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Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 416 results for: Numb

Showing: 81 - 100 of 416 results

  1. Plasmid Tools for Microbiome Studies

    Type
    Blog Post
    ...too costly and time intensive to process a large number of microbiome samples. To enable large-scale, longitudinal...Toolkit by replacing the backbone vector’s high-copy number bacterial ColE1 origin with the RSF1010 origin,...particle exponentially decreases over time and the number of elapsed generations can be determined. The lab...

  2. Summer SciComm: Preprints

    Type
    Blog Post
    ...applications. A number of organizations (such as ASAPbio) advocate for preprints, and a growing number of funding...is valuable, modern scientific publishing has a number of drawbacks, especially the increasingly growing...

  3. Choosing the B(right)est Fluorescent Protein: Photostability

    Type
    Blog Post
    ...be excited many times, thereby producing a large number of emitted photons before they are destroyed. ...decreasing the power of the excitation light reduces the number of excitation/emission cycles per unit of time,... are performed in different ways. We describe a number of issues with some of the experimental designs...

  4. Addgene's Tips for Plasmid Quality Control

    Type
    Blog Post
    ...least twice, and sometimes more depending on the number of features we need to verify. Determining which...is critical for the efficient handling of large numbers of samples. When we grow overnight cultures for...submission instructions. As for the analysis of a large number of sequencing reactions, you can make your life...

  5. Adenoviral Vector Production and Troubleshooting

    Type
    Blog Post
    ...calculate the particle number: OD260 reading x dilution factor x 1.1 x 1012 particles = number of particles per...purified virus should be ~1.3 Quantitative PCR: the number of viral DNA packaged in virions is determined ...

  6. Tips for Technical Support Calls

    Type
    Blog Post
    ...catalog number, or at least the plasmid name in your initial query. This can reduce the number of messages...questions about an Abcam antibody. Have your catalog number ready. I've attempted to answer several emails ...

  7. Tips for Using BLAST to Verify Plasmids

    Type
    Blog Post
    ... years (we now have over 60,000 plasmids!), the number of sequencing results we analyze as part of our...sequences (nr)” database as it contains the largest number of protein sequences. Just below the BLAST button...isoforms. In addition to our favorites, there are a number of sequence alignment tools available. Try these...

  8. Great Results Start with Great Standard Curves

    Type
    Blog Post
    ...western blot. Standard curves are critical for a number of popular scientific applications, and the quality... is because standard curves vary depending on a number of factors, including the user, equipment, assay...accurately measure.  Dynamic range depends on a number of factors, including the dilution series of the...

  10. The Effect of COVID-19 on Liu Lab Plasmid Requests From Addgene Data

    Type
    Blog Post
    ... requests by date. Parentheses depict the total number of plasmids ordered for each paper. Rough estimation...affected by the COVID-19 pandemic, we compared the number of plasmid orders and new COVID-19 cases for each... our data: We choose to let yx(t) represent the number of unique PIs or labs placing orders for paper ...

  11. A Guide to Starting Your Own Journal Club

    Type
    Blog Post
    ... Our scheduling was in part defined by both the number of participants and the standard academic calendar...logistics Once you decide on the topic, there are a number of administrative and practical decisions to make...scheduling the second and other subsequent meetings. The number of participants, breadth of the discussed topic...

  12. AAVs CREATed for Gene Delivery to the CNS and PNS

    Type
    Blog Post
    ...were derived from. They also transduced a higher number of neurons overall (GFP+ cells that also stained...doses of virus are used in order to decrease the number of neurons that get labeled, neurons are more likely...tTA), is injected at a high dose to maximize the number of cells that are labeled. Part 2: An AAV expressing...

  14. Plasmids 101: Protein tags

    Type
    Blog Post
    ...to overexpress and purify proteins can face any number of technical challenges depending on their protein...might consider junk protein. Though there are a number of chemical and peptide-based proteosome inhibitors...of this cleavage depends on the identity of X. A number of other proteases are available, but scientists...

  15. Viral Vectors 101: Preparing Pooled Libraries

    Type
    Blog Post
    ...cells than the number of plasmids in the library.  Spinfection If you have a large number of cells to transduce...lentiviral preps are generally aliquoted to reduce the number of freeze-thaw cycles needed when using the virus...

  16. What's Your Organism? Expanding Genomic Tools via the NSF EDGE Program

    Type
    Blog Post
    ...sharing The EDGE Program is designed to expand the number of systems that can be studied by providing support...Rosenthal) The Hawaiian bobtail squid has an extreme number of nerve cells and an unusually high level of RNA...homologous recombination efficiency to reduce the number of plants you need to grow to get a recombinant...

  17. Plasmids 101: Broad Host Range Plasmids

    Type
    Blog Post
    ...proteins. These iterons vary in their sequence and number and can alter the efficiency of replication in ...Regulatory elements within the ori also control copy number and stability, which influence the plasmid’s ability...self-transmissible plasmids may have high copy numbers and reduced metabolic load (Meyer 2009). ...

  19. Using Video to Share Your Science: We Share Science

    Type
    Blog Post
    ...journals Current Biology (2,500 views), Journal of Number Theory (1,519 views), and Gastroenterology (1,046...., learn why water freezes) there was a growing number of online research abstracts. With journals in ...Noorden, Richard. "Interdisciplinary research by the numbers." Nature 525.7569 (2015): 306-307. 3. Spicer, Scott...

  20. 5 Reasons to Use Reddit for Science Communication

    Type
    Blog Post
    ...advised me on how to alter my intro to reduce the number of potentially hostile posters (this advice included...moderators did not delete or suppress were the large number of scientist posters with serious career dilemmas...associated with my LinkedIn profile. There are a number of ways you can build up your own expertise and...
Showing: 81 - 100 of 416 results