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We narrowed to 764 results for: Mos

Showing: 81 - 100 of 764 results

  1. AAV Titration by qPCR Using SYBR Green Technology

    Type
    Protocol
    ...green are the ones loaded onto the qPCR plate for most samples. If sample is expected to have a titer <...have some small amount of background signal that is most evident during initial PCR cycles. This background...

  2. Pouring LB Agar Plates

    Type
    Protocol
    ...Although our recommended temperature should kill most potential contaminants, certain types of spores ... agar will remain liquid at this temperature but most antibiotics will not break down at this temperature...

  3. Validated gRNA Sequences

    Type
    Collection
    ...GAGTCCGAGCAGAAGAAGAA 69236 cut S. pyogenes 26480473 Wolfe ttTi5605 Mos1 insertion site C. elegans ATATCAGTCTGTTTCGTAA 47550...

  4. Kit Free RNA Extraction

    Type
    Protocol
    ...is. While simple homogenization is effective for most mammalian tissues; more hardy tissues such as bone...

  5. Cre-lox system

    Type
    Collection
    ...pMAZe Reporter construct for lineage tracing and mosaic analysis in zebrafish Zebrafish Lewis Additional...

  6. Immunology Research Plasmids and Resources

    Type
    Collection
    ...SMAP, SMAP2, p120 BTC betacellulin - C19orf10 chromosome 19 open reading frame 10 EUROIMAGE1875335, IL25... CD27L, CD27LG, TNFSF7 CECR1 cat eye syndrome chromosome region, candidate 1 ADGF, IDGFL CER1 cerberus...

  7. DNA Quantification

    Type
    Protocol
    ...different wavelengths of light to varying degrees and most have a specific wavelength that they maximally absorb...

  9. Water Bath Protocol

    Type
    Protocol
    ...and room temperature. This general protocol suits almost any water bath. Video Watch the video for tips ...

  15. Western Blot

    Type
    Protocol
    ...protein of interest. RIPA buffer is suitable for most proteins but more stringent buffers and a sonication...

  17. Quick Guide to Working with Drosophila Part 1: Getting Started with Flies

    Type
    Blog Post
    ...genome. They have four chromosomes: the X (and Y), second, third, and fourth chromosomes. Also, the fourth ...third chromosomes are the ones you’ll work with. If YFG is somehow on the Y or fourth chromosomes, you ...geneticists use balancer chromosomes to prevent genetic recombination. Balancer chromosomes essentially are messed...the start of the day and throughout daytime. They mostly stop emerging at night. An incubator with a light...fourth and Y chromosomes are miniscule, so researchers generally do not manipulate them. Essentially, the X... messed up chromosomes with multiple inversions. They are unable to participate in genetic recombination... viable if they are homozygous for a balancer chromosome. Thus, you can stably maintain a heterozygote...

  18. Five Popular Model Organisms, Part 2

    Type
    Blog Post
    ...tropicalis, the two most commonly used species of frog. (Liu et al., 2016). Some of the most popular tools ...emerging model organisms!) Escherichia coli One of the most recognizable and utilized model organisms is Escherichia... organism for over 60 years with a status as the most studied prokaryotic organism. With its fast growth...low cost, it’s understandable why E. coli is the most popular bacterial organism in microbiology and biochemistry...developed to thrive in laboratory settings. One of its most attractive features is its stability and easily ...Youn, 2016). For these reasons rats were once the most used mammalian model in medical research with an...has its perks, it is far from perfect. One of the most limiting attributes of rats is their difficult to...

  19. Technical Design of a Western Blot

    Type
    Blog Post
    ...assay. The most common options are 4 °C overnight or 1–2 hours at room temperature (RT). Most people choose...need depends mostly on the weight of the proteins you’re interested in. Table 1 lists the most common types...wet transfer is the most efficient method, meaning that it will transfer the most protein from your gel...probably the most limiting of steps, as you may only have access to one or another. Most institutes have...blot protocol and protocol video!  Antibodies The most important part of your western is your antibodies...direct detection method Indirect detection is the most common way to run a western blot. It allows you ...different lysis buffers available commercially, and most common buffers have recipes available online if ...

  20. Viral Vectors 101: Systemic Capsids

    Type
    Blog Post
    ...vasculature (adult marmosets), biased toward astrocytes (adult rhesus macaque) Rats, marmosets, green monkeys... including ENS (adult marmosets, infant rhesus macaques) Mice, rats, marmosets, rhesus macaques Unknown... screened in marmosets. AAV.CAP-Mac effectively transduces vasculature in adult marmosets (Chuapoco et...transduction levels in marmoset brain, with both neurons and astrocytes targeted. While marmoset liver transduction...capsids for your research? While systemic capsids mostly function like your average AAVs — and have many...accommodate larger, modular genetic payloads. Since most systemic vectors are primarily derived from AAV9...
Showing: 81 - 100 of 764 results