We narrowed to 132 results for: Pts

...identification, we developed custom open-access Python scripts that can perform a deeper analysis of the data....

...concentration Low copy plasmids produce fewer transcripts of their antibiotic resistance genes and can ...

...gained a concrete example to use to promote future attempts and learned a bit about recruiting scientists ...

...Unlike promoters which will create unique mRNA transcripts for each gene that is expressed, multicistronic...

...well in plants and give similar stability to transcripts as the ones that people use all the time. Now...

...’ results is available not through published manuscripts, but through word of mouth or old lab notebooks...

...the RESCUE system’s ability to edit endogenous transcripts finding up to 42% editing efficiency. They note...

...and communicating science for over two decades, attempts to answer it in five paragraphs, with examples...

...isothiocyanate for the gel extraction protocol) disrupts the DNA’s hydrogen bonds with water molecules ...

...analogy that would help them grasp some of the key concepts (Fig. 2). One thing I will likely change for next...

... LTR) and Rev (facilitates nuclear export of transcripts). A different viral envelope protein (Env), usually...

...the library sample, and any other information or scripts provided by the depositing lab for data analysis...

...plasmid sequence, it moves to the next. If all three attempts fail, the QC team manually checks and assembles...

...Careful use of your glycerol stocks When your lab adopts the MoClo technology, you will quickly go through...

...development. We’ve shared what we’ve learned, through manuscripts (Ersing et al., 2023; Haery et al., 2019; McDade...

...into one AAV, Cas9 must be made smaller. Previous attempts to “shrink” Cas9 include the use of St1Cas9 (~...

...the lab introduced a 43 base-pair insertion that disrupts fluorescence due to a frameshift. Restoration ...

...bacterial cloning glory, scientists have applied these concepts to deal with the more sinister plasmids of the...

...I would have done. And finally the stakeholder accepts the new feature as done and we’re onto the next...

...of several layers of support: A front-line team accepts any incoming requests and answers questions that...