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Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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In Category

We narrowed to 319 results for: INO

Showing: 81 - 100 of 319 results

  2. Celebrating the 2024 Nobel Prize in Chemistry

    Type
    Blog Post
    ...” diagrams, which show the interactions between amino acids in an approximate, two-dimensional map. The...topology, then iteratively generated 3D models and amino acid sequences that got closer and closer to achieving...

  3. TALEN Expression Vectors

    Type
    Collection
    ...TAL effector repeat arrays with identical DNA and amino acid sequences (i.e. only the details of the assembly...epitope tag and nuclear localization signal at their amino-terminus. The expression vectors listed below can...

  4. CRISPR Plasmids - RNA Editing

    Type
    Collection
    ...RNA editor that converts adenosine to inosine in RNA. Since inosine is functionally equivalent to guanosine...

  5. Brain Initiative Collection

    Type
    Collection
    ...for Cre-dependent expression of Venus-tagged parapinopsin driven by the CAG promoter. Used for photoswitchable...for Cre-dependent expression of Venus-tagged parapinopsin driven by the Ef1a promoter. Used for photoswitchable...

  6. Streaking for Single Colonies: The Streak Plate Challenge

    Type
    Blog Post
    ...successful streak plate, first the bacteria are inoculated over a quadrant of an agar plate. Then, a stick...Plate Challenge, Westenberg’s course includes Winogradsky columns and characterizing the microbes from ...

  7. Six Spooky Science Stories and Halloween at Addgene

    Type
    Blog Post
    ...1983). Grasshopper parasite The parasitic worm Spinochordodes tellinii‘s larvae makes its home inside grasshoppers...Drosophila melanogaster." Molecular and cellular endocrinology 215.1-2 (2004): 1-10. PubMed PMID: 15026169....

  9. mTOR Pathway

    Type
    Collection
    ...better-characterized mTORC1 responds to various signals, including amino acids, stress, oxygen, energy levels, and growth...PIK3R1 PIK3R2 PIK3R3 PIK3R4 PIK3R5 PIK3R6 Phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic and ...

  11. In Vivo Biotinylation of Bacterial Fusion Proteins

    Type
    Blog Post
    ...generate covalent bonds with positively-charged amino acids. Unfortunately, the location of biotin incorporation...enzyme biotin ligase, which binds to a small (< 20 amino acid) AviTag sequence in a protein and adds biotin...

  12. Plasmids 101: Origin of Replication

    Type
    Blog Post
    ...conditions aren't working against you!  The culture inoculum Freshly streaked bacteria have higher copy numbers...versatile platform for single- and multiple-unnatural amino acid mutagenesis in Escherichia coli. Biochemistry...

  13. Another Pathway into Cells: iTOP

    Type
    Blog Post
    ...studies showed that iTOP functions through macropinocytosis. D’Astolfo et al. tested iTOP in a series... cultured mammalian cells by osmotic lysis of pinocytic vesicles. Okada CY, Rechsteiner M. Cell. 1982 ...

  14. Microbiology Resources

    Type
    Collection
    ...Campylobacter sp. Chlamydia sp. Clostridium sp. Deinococcus radiodurans Enterococcus sp. Geobacillus sp. ...assembly of up to three transcription units in the oleaginous yeast Y. lipolytica - Rossignol Lab Yeast Gateway...

  15. TALEN Guide

    Type
    Collection
    ...composed of 33-35 amino acid repeats. These repeats only differ from each other by two amino acids, their ...

  18. Antibodies 101: Monoclonal Antibodies

    Type
    Blog Post
    ...a small peptide that represents a short, unique amino acid sequence found in the protein, or, are injected...a single epitope, so it’s less likely that this amino acid sequence will be shared across other proteins...

  19. Hot Plasmids - February 2022

    Type
    Blog Post
    ...only requires a small epitope tag (FLAG is eight amino acids, HA is nine), multiple repeats of the tag ...editing and gene activation, is composed of only 529 amino acids, less than half the size of either Cas9 or...

  20. Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs

    Type
    Blog Post
    ...gRNAs used the RNA nuclease Csy4 from Pseudomonas aeruginosa to cleave 4 gRNAs separated by 28 base Csy4 recognition...processing to make gRNAs In a recently published paper, Yinong Yang’s group did just that as part of their CRISPR...
Showing: 81 - 100 of 319 results