Antibodies 101: Validation
Type
Blog Post
...your target then you would expect to see good correlation between the levels of target detected in both...both methods.
These approaches have relatively low sensitivity, which can be problematic if you are trying...also influence antibody binding by altering the relative concentration of potential antibody binders and... targeting a different epitope, and look for correlation between the results. Using antibodies against...cytometry using both antibodies, as well as a third unrelated antibody as a negative control (A). The two antibodies...