We narrowed to 129 results for: Pts

1. Viral Vectors 101: Voltage Indicators

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   Blog Post

   ...the other GEVI systems that use engineered FPs. Attempts have been made to optimize their fluorescence,...

2. PhD Applications After COVID

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   Blog Post

   ...lab, research, and provide them with a CV or transcripts if requested Thank them, and eagerly await a ...

3. Plasmids 101: Optimizing Plasmid Yields

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   ...concentration Low copy plasmids produce fewer transcripts of their antibiotic resistance genes and can ...

4. Experimenting with New Careers while in Grad School

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   ...gained a concrete example to use to promote future attempts and learned a bit about recruiting scientists ...

5. Plasmids 101: Multicistronic Vectors

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   ...Unlike promoters which will create unique mRNA transcripts for each gene that is expressed, multicistronic...

6. Interview: Nicola Patron on Plant Synthetic Biology, MoClo, and More

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   Blog Post

   ...well in plants and give similar stability to transcripts as the ones that people use all the time. Now...

7. CRISPR 101: RNA Editing with Cas13

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   Blog Post

   ...the RESCUE system’s ability to edit endogenous transcripts finding up to 42% editing efficiency. They note...

8. Capturing a Moment with Marc Zimmer’s “The State of Science”

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   ...and communicating science for over two decades, attempts to answer it in five paragraphs, with examples...

9. SciComm: Taking Science to Elementary and Middle Schools

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   Blog Post

   ...analogy that would help them grasp some of the key concepts (Fig. 2). One thing I will likely change for next...

10. Negative Can Be Positive: Open AAV Data with Addgene

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    Blog Post

    ...’ results is available not through published manuscripts, but through word of mouth or old lab notebooks...

11. DNA Purification Without a Kit

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    Blog Post

    ...isothiocyanate for the gel extraction protocol) disrupts the DNA’s hydrogen bonds with water molecules ...

12. Lentiviral Vector Uses and Overview

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    ... LTR) and Rev (facilitates nuclear export of transcripts). A different viral envelope protein (Env), usually...

13. Overcoming the AAV Size Limitation for CRISPR Delivery

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    ...into one AAV, Cas9 must be made smaller. Previous attempts to “shrink” Cas9 include the use of St1Cas9 (~...

14. Four Base Editing Reporters to Monitor and Enrich Editing in Real-time

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    Blog Post

    ...the lab introduced a 43 base-pair insertion that disrupts fluorescence due to a frameshift. Restoration ...

15. Plasmids 101: Plasmid Incompatibility

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    ...bacterial cloning glory, scientists have applied these concepts to deal with the more sinister plasmids of the...

16. Deciphering the Mysteries of Behavior with Viral Vectors

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    Blog Post

    ...opposing behaviors are possible: active swimming in attempts to escape, or passive floating on the water surface...

17. Save Time with Transient Plant Leaf Transformations

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    Blog Post

    ...use of introns, which will be removed from the transcripts in the plant but not from Agrobacterium. Test...

18. CRISPR-mediated Plant Base Editors

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    Blog Post

    ...rise to a mutant TadA (TadA*) that satisfactorily accepts DNA as its substrate (see figure 2).          The...

19. CRISPR 101: Targeting RNA with Cas13a (C2c2)

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    Blog Post

    ...sequence as specified by the crRNA sequence, it adopts an enzymatically “active” state rather than reverting...

20. How to Write a Scientific Review Article

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    Blog Post

    ...Resources on the Addgene Blog Writing Scientific Manuscripts: Literature Searching, Reading, & Organizing ...