We narrowed to 143 results for: Mycs

1. Tag Your Favorite Yeast Genes with Ease

   Type

   Blog Post

   ...and tagging at either the N- or C-terminus (3xHA, 13xMyc, GST, or GFP). Longtine et al.(3) describe a complimentary...

2. What's New in CRISPR - March 2020

   Type

   Blog Post

   ...that controls repression of gene expression in Saccharomyces cerevisiae. The system consists of dCas9, the...

3. pSiM24: Simplifying Plant Genetic Engineering

   Type

   Blog Post

   ...functional in a variety of plant species. The kanamycin antibiotic resistance gene is controlled by a ...

4. What the HEK?

   Type

   Blog Post

   ...cell lines are susceptible to contamination by mycoplasma and bacteria, but HEKs are also particularly ...

5. Plasmids 101: A Brief History of Plasmids and an Improved eBook!

   Type

   Blog Post

   ...resistant plasmid, pSC101, and a newly developed kanamycin resistant plasmid, pSC102, with EcoRI and selected...

6. Rewiring Metabolic Circuitry with CRISPR RNA Scaffolds [Video]

   Type

   Blog Post

   ... various metabolites in Escherichia coli or Saccharomyces cerevisiae with few techniques that are easily...

7. Visualizing Translation at the Single Molecule Level

   Type

   Blog Post

   ...Using translation inhibitors cycloheximide and puromycin, Halstead et al. verified that translation is ...

8. K. phaffii: Rising to the Occasion

   Type

   Blog Post

   ...Resources References Liu, Wei, et al., From Saccharomyces cerevisiae to human: The important gene co‑expression...

9. Four Factors that Differentiate the Stem Cell Field

   Type

   Blog Post

   ...by expressing four proteins Oct4, Sox2, Klf4, and cMyc (the so called OSKM factors). As the Yamanaka lab's...

10. Viral Vectors 101: Gamma-Retroviral Packaging Systems

    Type

    Blog Post

    ... MCS for cloning X gene, an SV40 promoter, and Neomycin selection.   Table 1: Gamma-retrovirus glossary...

11. Protein Tagging with CRISPR/Cas9: A Conversation with Mendenhall and Myers

    Type

    Blog Post

    .... It also gives cells antibiotic resistance to neomycin so that you can select the population of cells...

12. CRISPR 101: Non-Homologous End Joining

    Type

    Blog Post

    ...End-Joining Strategy for Efficient Genome Editing in Mycobacterium tuberculosis. mBio 11: . https://doi.org/10.1128...

13. Hot Plasmids April 2018 - Protein Degradation, Nanoscopy, FIRE-Cas9, and Yeast Expression Tools

    Type

    Blog Post

    ...targeting of mTOR producing similar results to rapamycin inhibition. To assess TRIM21 toxicity, Clift et...

14. Tagging Optogenetics and Chemogenetics Receptors: Fluorescent Proteins and Other Options

    Type

    Blog Post

    ...RAS-responsive adenylyl cyclase complex from Saccharomyces cerevisiae by use of an epitope addition method...

15. Plasmids 101: Cre-lox

    Type

    Blog Post

    ...recombination event in a single system. The Saccharomyces cerevisiae FLP-FRT recombination system is another...

16. Popular Retroviral Vectors and Their Uses in Scientific Research

    Type

    Blog Post

    ...Tyler Jacks. It carries CRE recombinase and a puromycin resistance cassette. No time to produce virus...

17. Simple CRISPR-based Epigenetic Editing: dCas9-directed DNA Demethylation

    Type

    Blog Post

    ...lentiviral backbones expressing blasticidin or puromycin resistance genes.  It’s that simple! Good luck...

18. Making CRISPR Plasmids Using Fragmid

    Type

    Blog Post

    ...Plasmid pRDB_052 (for stable fly cell lines; Hygromycin resistance gene; for genome integration by spontaneous...

19. Hot Plasmids and Viral Preps - March 2021

    Type

    Blog Post

    ...repression, and editing with a single Cas9-VPR in Saccharomyces cerevisiae. To control Cas9 activity and influence...

20. xCas9: Engineering a CRISPR Variant with PAM Flexibility

    Type

    Blog Post

    ...bacteria where cleavage leads to the loss of spectinomycin resistance. xCas9 3.7 showed the broadest PAM...