...within seconds.  Addgene’s old homepage had been relatively the same for quite a while and hadn’t kept pace...

...sequence. In contrast, Dre recombinase, the close relative of Cre, displayed an excision efficiency of only...mammalian genomes: genome-wide distribution and relevance for the efficiency of BAC/PAC recombineering techniques...

...proteins are, which makes mixing and matching a relatively easy proposition, explained Michelle Baird, who...

..., 2005; Deisseroth et al., 2006). Despite its relatively recent development, the history of optogenetics...delivery in the central nervous system due to their relatively stable extra-chromosomally long-term expression... neuronal inputs to induce aversion- or reward-related behaviors. The high spatio-temporal precision and...containing FLEx technology. Read the genOway press release for more information. References Abdallah, Khaled...hippocampal interneurons that express corticotropin‐releasing hormone." Hippocampus 26.1 (2016): 41-53. PubMed...

...and T7E1 have been widely adopted due to their relative simplicity and low cost. The problem with these... detection rates mean the investigator can be relatively certain that their samples don’t have off-target...genotyping, is critical to demonstrating causal relationships between genotype and assayed phenotype. Verifying... for validating edits.  Mismatch cleavage assays rely on pairing between the edited strand and wild-type...limit of detection of ~5%. This means they do not reliably detect editing events that occur in less that ...take into account other elements, including those relating to DNA structure (e.g. epigenetic modification...

...AAV-PHP-B, it can be produced with high viral yields relative to AAV9. At lower viral titers than AAV-PHP.B ...when paired with the ubiquitous CAG promoter, is relatively biased toward L5 and inhibitory neurons and astrocytes...C57BL/6J and BALB/cJ mice through LY6C1, with relatively even distribution of target gene expression (...Spatial transcriptomics shows that its tropism is relatively more biased toward excitatory neurons than AAV-PHP.eB...DNA levels (~25-fold and ~4-fold respectively) relative to AAV9 in the pigtail macaque brain slices. Similarly...BALB/cJ mice, meaning that systemic capsids that rely on LY6A interactions cannot cross the BBB in BALB...not have known homologs in NHPs, and capsids that rely on interactions with those two proteins to cross...

...organisms and cell lines. Although ChIP-seq is a relatively simple and robust protocol it does have limitations...or more transient DNA-protein interactions, the reliance on fixation does increase the chance of false-..., which stands for ‘Cleavage Under Targets and Release Using Nuclease,’ was recently developed by the ...digestion. After MNase digestion, fragments are released from nuclei by a short incubation at 37 °C. These...strong detergent to permeabilize cells rather than to rely on nuclei extraction. They report that this change...

... live animal that can be correlated to behavior, the technique is relatively slow and is not suited to...and concentration of released DA in mouse brain slices Visualization of DA release dynamics during locomotion...Though DA firing patterns (or the frequency of its release) are well understood, the direct effect of DA binding..., enabling detection of individual peaks of DA release.  Using dLight1 sensors in experiments The Tian...following types of data: Report endogenous DA release from a single dopaminergic fiber in mouse brain...stimulus in vivo in transgenic zebrafish Report DA release in live mice over the course of Pavlovian conditioning...

...activity on NAG PAMS and reduced activity on NGG PAMs relative to wild type. A new genome-wide CRISPR knockout...opportunities for investigating genetic mechanisms related to morphogenesis, cell fate specification, and ...the internal control (Fig 1). Because this method relies on the fluorescence ratio between the 2 fluorophores...

... to faithfully produce these proteins and its relatively low expression of native channels.  No matter...

...Meeting – far too long. Holding these meetings in relatively isolated resorts creates a sense of camaraderie...

...transcript 1L - RELB v-rel reticuloendotheliosis viral oncogene homolog B I-REL, IREL RFX5 regulatory ...RASGRP3 RAS guanyl releasing protein 3 (calcium and DAG-regulated) GRP3, KIAA0846 RELA v-rel reticuloendotheliosis...CALDAG-GEFII, MGC129998, MGC129999, RASGRP, V, hRasGRP1 RELA v-rel reticuloendotheliosis viral oncogene homolog ...like beta FIZZ1, FIZZ2, HXCP2, RELM-beta, RELMb, RELMbeta, XCP2 RLN1 relaxin 1 H1, RLXH1, bA12D24.3.1, bA12D24.3.2...polypeptide-related sequence A FLJ60820, MGC111087, PERB11.1 MICB MHC class I polypeptide-related sequence...CST-29 CRH corticotropin releasing hormone CRF CRHR1 corticotropin releasing hormone receptor 1 CRF-R,...ESRRA estrogen-related receptor alpha ERR1, ERRa, ERRalpha, ESRL1, NR3B1 ESRRB estrogen-related receptor beta...

...high Cas9 MOI considering that A549 cells are relatively easy to transduce. I ended up with too many Cas9...on genomic DNA. The surveyor assay itself was relatively easy. Figure 4: Detection of edited BRAF...

...scatter (FSC) and provides information about the relative size of the cell; larger cells produce more FSC...experiment, you would need a way to determine the relative contribution of yellow and orange from FITC and...intensity of events. Since intensity positively correlates with expression, the further the shift of a cell...

...are absorbed and then emitted, which creates a relatively stronger — or brighter — signal.   Figure...use brighter fluorophores for markers that are relatively lower in abundance. In contrast, when you expect...brightness for a few commonly used fluorophores in relation to the laser type. Table adapted from: https:/...again. In the beginning, we talked about the relationship between a photon's wavelength and energy, how... into different levels of brightness and their relation to the fluorophore's excitation efficiency, and...

...negatively correlated with GFP intensity, showing that TRICK is an accurate readout of relative reporter...

...using simple web forms) while also maintaining a relatively strict database design (schema).  Registered ...allows for visual comparison of FPs and how they relate to one another. .  In addition, the FPbase Fluorescence... page with all the information in the database relating to that specific protein (for instance: EGFP)....retrieve all of the information in the database relating to that set of proteins.  For computer-savvy users...

...control elements Coding sequence features are relatively straightforward to define, but for control elements...literature, some of it decades old, to provide reliable feature annotations. Even with these extensive...fluorescent proteins, which often come in closely related versions that have different properties. To prevent...

...potential, GCaMP7s is approximately twice as bright, relative to baseline fluorescence, as the GCaMP7f variant...viral vectors. Here are some of the AAV we have released in the last few months. You can also browse our...testing purposes. To address this, we recently released our serotype testing AAV, which are small (20 ...continue to add more controls, and have recently released these controls: pAAV-hSyn-DIO-EGFP (50457-AAV5...and recombinase-dependent vectors. We recently released several recombinases in various serotypes, and...grow this collection. Of note, we are excited to release a Flp-dependent Cre AAV in AAV9 and AAVrg. See ...

...quantify based on band intensities of your DNA relative to the intensity in the ladder. It’s best to compare...