...In mammalian cells such as stem cells, HDR is relatively rare and DSBs are predominantly repaired by NHEJ...characterize this phenomenon and found a general relationship between the distance of a mutation from the ...(and let them cut close).   Figure 2: Relationship between cut site to mutation distance and editing...mutations When thinking further about the distance relationship described above, we realized that, in addition...incorporation efficiency, we might exploit this relationship to direct the zygosity of the cells. This was...

...variation. Confirmation of sequence can be done from relatively cheap plasmid sequencing. While rAbs are monoclonal...to run a western blot. It allows you to detect relatively low levels of antigen, while providing flexibility...assay design. Conjugated secondary antibodies are relatively cheap and easy to source, making it easy to switch... not available in a pure form, you can perform relative quantification, which measures protein amounts...particular antibody, this truly does provide a relative, not absolute, readout. Normalization Overall ...purified protein not always available Calculate relative quantification Normalizing to ubiquitously expressed...

...and their successes. Although all mentorship relationships are valuable and highly recommended, a mentor...mentor creates a closer and more approachable relationship for the mentee, where (s)he feels truly understood..., mentors and mentees often developed close relationships with a level of sympathy that is difficult to...confidence. These improvements, combined with the relationships fostered through the IMP have motivated many...-Harvard Medical School, where she studies age-related macular degeneration. She is also a blogger who...

...your target then you would expect to see good correlation between the levels of target detected in both...both methods. These approaches have relatively low sensitivity, which can be problematic if you are trying...also influence antibody binding by altering the relative concentration of potential antibody binders and... targeting a different epitope, and look for correlation between the results. Using antibodies against...cytometry using both antibodies, as well as a third unrelated antibody as a negative control (A). The two antibodies...

...particles or relatively short expression times, where as others, such as hSynapsin, are relatively weak and...sites and the open reading frame is inverted in relation to the promoter, hence the name, double-floxed...homologous pairs, and that the degree of homology is correlated with the level of spontaneous recombination. ...this study were packaged at Addgene and had a relatively low recombination rate (0.1% and 0.13% respectively...

...brightness for a few commonly used fluorophores in relation to the laser type. Table adapted from: https:/...are absorbed and then emitted, which creates a relatively stronger — or brighter — signal.   Figure...use brighter fluorophores for markers that are relatively lower in abundance. In contrast, when you expect...again. In the beginning, we talked about the relationship between a photon's wavelength and energy, how... into different levels of brightness and their relation to the fluorophore's excitation efficiency, and...

...Expanding your network of relationships early and often is the most effective tactic a scientist in training...result of existing professional (and personal) relationships. However, most job seekers will and should apply...after the academic bench. Utilizing existing relationships as part of the job application process can dramatically...you can use alumni databases, friends, and even relatives to network your way to a hiring manager. Learn...

...seeds, DNA and other resources for Arabidopsis and related species. Located at The Ohio State University in...member of the Brassicaceae family, Arabidopsis is related to a variety of food crops such as broccoli, cabbage...utility to science, and its well-known edible relatives, it is fair to say that most people do not recognize...Arabidopsis is small, has a short life cycle, and is relatively easy to grow, it is an ideal organism for teachers...

...slight or large variations depending on how closely related these species are. If a gene was moved exactly ...exactly as is from one organism to a distantly related organism, the gene may not express well because of these...saw that a percent sequence identity below 85% correlates with synthetic sequences. The researchers then...growing trend of sourcing genes from distantly related organisms. In 2006, less than 1% of the genes deposited...

... of the time because of relationships. It’s much harder to build relationships when you are more desperate...experiences to share. The conversation builds a relationship for the future and the 1:1 format is good for...you will make good progress on both building relationships and thinking about future career options. 5....

...using simple web forms) while also maintaining a relatively strict database design (schema).  Registered ...allows for visual comparison of FPs and how they relate to one another. .  In addition, the FPbase Fluorescence... page with all the information in the database relating to that specific protein (for instance: EGFP)....retrieve all of the information in the database relating to that set of proteins.  For computer-savvy users...

... Sensitivity Cas9 sensitivity is often closely related to specificity. Sensitivity is a measure of the...the enzymes showed decreased off-target activity relative to Cas9. Since then, the Joung and Jennifer Doudna...showed decreased or similar off-target activity, relative to eSpCas9 and SpCas9-HF across a number of different...research due to its small size and specificity relative to Cas9. As scientists continue to characterize...

...participants in skills useful for future mentoring relationships.   If you would like to start a group mentoring...for the time commitment of a formal mentoring relationship or there aren’t enough senior mentors available...ideas on what to do once you are in a mentoring relationship.  Celebrate your successes and support each... come. Are you involved in Peer Mentoring relationships? Comment below with ideas from your own mentoring...

...is the question. When presenting on building relationships (also known as “networking”), one of the most...networking to start, build, and track professional relationships. Here are my seven best tips beyond the basics...on LinkedIn!” resulting in just a little more relationship. Pro tip: Once you are connected with someone...connection requests LinkedIn is a place to build relationships. If you don’t add a personal note, you waste...

...highlight four tools for finding journal articles related to your actual interests while also staying on ...these types of accounts can help you find papers related to a particular topic or that were published by...tweet about papers too. You can follow journals related to your field of research or larger journals that... on a paper in your feed you can see suggested related researchers, journals, and concepts to help you...

...attending the session, and what is their background relative to your research? Tailor your language to your... you may receive. People often ask about the relationship of your work to recently published papers or...audience just that. Explain what you do know about a related topic, and let them know while you appreciate the...collaborate with a group who presented on a topic related to your research? Find a way to get in contact ...

... one-copy ‘housekeeping’ gene), and quantified relative to a 'normal' sample with known copy number set...nice thing about qPCR is that since it measures relative quantification - that is, since you’re looking...looking at the amount of DNA in your sample relative to your normal control - you can set up your calculations...provides absolute quantitative data, instead of relative quantification.  Even though no one option is ...

... the ultimate resolution can involve ideas not related to the actual data collection task but still be...negotiation skills will help you strengthen relationships with your colleagues and supervisors and lead...negotiation skills are key to maintaining and growing relationships with your colleagues within and outside of your... what you want and can also strengthen your relationships with others who know that working with you will...

...could be because there is no correlation or it could mean that the relationship is non-linear and you should...of determination, R2. R2 is the square of the correlation between the actual values and the predicate values...fit and 0 indicating that there is no linear relationship between the observed and predicted values. This...

... If you are miserable at home or work, your relationships will suffer in both the short run and the long...agree on before you get enmeshed in a serious relationship. #5 Thou shalt not sweat the small stuff The... make your life easier and to have more time to relax and be with friends and family. #7 Thou shalt create...swim lesson missed can mean a world of mental relaxation. And, I’m not sure I should put this in print...