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We narrowed to 492 results for: LARGE;

Showing: 81 - 100 of 492 results

  1. New CRISPR Tools: Cas7-11 and PASTE

    Type
    Blog Post
    ...III-E, and then used this information to identify a large variety of related bacteria with similar Cas systems...fused integrase would then integrate into with a large DNA insert – or Programmable Addition via Site-specific...programmed for new genes. In comparison with other large insert techniques like HITI or HDR, which require... and adenovirus templates. The system, which is larger than Cas9, can also be used with mRNA and a synthetic...

  2. Lentiviral Vector Uses and Overview

    Type
    Blog Post
    ... in the lab. Advantages of lentivirus include a large genetic capacity and the ability to transduce both...the clinic Since lentiviral vectors can deliver a large amount of DNA (~8 kb) with a relatively low immune...lentiviral vectors for applications that require the large genetic capacity of a lentiviral vector but for ...Dorazio R, Mulder GJ, Polack A, Bank A, Soulier J, Larghero J, Kabbara N, Dalle B, Gourmel B, Socie G, Chrétien...

  3. Plasmids 101: Protein tags

    Type
    Blog Post
    ...detection; however, those are just one (admittedly large) class of common fusion protein tags. Biochemists...; has inherent enterokinase cleavage site GST Large Protein 26 Purification and Stability Good for purification..., flanked by hexahistidine motifs (6xHis) MBP Large Protein  40 Solubility and Purification Can improve...

  4. Plasmids 101: Dimers and Multimers

    Type
    Blog Post
    ...observed to occur more frequently in plasmids with large inserts (>7 kb)(Berg et al., 1989), direct repeats...reads (<300 bp) which cannot distinguish between large repeat sequences within a plasmid, it cannot be ...sequencing. These longer reads are able to detect large repeat sequences within a plasmid and, therefore...DE. pBR322-derived multicopy plasmids harboring large inserts are often dimers in Escherichia coli K-12...just as well as monomers. However, due to their larger size, multimers may exhibit reduced transformation... & Sherratt, 1984; Summers et al., 1993). These larger plasmids are also maintained at lower copy numbers...monomeric version. However, undigested multimers are larger than monomers and will therefore run higher on ...

  5. Prime Editing: Adding Precision and Flexibility to CRISPR Editing

    Type
    Blog Post
    ... that cellular mismatch repair contributed to a large number of unintended prime editing outcomes, including...combine prime editing with a recombinase to install large sequences (up to 10 kb) into the genome (Pandey ...deletion, replacement, integration and inversion of large DNA sequences with twin prime editing. Nature Biotechnology... (2024). Efficient site-specific integration of large genes in mammalian cells via continuously evolved...J. S. (2023). Drag-and-drop genome insertion of large sequences without double-strand DNA cleavage using...

  6. Troubleshooting and Optimizing a Western Blot

    Type
    Blog Post
    ...overheating remains an issue, which can happen with large proteins, try lowering the voltage and using a longer...you know you loaded a good amount of sample of a large protein, you may be under-transferring.  Membrane...good indication that the p membrane pores are too large.  Transfer method   Wet transfers are more efficient...efficient, so if you’re struggling to transfer a large protein, consider using a wet transfer with a long... lot of antibodies. If you’re planning to use a large amount of a specific antibody, plan ahead and request...harsher stripping buffer Too little signal Large proteins: Incomplete transfer Check transfer with...proteins going completely through the membrane or larger proteins not transferring completely onto the membrane...

  7. Antibody Plasmid Collection

    Type
    Collection
    ...Expressing Antibodies Antibody Plasmids Antibodies are large proteins made by the immune system that bind to ...

  9. New and Upcoming Viral Vectors - Spring 2019

    Type
    Blog Post
    ...fluorescence. It is useful for activity imaging of large populations of densely labeled neurons because background...fluorescence.  It is useful for activity imaging of large populations of densely-labeled neurons because background...accumulates in the endoplasmic reticulum, but exhibits larger fluorescent responses to GABA. Find the iGABASnFR...

  10. Plasmids 101: What is a plasmid?

    Type
    Blog Post
    ...plasmids as they grow, scientists can easily make large amounts of plasmid to manipulate and use in later...that plasmids can be used to do include: Produce large amounts of a protein so that scientists can purify...

  12. Luciferase Plasmid Collection

    Type
    Collection
    ...but are less-than-ideal protein tags due to their large size (61 kDa and 36 kDa, respectively). And they...new window) : The NanoLuc® enzyme is split into Large (LgBiT; 18 kDa) and Small (SmBiT; 11 amino acids...

  13. Replacing Paper: Tips for Choosing an Electronic lab Notebook

    Type
    Blog Post
    ...solution that fits the most labs, our team conducted a large study which focused on the adoption of ELNs in labs...talking about ELN costs and limited budgets, “a large percentage of survey respondents indicated that ...support that. In addition, if you are working for a large institution or company, site licenses might be available...

  14. Adeno-associated Viruses (AAVs) for Genome Editing

    Type
    Blog Post
    ...to efficiently deliver large repair templates for applications that require large gene fusions. The current...Matthew H. Porteus. "CRISPR-mediated integration of large gene cassettes using AAV donor vectors." Cell reports...

  15. Twenty Years of Sharing: Addgene's Viral Vector Service

    Type
    Blog Post
    ...and to verify the quality of the prep. This was a large factor in Addgene’s decision to launch our viral...category, available from Addgene as of 2023. A large percentage of viral vector preps contain biosensors...were distributed in 2023 alone. You’ll also find a large number of requests for vectors in the “controls”...

  16. Harnessing TnpB for Genome Editing: A Compact and Versatile Tool for Your Research

    Type
    Blog Post
    ...toolbox. However, both of these proteins are quite large — comprising approximately 1000–1400 amino acids... cell types, it has a limited capacity to carry large DNA cargos. As a result, the search for smaller,...capacity, which makes it difficult to accommodate the large coding sequence of commonly used nucleases like ...

  17. CRISPR Activation: A Practical Guide

    Type
    Blog Post
    ...guides directed towards that gene as there can be a large difference in guide potency. Thus, in cases where...pathways and genetic interactions. Additionally, large scale CRISPR-based screens can be used to gain insight...target effects are generally not regarded as being a large problem for Cas9 activators. This is believed to...makes it much cheaper to use Cas9 activators. This larger scale of activation allows for exploratory-based...

  18. Site Directed Mutagenesis by PCR

    Type
    Blog Post
    ...more efficiently amplified than larger constructs, but plasmids as large as ~6 kb can be amplified fairly... This is particularly relevant in cases where a large plasmid with multiple functional regions is used... if you want to introduce a point mutation in a large construct. Particularly, if a pair of unique restriction...visible in a digest of the unmodified plasmid into a larger band (not shown). As a footnote; a similar approach...site, can identify these duplications (slightly larger bands sizes relative to the original template)....
Showing: 81 - 100 of 492 results