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We narrowed to 638 results for: Erf

Showing: 81 - 100 of 638 results

  1. Rett Syndrome

    Type
    Collection
    ...Models Induced pluripotent stem cells (iPSCs) are a powerful system for generating Rett syndrome-specific differentiated...

  3. Protocol - How to Ligate Plasmid DNA

    Type
    Protocol
    ...fragments. This reaction, called ligation, is performed by the T4 DNA ligase enzyme. The DNA ligase catalyzes...digested vector backbone with a phosphatase before performing the ligation reaction (phosphatase removes the...

  4. Deisseroth INTRSECT Collection

    Type
    Collection
    ...Implementation Plasmids References The development of powerful molecular reagents for biology have propelled ...

  5. Retrograde AAV viral preps

    Type
    Collection
    ... vivo testing of these viral preparations was performed by the Karpova lab. Results for our quality control...

  6. Luciferase Plasmid Collection

    Type
    Collection
    ...Mikko Taipale 12563 PCD FL0X Firefly Insertion of imperfect miRNA sequence in the 3' UTR Carl Novina 33154...

  7. Caltech Systemic Capsids

    Type
    Collection
    ...Addgene's blog on Systemic Capsids . To review performance data of each of these serotypes, please refer...

  8. Protocol - How to Run an Agarose Gel

    Type
    Protocol
    ...Standard protocol for performing agarose gel electrophoresis, including tips to improve resolution and...Watch the protocol video below to learn how to perform gel electrophoresis. Equipment Casting tray Well...

  10. Zhang Lab CRISPR Page

    Type
    Collection
    ...lentiviral CRISPR libraries (both human and mouse) for performing genome-wide knockout screens. The libraries are...

  11. Tetracycline Inducible Expression

    Type
    Collection
    ...Plasmids Inducible and repressible promoters are powerful tools to control gene expression and study biological...

  12. Educational Planarian Tools for Teaching Developmental Biology Techniques

    Type
    Blog Post
    ...function by RNA interference (RNAi) RNA interference (RNAi) is a powerful tool for performing targeted knockdowns...the students performing these experiments have a good baseline for comparison. Performing WISH using these...are too few resources to teach students how to perform these experiments in the beginning. In most classrooms...observation and dissections. Students don’t usually perform more modern techniques used in genetics or molecular...addition, the Sánchez lab has made it easier to perform advanced developmental biology experiments in planarians...genes. Double-stranded RNA (dsRNA)-mediated RNA interference (RNAi) to knockdown expression of specific gene...both WISH and RNAi experiments, as well as how to perform worm amputations. They also created an online companion...

  13. Deep Dive: Statistical Tests (Comparisons)

    Type
    Blog Post
    ...than one another, a two-tailed test should be performed. This test will assess if the mean of an experimental...tests, referred to as post-hoc analysis, must be performed. These will be discussed later. One and two-way...within your ANOVA/ANCOVA analysis. If you have performed a MANOVA/MANCOVA and want this information, multiple...multiple ANOVAs/ANCOVASs must be performed first, and then post hoc tests can be run on these tests. ...can reduce the family-wise error rate by only performing the comparisons you really care about. Family-wise... positive occurring when many comparisons are performed. For example, if your alpha is set at .05, then...test will be incorrect. If you have 5 samples and perform every pairwise comparison (10 in total), your likelihood...

  14. Antibodies 101: Introduction to Immunofluorescence

    Type
    Blog Post
    ...optimization as each antibody performs differently and some antibodies impact the performance of other antibodies...beautiful results. Performing an immunofluorescence experiment The process to perform IF can be broadly...important considerations to make when planning and performing an IF experiment. What is immunofluorescence... different species and colors makes it easy to perform multiplexed IF. By using multiple primary antibodies...in IF experiments. Precisely which controls you perform will depend on your experimental context.  Choosing... to use, then the primary antibody will likely perform as expected in both.  How do you find validated...may be, it is always important to verify their performance in your hands and in your specific experiment...

  15. New Optimized Genome-wide CRISPRko, CRISPRi, and CRISPRa Libraries

    Type
    Blog Post
    ...pooled libraries have allowed scientists to easily perform genome-wide screens to effectively and efficiently...human genome-wide CRISPRko (knock out), CRISPRi (interference), and CRISRPa (activation) libraries that do...that improved sgRNA activity and thus library performance. The Root and Doench lab’s optimized genome-wide...CRISPRi, and CRISPRa libraries have been shown to outperform other popular CRISPR libraries (see below). Let...colon cancer) cells and assessed the libraries performance via the depletion of a gold standard set of genes...developed sgRNA-level metrics to assess library performance in viability screens. Based on these metrics ...sgRNAs, Brunello even with only a single sgRNA outperformed another CRISPR library with 6 sgRNAS, highlighting...

  16. Viral Production at Addgene

    Type
    Blog Post
    ...protocols and performing rigorous quality control (QC). The specific QC experiments performed varies for ...of Pennsylvania Vector Core. Transfections are performed using the transfer plasmid, a plasmid encoding...and stored at -80 °C. Titer Titering is either performed by Addgene or by the University of Pennsylvania...Pennsylvania Vector Core. In general, titering is performed by the facility that produced the viral vector lot....Pennsylvania Vector Core is (as of April 2016) also performed by ddPCR.  Quality Control Addgene ensures high.... To learn which specific QC experiments were performed on your lot, please contact us. Full sequencing...the Viral Genome Next-generation sequencing is performed on viral genomes isolated from the final AAV preparation...

  17. Antibody Validation for Flow Cytometry

    Type
    Blog Post
    ...is, an antibody shown to perform well in one set of conditions may not perform as well in other conditions...approach is RNA interference (RNAi). The most common method is transfection of short interfering RNA (siRNA...assumed (Ayoubi et al., 2023). Additionally, their performance depends on the specific protocol used and the...For example, we have found that some antibodies perform well in a PFA-saponin fixation and permeabilization...population (Figure 1), further optimization can be performed to both maximize the separation and reduce any... is possible to design experiments where the performance of the antibody can be assessed through its ability...different conjugates. Summary Flow cytometry is a powerful tool to delineate cell populations and to study...

  18. Antibodies 101: Validation

    Type
    Blog Post
    ...your antibody performs as expected in one application does not mean that it will perform as expected in...doesn’t necessarily mean that you yourself have to perform extensive validation experiments for every antibody...data, you may need to roll up your sleeves and perform some extra validation experiments yourself.  Goal...researchers can use to show that their antibody performs as expected. One of the benefits of these pillars...levels tend to correspond to protein levels, so you perform immunohistochemistry with your antibody on these... the epitope recognized by your antibody. You perform immunocytochemistry on these mutant cells as well...caveat is that different antibodies may or may not perform well under the same conditions. Example: You want...

  19. Simplify Cloning with in vivo Assembly

    Type
    Blog Post
    ...homemade competent cells of 107 CFU/μg are perfectly fine, yet performing multiple modifications will require... pathway to allow any cloning procedure to be performed using a simple two-step, 2 hr protocol prior to...adopted by any laboratory. All procedures are performed using the same three step protocol:  Fragment...efficiency of the strain: when asking the cell to perform multiple recombination events, all linear fragments... design (Figure 2), IVA cloning can be used to perform any plasmid modification, from insertions, deletions... reverse primer. Mutagenesis - Mutagenesis is performed by designing primers astride the old codon, amplifying...primers can be used in combination to simultaneously perform modifications, simply by adding all the necessary...

  20. CRISPR 101: Off-Target Effects

    Type
    Blog Post
    ... line to perform downstream characterization of, isolating 2 or 3 distinct clones to perform key experiments...CRISPR-Cas system localizes to unintended sites and performs its programmed function there, this is an off-...off-target effects, but it’s important to not let ‘perfection’ get in the way of ‘good enough’. Predicting ...would confound your experimental results and 2) perform targeted off-target measurements at those sites... to quantify off-target effects entirely is to perform whole genome sequencing. While this may be unnecessary... The con to these approaches is that NHEJ must perform the repair to allow for detection, not Homologous...targets as well as clonal differences. If you are performing CRISPR library screens, the same principle holds...
Showing: 81 - 100 of 638 results