentially for your Level 0, Level 1, and ...viability. When handling your MoClo plate, always do so in cold conditions and preferably on dry ice. ...

...have any questions, comments or suggestions, please do not hesitate to email me at [email protected] need a Nature/Cell/Science paper. You do need multiple first/corresponding author publications...exciting idea in a swamp of unnecessary details. You DO NOT need to present a detailed review of your field...field or the finer points of your techniques. You DO need to be prepared to know the literature of your...proteins is essential for human health. Failure to do so is implicated in several human diseases. Proper...bring in grants. What kind of colleague will you be? Do you understand what it takes to be successful in ...a Department's/Institution's success is the task. Do your homework. Learn what your future potential colleagues...

...the Addgene Blog AAV Titers: Where do they come from and what do they mean? AAV Vector Quality Control...AAV Protocol (Step 10) for instructions on how to do this. Concentrating your virus will allow you to ...titer when looking for effective gene expression. How do you achieve high titers in the first place? Some ...surrounding solution to ensure excess viral particles do not remain adhered to the membrane. Be careful when...

... is done within publication years. What do we find once we do this adjustment? That papers with plasmids...story had us wondering: is there a larger trend here? Do papers associated with Addgene deposits accumulate... with Addgene plasmids get more citations So what do we find? Lots more citations for the papers with ...analysis cannot rule out all other explanations, we do think that it lends weight to the argument that Addgene...

...replicating previous research findings so difficult to do?  There are four reasons that research can be difficult..., QRPs, and small samples, then when replications do not come out the same as an original research finding...difference for the robustness of the findings.   How do we make research more reproducible? Combat unclear...of other researchers (or your own from years ago!) do you really appreciate how few details are provided...while the research is ongoing, but all registries do require that research plans become public eventually...

...content, website, Data Hub, and everything else we do! The goal of Addgene’s educational content Addgene...their favorite science topics. What better space to do that than in the Addgene blog?  Fig. 1: One...editing process and final check before publication.   Do research! Nearly everything we write entails a primary...papers or image sharing repositories. Then we format, do a final review to catch those pesky typos, and hit...advance the scientific community, and we can only do that with your help.     Additional Resources on...