We narrowed to 131 results for: PTS

1. PhD Applications After COVID

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   Blog Post

   ...lab, research, and provide them with a CV or transcripts if requested Thank them, and eagerly await a ...

2. AAV Vector Quality Control: Going the Extra Mile with NGS

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   Blog Post

   ...identification, we developed custom open-access Python scripts that can perform a deeper analysis of the data....

3. Plasmids 101: Optimizing Plasmid Yields

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   Blog Post

   ...concentration Low copy plasmids produce fewer transcripts of their antibiotic resistance genes and can ...

4. Experimenting with New Careers while in Grad School

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   Blog Post

   ...gained a concrete example to use to promote future attempts and learned a bit about recruiting scientists ...

5. Plasmids 101: Multicistronic Vectors

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   Blog Post

   ...Unlike promoters which will create unique mRNA transcripts for each gene that is expressed, multicistronic...

6. Interview: Nicola Patron on Plant Synthetic Biology, MoClo, and More

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   Blog Post

   ...well in plants and give similar stability to transcripts as the ones that people use all the time. Now...

7. Negative Can Be Positive: Open AAV Data with Addgene

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   Blog Post

   ...’ results is available not through published manuscripts, but through word of mouth or old lab notebooks...

8. CRISPR 101: RNA Editing with Cas13

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   Blog Post

   ...the RESCUE system’s ability to edit endogenous transcripts finding up to 42% editing efficiency. They note...

9. Capturing a Moment with Marc Zimmer’s “The State of Science”

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   Blog Post

   ...and communicating science for over two decades, attempts to answer it in five paragraphs, with examples...

10. SciComm: Taking Science to Elementary and Middle Schools

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    Blog Post

    ...analogy that would help them grasp some of the key concepts (Fig. 2). One thing I will likely change for next...

11. DNA Purification Without a Kit

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    Blog Post

    ...isothiocyanate for the gel extraction protocol) disrupts the DNA’s hydrogen bonds with water molecules ...

12. Lentiviral Vector Uses and Overview

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    Blog Post

    ... LTR) and Rev (facilitates nuclear export of transcripts). A different viral envelope protein (Env), usually...

13. QC Sequencing Technologies at Addgene

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    Blog Post

    ...plasmid sequence, it moves to the next. If all three attempts fail, the QC team manually checks and assembles...

14. Tips and Tricks for Using Golden Gate Modular Cloning (MoClo)

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    Blog Post

    ...Careful use of your glycerol stocks When your lab adopts the MoClo technology, you will quickly go through...

15. Viral Vectors 101: Preparing Pooled Libraries

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    Blog Post

    ...the library sample, and any other information or scripts provided by the depositing lab for data analysis...

16. Overcoming the AAV Size Limitation for CRISPR Delivery

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    Blog Post

    ...into one AAV, Cas9 must be made smaller. Previous attempts to “shrink” Cas9 include the use of St1Cas9 (~...

17. Four Base Editing Reporters to Monitor and Enrich Editing in Real-time

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    Blog Post

    ...the lab introduced a 43 base-pair insertion that disrupts fluorescence due to a frameshift. Restoration ...

18. Deciphering the Mysteries of Behavior with Viral Vectors

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    Blog Post

    ...opposing behaviors are possible: active swimming in attempts to escape, or passive floating on the water surface...

19. Save Time with Transient Plant Leaf Transformations

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    Blog Post

    ...use of introns, which will be removed from the transcripts in the plant but not from Agrobacterium. Test...

20. CRISPR-mediated Plant Base Editors

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    Blog Post

    ...rise to a mutant TadA (TadA*) that satisfactorily accepts DNA as its substrate (see figure 2).          The...