We narrowed to 178 results for: Lta;

1. Pushing the Limits of DNA Assembly

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   Blog Post

   ...desired mutations. This same process can be used simultaneously for the generation of one or more mutations...

2. Quick Guide to Working with Drosophila Part 2: Controlling Gene Expression in Flies with Gal4/UAS

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   ...compartment, you could express them individually or simultaneously. Do note for control purposes that there are...

3. The Power Behind NGS Plasmid Validation: seqWell

   Type

   Blog Post

   ...here is that, as more samples are sequenced simultaneously, it gets harder and harder to obtain balanced...

4. Plasmids 101: Mammalian Vectors

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   ...mammalian cells is fairly straightforward and the resultant cells can either express the plasmid DNA transiently...

5. Biosensor AAV Preps

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   ...Sensors GRAB_NE nLight Oxytocin (OT) Sensors GRAB_OT Voltage Sensors Archon Voltron JEDI-2P ATP Sensors iATPSnFR2...GRAB_OTmut (control) none Constitutive 1, 9 Li Voltage Reporter: Archon 108422 pAAV-CAG-FLEX-Archon1-KGC-EGFP-ER2...KGC-GFP-ER2] Syn Archon1 EGFP Cre dependent 8 Boyden Voltage Reporter: Voltron 119036 pAAV-hsyn-flex-Voltron-ST...ST-WPRE Syn Voltron2-ST none Cre dependent 1 GENIE Voltage Reporter: JEDI-2P 179459 pAAV-EF1a-DIO-JEDI-2P-...

6. Expanding the Targeting Scope and Editing Efficiency of Adenine Base Editors

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   ...fetal hemoglobin gene, ABE8e made both changes simultaneously with 54% efficiency, about 7 times higher than...

7. Multiplex Genome Editing with CRISPR-Cpf1

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   Blog Post

   ... vivo, transfection, viral transduction) for simultaneous multi-gene editing with a single crRNA array...

8. Viral Vectors 101: Biosensors

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   ...Cellular phenomena such as exocytosis, autophagy, voltage, and tension all have specific sensors designed...

9. Viral Vectors 101: Viral Applications

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   Blog Post

   ...Libraries allow you to test thousands of elements simultaneously in your system instead of just one at a time...

10. Opto-Nanobodies: Using Light to Manipulate Cell Signaling and Protein Purification

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    Blog Post

    ...domain and a nanobody.  AsLOV2 is a light-oxygen-voltage sensing domain from the oat plant (Avena sativa...

11. A Deep Dive into BioBuilder

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    Blog Post

    ...Mary Tamer is a Boston-based writer, editor, and consultant whose work is focused on education and education...

12. CRISPR 101: Non-Homologous End Joining

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    Blog Post

    ...regions of hundreds of base pairs or more can simultaneously introduce a pair of chromosome breaks, and ...

13. Pooled CRISPR Libraries Offer Genome-Wide Control for Large-Scale Functional Screens

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    ...Activation. Gilbert LA, Horlbeck MA, Adamson B, Villalta JE, Chen Y, Whitehead EH, Guimaraes C, Panning...

14. Channelrhodopsins with improved light sensitivity for minimally-invasive optogenetics

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    Blog Post

    ... and spectral properties, must be optimized simultaneously in order to generate a channelrhodopsin that...

15. Antibodies 101: Affinity Reagents

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    Blog Post

    ...different antigen; binding of each can be either simultaneous or independent of the other (Shahied, 2004)....

16. Tagging Optogenetics and Chemogenetics Receptors: Fluorescent Proteins and Other Options

    Type

    Blog Post

    ...example green and red fluorescent proteins) allow simultaneous identification of more than one protein of interest...

17. CRISPRainbow and Genome Visualization

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    ...By labeling two genes of a given chromosome simultaneously, they could also monitor the spatial relationship...

18. Antibodies 101: Conjugation

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    ...M, Hafemeister C, Stephenson W, et al (2017) Simultaneous epitope and transcriptome measurement in single...

19. Pairing CombiGEM and CRISPR for Combinatorial Genetic Screening

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    ...particular phenotype, and quickly profile the resultant hits (Cheng et al., 2014; Wong et al., 2015; Wong...

20. Career Coaching for Scientists: Why and Where Do I Find One?

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    Blog Post

    ...A professional coach will provide an initial consultation meeting or call for free.  Take advantage of...