Skip to main content

We narrowed to 323 results for: INO

Showing: 81 - 100 of 323 results
  1. Mycoplasma Contamination: Where Does It Come From and How to Prevent It

    Type
    Blog Post
    ...luminescence. This can then be detected by a luminometer. Non-specific DNA stains can be added in the ...Mycoplasma Removal Agents (MRA) are derivatives of the quinolone family of antibiotics and are broad-spectrum antibiotic...culture media. Also, drugs like BM Cyclin, fluoroquinolone ciprofloxacin, ciprobay, zagam, baytril, tetracycline...
  2. 28 Hot Plasmid Technologies from 2015

    Type
    Blog Post
    ...incorporation of unnatural amino acids (UAAs) into proteins in mammalian cells. Each tRNA/aminoacyl-tRNA synthase ...conjugation reactions, fluorescent amino acids, posttranslationally modified amino acids, photoaffinity probes...  These plasmids are unpublished Unnatural amino acid incorporation in mammalian cells Peter Schultz...
  3. Typing CRISPR Systems

    Type
    Blog Post
    ... are among the smallest Cas enzymes (400 to 700 amino acids), use a Cas14 enzyme, and uniquely target ...Harrington, L. B., Burstein, D., Chen, J. S., Paez-Espino, D., Ma, E., Witte, I. P., Cofsky, J. C., Kyrpides...Fedorova, I., Karneyeva, K. A., Artamonova, D. N., & Severinov, K. V. (2021). Type III CRISPR-CAS systems: deciphering...
  4. KLF Research Plasmids

    Type
    Collection
    ... metabolism, fibrosis, atherosclerosis, and carcinogenesis. The defining feature of the KLF family is ...
  5. CRISPR 101: Cytosine and Adenine Base Editors

    Type
    Blog Post
    ... is the nucleobase component of the nucleoside inosine. Created with BioRender.com. How does base ...adenine base editor that would convert adenosine to inosine, resulting in an A to G change (Gaudelli et al....non-G conversion at target loci. The removal of inosine from DNA is likely infrequent, thus preventing ...
  6. Chemogenetics Plasmids

    Type
    Collection
    ...Another recently developed class of tools are Luminopsins (LMOs), or luminescent opsins: fusion proteins...
  7. CRISPR Plasmids and Resources

    Type
    Collection
    ...Plasmids and Resources CRISPR technologies are continously developing and expanding. Addgene works with ...
  8. CRISPR Plasmids - Base Edit

    Type
    Collection
    ...editors have been engineered to convert adenosine to inosine, which is treated like guanosine by the cell, creating...
  9. SARS-CoV-2 Pseudotyped Virus

    Type
    Collection
    ... optimization, deletion of the C-terminal 18-21 amino acids and a D614G change which is present in many...
  10. Celebrating the 2024 Nobel Prize in Chemistry

    Type
    Blog Post
    ...” diagrams, which show the interactions between amino acids in an approximate, two-dimensional map. The...topology, then iteratively generated 3D models and amino acid sequences that got closer and closer to achieving...
  11. Streaking for Single Colonies: The Streak Plate Challenge

    Type
    Blog Post
    ...successful streak plate, first the bacteria are inoculated over a quadrant of an agar plate. Then, a stick...Plate Challenge, Westenberg’s course includes Winogradsky columns and characterizing the microbes from ...
  12. Six Spooky Science Stories and Halloween at Addgene

    Type
    Blog Post
    ...1983). Grasshopper parasite The parasitic worm Spinochordodes tellinii‘s larvae makes its home inside grasshoppers...Drosophila melanogaster." Molecular and cellular endocrinology 215.1-2 (2004): 1-10. PubMed PMID: 15026169....
  13. Promega Plasmid Collection

    Type
    Collection
    ...small complementary peptide, Small BiT (SmBiT; 11 amino acids). This system is ideal for protein:protein...
  14. Plasmids 101: Origin of Replication

    Type
    Blog Post
    ...conditions aren't working against you!  The culture inoculum Freshly streaked bacteria have higher copy numbers...versatile platform for single- and multiple-unnatural amino acid mutagenesis in Escherichia coli. Biochemistry...
  15. In Vivo Biotinylation of Bacterial Fusion Proteins

    Type
    Blog Post
    ...generate covalent bonds with positively-charged amino acids. Unfortunately, the location of biotin incorporation...enzyme biotin ligase, which binds to a small (< 20 amino acid) AviTag sequence in a protein and adds biotin...
Showing: 81 - 100 of 323 results