...mice, Platt et al . simultaneously modeled the dynamics of KRAS , p53 and LKB1 , the top three significantly...

... intensity and wavelength can be used to study dynamic processes. Conversely, varied input patterns may... activity persisted in the inactive state. The dynamic range, defined as the difference between the lowest...They also reduced system leakiness and increased dynamic range to around 100-fold. These improvements will...system. Applications of these systems The large dynamic range of these systems ensures that they can be...

... in the cephalochordate Branchiostoma floridae, named bfloGFPs. They went on to characterize the structural...CoChR), and Scherffelia dubia (SdChR). ShChR, nicknamed Chronos, is blue and green light-excitable and...than those of other channelrhodopsins. CnChR1, nicknamed Chrimson, is the first reported yellow-peaked ...0.4ms from 21.4 ± 1.1 ms); this new variant was named ChrimsonR. Plasmids containing Chronos, Chrimson... overexpression or knock-down, where signaling dynamics are influenced by the relatively long life-cycle...assembly of plant transformation constructs. As the name suggests, GreenGate is based on the Golden Gate ...

...handy and make sure your name tag is always visible. Some people learn names by seeing them. It is OK ...level. Right away you have something in common. Namely, the scientific question you are interested in ...OK to ask someone to see their name tag if you are one of those people. Meet people in sessions by sitting...

...probably know as TaqMan) and dsDNA dyes (commercial name: Sybr). However, there are other chemistries available...online tool that uses the  amplicon sequence and thermodynamic and experimental parameters (neighbor stacking...temperature range) to predict PCR melting curves and dynamic melting profiles (Dwight, 2011).  Reference Genes...prediction of high-resolution melting curves and dynamic melting profiles of PCR products in a rich web ...11237753. Primer design resources PrimerBlastDINAmelMfoldBLASTEnsembl More resources on the Addgene blog...

...infection and to monitor their abundance and clonal dynamics over time using next generation sequencing (NGS...the ClonTracer library and monitored population dynamics after treatment with EGFR inhibitor, erlotinib...using the ClonTracer library to monitor clonal dynamics following drug treatment in cell line and primary... 1. Hyo-eun, C. Bhang, et al. "Studying clonal dynamics in response to cancer therapy using high-complexity...

...Subpool Viral Prep Available Yes No Clear Filters Name ID Library Type Species PI Lentiviral Generation...

... photons. The robust signal, sensitivity, wide dynamic range, and resistance to photobleaching has made...

... AAV8 AAV9 Retrograde AAV PHPeB Clear Filters ID Name Promoter Sensor Label Activity Serotype PI Acetylcholine...

...antibodies for Glypicans DNA editing with STITCHR Dynamic protein labeling with TurboCas Enhancer-AAV viral...combined CRISPR / retrotransposon system with the pithy name "site-specific target-primed insertion through targeted...025-08877-4.  Two powerhouse tools combine for dynamic protein labeling By Alyssa Shepard To fully understand... the genomic targeting power of dCas9 with the dynamic labeling power of miniTurboID, a proximity ligation...

...start by asking two questions: 1) Is the sample dynamic or static? 2) Is the sample thin (< 15 μm) or thick...day fluorescence microscopes (see Table 1). Thin dynamic samples Ex: monolayers of live cells with fluorescently...as a scientific CMOS camera. If plasma membrane dynamics are the main focus of the experiment, a Total ...in resolution, down to 10s of nanometers. Thick dynamic samples Ex: 3D cell cultures, small model embryos...

...control experiments are needed to establish the dynamic range of your assay. These experiments include ...values you can expect, thereby establishing the dynamic range of your FRET assay (Figure 2). These labeling...PMCID: PMC2735349. 8. Miyawaki, Atsushi, et al. "Dynamic and quantitative Ca2+ measurements using improved...Titov, and Jin Zhang. "Analyzing protein kinase dynamics in living cells with FRET reporters." Methods ...

... top Secretory Pathway The secretory pathway is named for its role in secreting proteins from the cell...

...PX459) V2.0: What's not in the name? Not included in this plasmid's name are some additional features. ...CRISPR nuclease, SpCas9 from the Zhang Lab. It's name, though a little confusing to the uninitiated, contains...

...gRNA, facilitating the live study of chromatin dynamics at a particular locus. A limitation of this system...used to examine both inter- and intrachromosomal dynamics, and how they change in response to the cell cycle...the plasmids from this publication at Addgene. Dynamic imaging of genomic loci in living human cells by...

...Cellosaurus is searchable by cell line name (recommended names and synonyms will all get you to the right...an 'h' or a 'b' written on the flask (and if the name was the correct one!)    Fig. 1: HeLa cells...

...backbone contains a kanamycin resistance cassette, you would plate the cells on kanamycin-containing agar..... Only cells carrying plasmids that contain a kanamycin resistance cassette will grow. However, often ...

...microtubule-associated protein to regulate cell growth dynamics Article contributed by Klaus Wanisch  Listen to...Torsten Wittmann’s lab at UCSF to modulate cellular dynamics with light by modifying EB1. EB1 normally sits...novel ways in manipulating and studying cellular dynamics. The photoinducible control of protein function...

...this single mRNA; you get a view of translational dynamics at the single molecule level. The coding sequence...methods increase our understanding of translation dynamics, but they come with a few caveats. In both cases...this paper at Addgene. 3. Yan, Xiaowei, et al. “Dynamics of Translation of Single mRNA Molecules In Vivo...

...recent blog post. Fatima Enam: Biosensors for human milk oligosaccharides Fatima Enam is currently a graduate...Commemorative Travel Awardees: Xin Zhou and Fatima Enam. There were many excellent applicants but Xin and...