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We narrowed to 400 results for: NAM

Showing: 81 - 100 of 400 results
  1. 22 Hot Plasmid Technologies from 2014

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    Blog Post
    ... in the cephalochordate Branchiostoma floridae, named bfloGFPs. They went on to characterize the structural...CoChR), and Scherffelia dubia (SdChR). ShChR, nicknamed Chronos, is blue and green light-excitable and...than those of other channelrhodopsins. CnChR1, nicknamed Chrimson, is the first reported yellow-peaked ...0.4ms from 21.4 ± 1.1 ms); this new variant was named ChrimsonR. Plasmids containing Chronos, Chrimson... overexpression or knock-down, where signaling dynamics are influenced by the relatively long life-cycle...assembly of plant transformation constructs. As the name suggests, GreenGate is based on the Golden Gate ...
  2. How to Make Friends and Meet People at a Scientific Conference

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    Blog Post
    ...handy and make sure your name tag is always visible. Some people learn names by seeing them. It is OK ...level. Right away you have something in common. Namely, the scientific question you are interested in ...OK to ask someone to see their name tag if you are one of those people. Meet people in sessions by sitting...
  3. Deep Dive: qPCR

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    Blog Post
    ...probably know as TaqMan) and dsDNA dyes (commercial name: Sybr). However, there are other chemistries available...online tool that uses the  amplicon sequence and thermodynamic and experimental parameters (neighbor stacking...temperature range) to predict PCR melting curves and dynamic melting profiles (Dwight, 2011).  Reference Genes...prediction of high-resolution melting curves and dynamic melting profiles of PCR products in a rich web ...11237753. Primer design resources PrimerBlastDINAmelMfoldBLASTEnsembl More resources on the Addgene blog...
  4. New Tool for Lineage Tracing: The ClonTracer Library

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    Blog Post
    ...infection and to monitor their abundance and clonal dynamics over time using next generation sequencing (NGS...the ClonTracer library and monitored population dynamics after treatment with EGFR inhibitor, erlotinib...using the ClonTracer library to monitor clonal dynamics following drug treatment in cell line and primary... 1. Hyo-eun, C. Bhang, et al. "Studying clonal dynamics in response to cancer therapy using high-complexity...
  5. Zhang Lab CRISPR Page

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    Collection
    ...mice, Platt et al . simultaneously modeled the dynamics of KRAS , p53 and LKB1 , the top three significantly...
  6. Hot Plasmids: Summer 2025

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    Blog Post
    ...components) and in a FRET-based biosensor for cAMP dynamics. The mGold2 variants significantly enhanced photostability...Alyssa Shepard While CRISPR has become a household name, the search for smaller genome editors is heating...TRV), a bipartite RNA virus, to deliver a TnpB (named ISYmu1) to the popular plant model Arabidopsis (...heterogeneity of hematopoietic stem cells and track clonal dynamics during differentiation after they introduced mutations...
  7. Hot Plasmids: Spring 2025

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    Blog Post
    ...antibodies for Glypicans DNA editing with STITCHR Dynamic protein labeling with TurboCas Enhancer-AAV viral...combined CRISPR / retrotransposon system with the pithy name "site-specific target-primed insertion through targeted...025-08877-4.  Two powerhouse tools combine for dynamic protein labeling By Alyssa Shepard To fully understand... the genomic targeting power of dCas9 with the dynamic labeling power of miniTurboID, a proximity ligation...
  8. Which Fluorescence Microscopy Technique is Best for Me?

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    Blog Post
    ...start by asking two questions: 1) Is the sample dynamic or static? 2) Is the sample thin (< 15 μm) or thick...day fluorescence microscopes (see Table 1). Thin dynamic samples Ex: monolayers of live cells with fluorescently...as a scientific CMOS camera. If plasma membrane dynamics are the main focus of the experiment, a Total ...in resolution, down to 10s of nanometers. Thick dynamic samples Ex: 3D cell cultures, small model embryos...
  9. Special Delivery: Fluorophore Targeting for FRET Studies

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    Blog Post
    ...control experiments are needed to establish the dynamic range of your assay. These experiments include ...values you can expect, thereby establishing the dynamic range of your FRET assay (Figure 2). These labeling...PMCID: PMC2735349. 8. Miyawaki, Atsushi, et al. "Dynamic and quantitative Ca2+ measurements using improved...Titov, and Jin Zhang. "Analyzing protein kinase dynamics in living cells with FRET reporters." Methods ...
  10. Your Top Requested Plasmid in 2016!

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    Blog Post
    ...PX459) V2.0: What's not in the name? Not included in this plasmid's name are some additional features. ...CRISPR nuclease, SpCas9 from the Zhang Lab. It's name, though a little confusing to the uninitiated, contains...
  11. Mapping the 4D nucleome with CRISPR/Cas9

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    Blog Post
    ...gRNA, facilitating the live study of chromatin dynamics at a particular locus. A limitation of this system...used to examine both inter- and intrachromosomal dynamics, and how they change in response to the cell cycle...the plasmids from this publication at Addgene. Dynamic imaging of genomic loci in living human cells by...
  12. Cellosaurus: A Cell Line Information Database

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    Blog Post
    ...Cellosaurus is searchable by cell line name (recommended names and synonyms will all get you to the right...an 'h' or a 'b' written on the flask (and if the name was the correct one!)    Fig. 1: HeLa cells...
  13. Visualizing Translation at the Single Molecule Level

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    Blog Post
    ...this single mRNA; you get a view of translational dynamics at the single molecule level. The coding sequence...methods increase our understanding of translation dynamics, but they come with a few caveats. In both cases...this paper at Addgene. 3. Yan, Xiaowei, et al. “Dynamics of Translation of Single mRNA Molecules In Vivo...
  14. 5 Great Apps for Lab Life

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    Blog Post
    ...to write your colleagues’ names in a polypeptide chain, except if their names have a B, J, X, or Z. Some...user can search by sequence, category of enzyme, or name to discover all the information about that restriction...
  15. 15 Hot Plasmids from 2017

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    Blog Post
    ...precise genome modification. Plasmid ID Plasmid Name Cas9 Variant Speacial Features 85169  pJL-SaBE3... the B. subtilis Single Gene Deletion Library—Kanamycin and the CRISPRi Essential gene Knockdown library...regions (excluding start and stop codons) with kanamycin cassettes and were carefully designed to allow... a Kruppel-like zinc finger protein (previously named ZBTB48) in regulating the upper-bound of telomere...
  16. From Lab Bench to Leadership: Transitioning into Management as a PhD

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    Blog Post
    ...provide timely feedback, leading to unproductive dynamics. I experienced this firsthand when it took me ...and online courses focused on leadership, team dynamics, and project management. Mentorship is another...or DISC assessments can help you navigate team dynamics effectively and foster a cohesive, productive ...
Showing: 81 - 100 of 400 results