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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 326 results for: INO

Showing: 81 - 100 of 326 results

  1. Celebrating the 2024 Nobel Prize in Chemistry

    Type
    Blog Post
    ...” diagrams, which show the interactions between amino acids in an approximate, two-dimensional map. The...topology, then iteratively generated 3D models and amino acid sequences that got closer and closer to achieving...

  2. Streaking for Single Colonies: The Streak Plate Challenge

    Type
    Blog Post
    ...successful streak plate, first the bacteria are inoculated over a quadrant of an agar plate. Then, a stick...Plate Challenge, Westenberg’s course includes Winogradsky columns and characterizing the microbes from ...

  3. Six Spooky Science Stories and Halloween at Addgene

    Type
    Blog Post
    ...1983). Grasshopper parasite The parasitic worm Spinochordodes tellinii‘s larvae makes its home inside grasshoppers...Drosophila melanogaster." Molecular and cellular endocrinology 215.1-2 (2004): 1-10. PubMed PMID: 15026169....

  5. In Vivo Biotinylation of Bacterial Fusion Proteins

    Type
    Blog Post
    ...generate covalent bonds with positively-charged amino acids. Unfortunately, the location of biotin incorporation...enzyme biotin ligase, which binds to a small (< 20 amino acid) AviTag sequence in a protein and adds biotin...

  7. Plasmids 101: Origin of Replication

    Type
    Blog Post
    ...conditions aren't working against you!  The culture inoculum Freshly streaked bacteria have higher copy numbers...versatile platform for single- and multiple-unnatural amino acid mutagenesis in Escherichia coli. Biochemistry...

  8. Another Pathway into Cells: iTOP

    Type
    Blog Post
    ...studies showed that iTOP functions through macropinocytosis. D’Astolfo et al. tested iTOP in a series... cultured mammalian cells by osmotic lysis of pinocytic vesicles. Okada CY, Rechsteiner M. Cell. 1982 ...

  11. Hot Plasmids - February 2022

    Type
    Blog Post
    ...only requires a small epitope tag (FLAG is eight amino acids, HA is nine), multiple repeats of the tag ...editing and gene activation, is composed of only 529 amino acids, less than half the size of either Cas9 or...

  12. Fluorescent Proteins 101: Luciferases

    Type
    Blog Post
    ...best known: it’s derived from the small (19 kDa) luminous shrimp (Oplophorus gracilirostris) luciferase ...Luciola cruciata) Natural 562 64 Luciferin ATP No Luminous shrimp(Oplophorus gracilirostris) Natural 455 ...

  13. Antibodies 101: Monoclonal Antibodies

    Type
    Blog Post
    ...a small peptide that represents a short, unique amino acid sequence found in the protein, or, are injected...a single epitope, so it’s less likely that this amino acid sequence will be shared across other proteins...

  14. Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs

    Type
    Blog Post
    ...gRNAs used the RNA nuclease Csy4 from Pseudomonas aeruginosa to cleave 4 gRNAs separated by 28 base Csy4 recognition...processing to make gRNAs In a recently published paper, Yinong Yang’s group did just that as part of their CRISPR...

  17. Antibodies 101: Conjugation

    Type
    Blog Post
    ... antibodies, such as inclusion of non-canonical amino acids or peptide tags. 5) Strong protein-protein...Reisfeld RA (1988) Determination of the Number of e-Amino Groups Available for Conjugation of Effector Molecules...

  20. Quickest Way to Deposit Plasmids: The Deposit Spreadsheet

    Type
    Blog Post
    ...” column. Relevant Mutations Indicate the amino acid change as well as any functional consequences...hygromycin, kanamycin, nourseothricin (clonNat), spectinomycin, streptomycin, tetracycline, combinations of...
Showing: 81 - 100 of 326 results