We narrowed to 142 results for: Mycs;

1. New Podcast Segment: Hot Plasmids

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   ...this kit to complement unused auxotrophies in Saccharomyces strains.  Listen to Our Segment on The Yeast...

2. Tag Your Favorite Yeast Genes with Ease

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   ...and tagging at either the N- or C-terminus (3xHA, 13xMyc, GST, or GFP). Longtine et al.(3) describe a complimentary...

3. What's New in CRISPR - March 2020

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   ...that controls repression of gene expression in Saccharomyces cerevisiae. The system consists of dCas9, the...

4. pSiM24: Simplifying Plant Genetic Engineering

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   ...functional in a variety of plant species. The kanamycin antibiotic resistance gene is controlled by a ...

5. What the HEK?

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   ...cell lines are susceptible to contamination by mycoplasma and bacteria, but HEKs are also particularly ...

6. Plasmids 101: A Brief History of Plasmids and an Improved eBook!

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   ...resistant plasmid, pSC101, and a newly developed kanamycin resistant plasmid, pSC102, with EcoRI and selected...

7. Rewiring Metabolic Circuitry with CRISPR RNA Scaffolds [Video]

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   ... various metabolites in Escherichia coli or Saccharomyces cerevisiae with few techniques that are easily...

8. Visualizing Translation at the Single Molecule Level

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   ...Using translation inhibitors cycloheximide and puromycin, Halstead et al. verified that translation is ...

9. K. phaffii: Rising to the Occasion

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   ...Resources References Liu, Wei, et al., From Saccharomyces cerevisiae to human: The important gene co‑expression...

10. Four Factors that Differentiate the Stem Cell Field

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    ...by expressing four proteins Oct4, Sox2, Klf4, and cMyc (the so called OSKM factors). As the Yamanaka lab's...

11. Viral Vectors 101: Gamma-Retroviral Packaging Systems

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    ... MCS for cloning X gene, an SV40 promoter, and Neomycin selection.   Table 1: Gamma-retrovirus glossary...

12. Protein Tagging with CRISPR/Cas9: A Conversation with Mendenhall and Myers

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    .... It also gives cells antibiotic resistance to neomycin so that you can select the population of cells...

13. CRISPR 101: Non-Homologous End Joining

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    ...End-Joining Strategy for Efficient Genome Editing in Mycobacterium tuberculosis. mBio 11: . https://doi.org/10.1128...

14. Hot Plasmids April 2018 - Protein Degradation, Nanoscopy, FIRE-Cas9, and Yeast Expression Tools

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    ...targeting of mTOR producing similar results to rapamycin inhibition. To assess TRIM21 toxicity, Clift et...

15. Tagging Optogenetics and Chemogenetics Receptors: Fluorescent Proteins and Other Options

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    ...RAS-responsive adenylyl cyclase complex from Saccharomyces cerevisiae by use of an epitope addition method...

16. Plasmids 101: Cre-lox

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    ...recombination event in a single system. The Saccharomyces cerevisiae FLP-FRT recombination system is another...

17. Popular Retroviral Vectors and Their Uses in Scientific Research

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    ...Tyler Jacks. It carries CRE recombinase and a puromycin resistance cassette. No time to produce virus...

18. Simple CRISPR-based Epigenetic Editing: dCas9-directed DNA Demethylation

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    ...lentiviral backbones expressing blasticidin or puromycin resistance genes.  It’s that simple! Good luck...

19. Making CRISPR Plasmids Using Fragmid

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    ...Plasmid pRDB_052 (for stable fly cell lines; Hygromycin resistance gene; for genome integration by spontaneous...

20. Hot Plasmids and Viral Preps - March 2021

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    ...repression, and editing with a single Cas9-VPR in Saccharomyces cerevisiae. To control Cas9 activity and influence...