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We narrowed to 1,038 results for: LAS

Showing: 81 - 100 of 1038 results

  1. Plasmids 101: Golden Gate Cloning

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    Blog Post
    ...Addgene’s plasmids are used with a wide variety of restriction enzyme-based cloning methods. Each method...Entry DNA overhangs may be present in the original plasmid (Option 1) or added using PCR-based amplification...fragments to create TAL effector nucleases. These plasmids were designed to utilize the BsaI and BsmBI Type... a gRNA target sequence into a Cas9-containing plasmid such as pX330. This cloning strategy not only makes...makes it easy to create a single gRNA-expressing plasmid, but it can also be adapted to express multiple...create CRISPR/Cas9 constructs, assemble standard plasmids parts in different combinations, or other new ...amplification of cloned DNA as tandem multimers using class-IIS restriction enzymes. Lee JH, Skowron PM, Rutkowska...

  2. Plasmids 101: Blue-white Screening

    Type
    Blog Post
    ...This post is part of our ongoing Plasmids 101 series. Plasmids 101 will provide you with an overview ...of the Plasmids 101 posts here. Now that we have covered antibiotic selection here at Plasmids 101, we...α-complementation plasmid. This way you can quickly and easily identify colonies that not only have your plasmid (antibiotic...rescued by genes supplied on the transformed plasmid. Such plasmids may contain genes that allow for the use... able to select for colonies that contain your plasmid is a great start when cloning, but how about being...being able to choose those that contain plasmid with an insert? Blue-white selection is a widely used ...far, so good, but how does this help us screen plasmids? Blue-white screening in the lab Scientists discovered...

  3. 22 Hot Plasmid Technologies from 2014

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    Blog Post
    ... Hot Plasmid list. Other popular plasmids & blog posts @Addgene: The 10 Most Distributed Plasmid Technologies...inducible inactivation. Find the Robinson plasmids or the Royle plasmids and get to work! Robinson et al...designed the pOSIP (one-step integration plasmid) series of plasmids, vectors that convey both the sequence...mGRASP plasmids. Additionally, the lab recently described the use of another set of mGRASP plasmids in their... favorite plasmid technologies? Email us at [email protected]  and let us know what plasmids you would ...sgRNA libraries expand upon the CRISPR family of plasmids by offering a lentivirus-based mechanism for sgRNA...new CRISPR screening tools, see our CRISPR/Cas Plasmids: Pooled Libraries webpage or read our blog post...

  4. Hot Plasmids: FRET-Based Biosensors

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    Blog Post
    ...) Interested in More Hot Plasmids from Addgene? Check out our Hot Plasmid page, where we highlight some...popular & newly available plasmids at Addgene. If you're interested in plasmids for in vivo imaging, check...the greatest challenges of neuroscience.”  Hot plasmids: Twitch sensors Griesbeck’s recent contribution...

  5. Your Lentiviral Plasmid FAQs Answered

    Type
    Blog Post
    ...four different plasmids into the producer cells (two packaging plasmids, an envelope plasmid, and the lentiviral...generation systems consist of an envelope plasmid and a packaging plasmid encoding HIV proteins gag, pol, rev...generation systems require an envelope plasmid, and two packaging plasmids, one encoding gag and pol and a second...transfer plasmids replication competent? What is SIN? A6: Most (if not all) Addgene transfer plasmids are ... 30% of the transfer plasmid. Many transfer vectors are SIN. Packaging plasmid(s) contain the minimal...Addgene's most popular lentiviral plasmids Overview of Lentiviral Packaging Plasmids Lentiviral Protocols and ...Addgene has an extensive collection of lentiviral plasmids created for a variety of applications including...

  6. Plasmids 101: The Promoter Region – Let's Go!

    Type
    Blog Post
    ...Once you've gotten to know what a plasmid is in general, it's time to take a look at some of its parts...origin of replication are necessary to replicate plasmids and make sure the cells maintains it. Here, we'll...the promoter, which is necessary for getting the plasmid express our gene of interest. This element is responsible.... This means that the promoter present in your plasmid backbone must to be compatible with the type of...transcript, you will also need to make sure your plasmid has a promoter suited to working in your host organism...development-specific promoters available to scientists. Plasmids are oftentimes put to therapeutic uses, and in...Additional Resources on the Addgene Blog Browse All Plasmids 101 Posts Learn about Mammalian Vectors Read about...

  7. Plasmids 101: Antibiotic Resistance Genes

    Type
    Blog Post
    ...those bacteria with a pressure to keep your plasmid. Viva la (bacterial) resistance!  What are antibiotics... presence of a plasmid is disadvantageous from the bacterium's perspective – a plasmid-containing cell...to maintain the plasmid. Adding an antibiotic resistance gene to the plasmid solves both problems at once...Biology Reference Pages View our Plasmid Protocols Find Your Perfect Plasmid Backbone ...think about how much easier it makes our lives. Plasmid transformation into E. coli is a fairly inefficient... which cells successfully received the correct plasmid, scientists would spend hours to days trying find...cell must replicate the plasmid in addition to its own chromosomal DNA, costing additional resources to ...

  8. Plasmids 101: Modular Cloning Applications and Kits

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    Blog Post
    ...between CRISPR components expressed from different plasmid backbones. They noted that backbone differences... as the order, or distance in the placement of plasmid elements can impact expression levels and consequently...system was used to assess how different lentiviral plasmid backbone elements impact viral titer and to compare...plants, and animals (Chiasson et al., 2019). Many plasmid elements such as promoters or fluorophores are ...Collection More resources on the Addgene blog Plasmids 101: Golden Gate Cloning High Complexity Golden...

  9. Open Resources and Plasmid Tools For Studying C. elegans

    Type
    Blog Post
    ...RNA-sequencing to identify neuronal class and deep sequencing of neurons from each class to fully characterize the... CRISPR plasmid can be created in a single tube. You can also find additional SapTrap plasmids used for...elegans Browse our Plasmids 101 blog posts Resources on Addgene.org Visit our worm plasmids and resources... databases for scientists including Wormbook, Wormatlas and Wormbase. And scientists have been continuously...discussed at the meeting, in addition to useful plasmids and toolkits available at Addgene.  C. elegans...is to establish a comprehensive gene expression atlas of every C.elegans  neuron. Scientists are currently... development (Kyoda et al., 2012).  C. elegans plasmid tools at Addgene In addition to the wealth of open...

  10. Hot Plasmids and Viral Preps - January 2021

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    Blog Post
    ...subset of the new plasmids and viral preps in the repository through our hot plasmids articles. These articles...recent plasmid deposits and we hope they'll make it easier for you to find and use the plasmids you need...highlights from recent CRISPR plasmids. To find all of the CRISPR plasmids available from Addgene, head...AAV-deliverable ABE.  Find the plasmid here Jesse Zalatan’s lab deposited plasmids for a chemically inducible...need. If you'd ever like to write about a recent plasmid deposit please sign up here.  Here's what you'll...mGold, a new yellow fluorescent protein New CRISPR plasmids New items from the viral service A fast blue-light...protein.    Cells expressing an RFP reporter plasmid for recombinase activity were exposed to blue light...

  11. Plasmids 101: Gene, enhancer, and promoter trapping

    Type
    Blog Post
    ...in the Lab Plasmids 101: Screens vs. Selections Plasmids 101: Knockout /Knock-In Plasmids ...the Lab Bench Plasmids 101 e-book Additional resources on the Addgene blog Plasmids 101: Using Transposons...

  12. Hot Plasmids and Viral Preps - September 2021

    Type
    Blog Post
    ...subset of the new plasmids and viral preps in the repository through our hot plasmids articles. These articles...recent plasmid deposits and we hope they'll make it easier for you to find and use the plasmids you need... CRISPR plasmids are always being added to the repository. To find all of the CRISPR plasmids available... Xiao Wang Lab has recently deposited a set of plasmids that helps identify base edited cell populations...populations without end-point sequencing assays. These plasmids use “Cas9-mediated adenosine transient reporter...available from Addgene, head over to our CRISPR Plasmids and Resources page. Here are some highlights from the...Nasse We regularly add new viral aliquots from our plasmid collection to provide ready-to-use viral preps....

  13. Hot Plasmids and Viral Preps - July 2021

    Type
    Blog Post
    ...subset of the new plasmids and viral preps in the repository through our hot plasmids articles. These articles...recent plasmid deposits and we hope they'll make it easier for you to find and use the plasmids you need... CRISPR plasmids are always being added to the repository. To find all of the CRISPR plasmids available...EasyTag workflow combines thoughtfully designed plasmid components with Fluorescence Activated Cell Sorting...further demonstrated that when tested in tobacco protoplasts, the enzymatic biosensor retains its catalytic...available from Addgene, head over to our CRISPR Plasmids and Resources page. Here are some highlights from the...Nasse We regularly add new viral aliquots from our plasmid collection to provide ready-to-use viral preps....

  14. Hot Plasmids and Viral Preps - May 2021

    Type
    Blog Post
    ...subset of the new plasmids and viral preps in the repository through our hot plasmids articles. These articles...recent plasmid deposits and we hope they'll make it easier for you to find and use the plasmids you need...highlights from recent CRISPR plasmids. To find all of the CRISPR plasmids available from Addgene, head...Hutchinson Cancer Research Center developed a pooled plasmid library encompassing all possible amino acid mutations...anti-SARS-CoV-2 antibodies. In the library, each plasmid encodes the mutated spike protein and a barcode...head over to our CRISPR Plasmids and Resources page.  A new system uses light to deactivate photocleavable... accumulation of indels to measure time. These plasmids with different promoters driving expression of...

  15. Hot Plasmids and Viral Preps - March 2021

    Type
    Blog Post
    ...subset of the new plasmids and viral preps in the repository through our hot plasmids articles. These articles...recent plasmid deposits and we hope they'll make it easier for you to find and use the plasmids you need...highlights from recent CRISPR plasmids. To find all of the CRISPR plasmids available from Addgene, head...head over to our CRISPR Plasmids and Resources page.  Plasmids for delivering CRISPRi components by a single...inducible degron system for C. elegans New CRISPR plasmids New items from the viral service Epigenetic ... Haynes at Emory recently contributed a set of plasmids to the repository that will help epigenetic researchers...researchers custom design their own engineering plasmid tools in about a week. Taking advantage of Golden...

  16. Plasmids 101: Gram Negative and Positive Bacteria

    Type
    Blog Post
    ...Addgene blog Plasmids 101: What is a plasmid? Plasmids 101: Blue-white screeningPlasmids 101: Common lab...bacteria are permeable to different classes of antibiotics. The classification system is also useful in a laboratory... the plasmid should change based on bacteria type. The gram positive and negative classification system...ll be talking about the ins and outs of this classification system, including its history, biology, and...repeatable phenomenon, Gram began using it as a classification system. Eventually, any bacteria that retained...laboratory setting. When designing a plasmid to be inserted into a specific bacterium, it can be important...

  17. New FLIS Plasmid (April Fools 2022)

    Type
    Blog Post
    ...inconspicuous coloring.  FLIS plasmid Recently, the Phuels lab deposited a plasmid that will forever change...lab References Abril, U.N.O., et. al. (2022) A plasmid-based system for locating and containing escaped...

  18. Plasmids 101: Expressing shRNAs for RNA Interference

    Type
    Blog Post
    ...comparison of an shRNA plasmid sequence and an miRNA plasmid sequence. (A) Plasmid sequence from pLVUHshp53....   Expressing shRNAs using plasmids The design of shRNA plasmids requires three important steps: ...between the red boxes. (B) Plasmid sequence from pcDNA3-miR21 (Addgene plasmid #21114). The miRNA sequence...shRNA plasmids are being designed using RNA polymerase II promoters. The design of these plasmids is meant...with Cas13a (C2c2) Plasmids 101: The Promoter Region – Let's Go! Browse All Plasmids 101 Posts Additional... occur via transfection or viral delivery of a plasmid. RNAi is an umbrella term for a number of different...Following delivery into cells using viral vectors or plasmids, shRNAs hijack the endogenous miRNA machinery ...

  19. Plasmids 101: Choosing an Antibiotic Resistance Gene

    Type
    Blog Post
    ...resistance DNA plasmids are traditionally grown in bacteria as a way to produce more of the plasmid, isolate...isolate individual plasmids, or express a gene contained in a plasmid. During the propagation and/or transformation...receive a copy of the plasmid or will lose it during division. These non-plasmid-containing bacteria can...Over-Agar Antibiotic Plating Plasmids 101 eBook Resources on the Addgene blog Plasmids 101: Antibiotic Resistance...Resistance Plasmids 101: Blue-White Screening Choosing Your Perfect Plasmid Backbone  ...Plasmids need antibiotic resistance genes so that you can specifically isolate bacteria containing your...your experiment — to grow, express, or isolate plasmids. The presence of an antibiotic resistance gene...

  20. "Build Your Plasmid": The Game - Play to Teach

    Type
    Blog Post
    ... educational tools Playing BUILD YOUR PLASMID BUILD YOUR PLASMID is a game where you need to apply basic... videos to teach basic lab techniques Plasmid Land: The plasmid cloning game ...Barcelona wanted to develop strategies to make our classes more practical and hands-on. Students in these ...daughters playing with some cards and I had a quick flashback to time spent playing a card game called “Magic... gene and Selection Markers) and one “Transfer Plasmid” game board. Once they agreed on each element to...minutes to develop a strategy and build a transfer plasmid that could be used to genetically manipulate human...for the disease to be openly discussed with the class. In this way, the students also had to practice ...
Showing: 81 - 100 of 1038 results