We narrowed to 270 results for: FAS
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TypeBlog Post... require high sequencing read counts making them fast and viable options for multiplex sequencing in many... for all DSBs, doesn't require high read counts, fast multiplexing Requires delivery of dsODN (potentially... (e.g. dsODN), doesn't require high read counts (fast multiplexing) Requires large cell population, senstive...
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Gamma-Retroviral Vector Guide
TypeGuide...benefit of having well-established protocols for fast and efficient generation of large-scale, high-titer... -
Keeping Up With CRISPR/Cas9
TypeBlog Post... of CRISPR/Cas9 technology are coming along at a fast and furious pace. In December, as we've blogged ...it before, but CRISPRs allow research to move so fast." On the very same day the monkey story hit the ... -
Scientific Sharing in the Time of COVID-19: Databases and Resources
TypeBlog Post...researchers worldwide. “COVID-19 has spread incredibly fast and without any notion of national boundaries. To...policies and ultimately treatments in a timely fashion.” With this in mind, Bourque and Tara Moriarty... -
Sensing Neuronal Dopamine
TypeBlog Post... (e.g., 100 nm) of DA. In addition, they exhibit fast kinetics (10 ms on and 100 ms off), enabling detection...PMCID: PMC426888. Patriarchi, Tommaso, et al. "Ultrafast neuronal imaging of dopamine dynamics with designed... -
Hot Plasmids - October 2022
TypeBlog Post...imaging of voltages. This includes monitoring of fast bursts, as well as slow up/down voltage states in... Sustained deep-tissue voltage recording using a fast indicator evolved for two-photon microscopy. Cell... -
Science Careers: Unruly Interests Feed Many Paths
TypeBlog Post...a simple egg is packed with information. I am fascinated with how developmental programs unfold, absorbing...comparative anatomy showed me that some of the most fascinating biology is found in weird and rare organisms ... -
Natural or synthetic? How Addgene’s dataset reveals trends in biological innovation
TypeBlog Post... the ability to do DNA synthesis, is evolving so fast that it is a really nice opportunity to study it...happens when technological change happens really fast,” Thompson says. To do this, Thompson reached out... -
Learning Technical Skills at a Lab Central Workshop
TypeBlog Post...more about one another. They also provided both breakfast, lunch, and snacks. At the end of the workshop...experiences like workshops at Lab Central. Because of the fast pace of work in the Addgene lab, we don’t often ... -
K. phaffii: Rising to the Occasion
TypeBlog Post...easy to manipulate, it’s affordable, and it grows fast to boot. The yeast strains S. cerevisiae and S. ...to culture and easy to work with Grows extremely fast (doubling time of ~ 2 hours) Amenable to genetic... -
Reagent Repositories Are Speeding up Science During the Pandemic
TypeBlog Post...Scientists need fast access to the tools required to study SARS-CoV-2, but with many academic research... the scientific community. Addgene was awarded a Fast Grant to ensure that the scientific community will... -
Hot Plasmids December 2018 - C. elegans RNAi, TurboID, Detecting Viral Outbreaks, and Protein Binding Specificity Tools
TypeBlog Post...Nucleic Acids Research. 2018 PubMed PMID: 29924347 Faster proximity labeling with TurboID and miniTurbo Article...frequently used for PL were APEX2 and BioID. APEX2 is fast, tagging proximal proteins within minutes, but requires... -
Synthesized by Ginkgo Bioworks, Shared by Addgene: SARS-CoV-2 Plasmids for Many Expression Systems
TypeBlog Post...industrial labs accelerate research and to help find a faster solution to the COVID-19 pandemic. Though we’ve...instrumental in achieving our mission to help science move faster in the middle of a global pandemic. And the reach... -
Protein Tagging with CRISPR/Cas9: A Conversation with Mendenhall and Myers
TypeBlog Post...throughput and faster. It’s also easy to run things in parallel. Addgene: Besides being fast and efficient... -
Form Your Own Peer Mentoring Group: A How-To Guide for Scientists
TypeBlog Post...schedule might work. If not, perhaps a 3 hour breakfast once a month. All participants must commit to ...each other practice meeting people – compete for fastest to set up a coffee date or most cards collected... -
RaPID Detection of RNA-protein Interactions
TypeBlog Post...spatiotemporal resolution for in vivo work. APEX2, a faster peroxidase-based labeling approach, is an alternative...30-fold enrichment compared to E. coli’s BirA*. Faster labeling kinetics also means that BASU could potentially... -
Hot Plasmids - March 2020 - base editors, GEVI, MoClo, and optogenetics
TypeBlog Post...enzyme. Each half of the base editor is fused to a fast-splicing split intein and co-infected into the cell...cyanobacterium Synechocystis sp. PCC6803, and the fast growing strain Synchococcus elongatus UTEX2973, ... -
Summer SciComm: Preprints
TypeBlog Post...pandemic quickly and openly. By sharing results faster and more broadly, scientists can receive recognition...Preprints are all about sharing scientific knowledge faster and more openly — which is also our mission here... -
Summer SciComm Series: Cognitive Load
TypeBlog Post...to mankind. And you probably understand how much faster it would be for an expert in the field to read ...students were fired up and enthusiastic and needed a faster presentation with more information than normal.... -
Molecular Biology Reference
TypeGuide...fitness, bacteria without plasmids can replicate faster and out-populate bacteria with plasmids, thus selecting...