We narrowed to 221 results for: Rras
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TypeBlog Post...contain a deletion in the 3’ LTR that prevents aberrant activation of nearby genes. These safer vectors..., Cavallesco R, Gillet-Legrand B, Caccavelli L, Sgarra R, Maouche-Chrétien L, Bernaudin F, Girot R, Dorazio...
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Cpf1: A New Tool for CRISPR Genome Editing
TypeBlog Post...Cpf1 multiplexing approach using a single crRNA array to express up to 4 crRNAs. Zetsche et al. also suggest...Gene Editing by CRISPR–Cpf1 Using a Single crRNA Array." Nat Biotechnol. 35.1 (2016): 31-34. PubMed PMID... -
A Career in Grants: How To Become a Grant Professional
TypeBlog Post...makers have moved away from long written proposal narratives. Now they require grant seekers to use online...demand for stronger budgets with clear budget narratives, logic models, established partnerships and collaborations... -
Fluorescent Proteins 101: Green Fluorescent Protein (GFP)
TypeBlog Post... the lab. GFP is used in research across a vast array of biological disciplines and scientists employ ...maturation, and fluorescence. Biosensors: A wide array of GFP-based fluorescent biosensors has been designed... -
Overcoming the AAV Size Limitation for CRISPR Delivery
TypeBlog Post..., Barretto R, Habib N, Hsu PD, Wu X, Jiang W, Marraffini LA, Zhang F (2013) Multiplex Genome Engineering...Garneau JE, Dupuis M-È, Villion M, Romero DA, Barrangou R, Boyaval P, Fremaux C, Horvath P, Magadán AH... -
Finding nucleic acids with SHERLOCK and DETECTR
TypeBlog Post...and portability. This method can be used for an array of applications including clinical diagnostics (...detection of nucleic acids in a mixed population for an array of molecular and clinical diagnostic applications... -
Hot Plasmids Spring 2024
TypeBlog Post...for the indicated ssAAV5s. Figure adapted from Serrano et al. 2023 under a CC-BY-NC-ND license. Integrating... Find microglia-targeting AAV plasmids here! Serrano, C., et al. (2023). Simple and Highly Specific ... -
Plasmids 101: Broad Host Range Plasmids
TypeBlog Post... Rhodopseudomonas spheroides, Salmonella spp., Serratia marcescens, Streptomyces lividans, Synechococcus...Salmonella enteritidis, S. typhimurium, S. ordonez, Serratia marcescens, Shigella spp., Vibrio cholerae, Xanthomonas... -
New Acoustic Reporter Genes: Ultrasound Imaging of Gene Expression
TypeBlog Post...vesicle-expressing species. The gas vesicle genes from Serratia sp. 39006 produced the highest levels of nonlinear... this gene set bARGSer, for bacterial ARG from Serratia sp. 39006. We next tested bARGSer with several... -
CRISPR 101: Which Cas9 Do I Choose for My CRISPR Experiment?
TypeBlog Post...experiment. Here, we will introduce you to the wide array of Cas9s (many available through Addgene’s repository...VP64 along with other SAM factors, and the Suntag array of factors, all targeted to the promoter region.... -
Plasmids 101: Biotinylation
TypeBlog Post...commonly used today in molecular biology for an array of different techniques and protocols. In this post... Thermo Fisher Scientific's guide. There are an array of other molecular biology applications for biotin... -
Evolution of Brainbow: Using Cre-lox for Multicolor Labeling of Neurons
TypeBlog Post...Brainbow provided neuroscientists with the vast array of colors needed to mark individual neurons, the...to mark and follow single cells due to the wide array and high stability of colors. Sanes and Lichtman... -
Deep Dive: Statistical Tests (Comparisons)
TypeBlog Post...identifies a p value cut off. This method entails arranging all calculated p values from smallest to largest...): 126-130 (2012). 10.1097/SMJ.0b013e3182498ad5 Curran-Everett, Douglas. Explorations in statistics: statistical... -
The Codon Guide to Synthetic Biology
TypeBlog Post...contours of each topic. The video channels are also underrated and I recommend checking them out, especially... -
SciComm with the Experts at Science in the News Part 1
TypeBlog Post...Jessica Welch Have Had to Explain Importance of Narrative in Science Communication Conversation in Science... -
Introducing Addgene's Microbiology Pages
TypeBlog Post... constructs, why not let us know? Fill out our referral form and we’ll take care of the rest. Know of... -
Synthetic Biology - Overview
TypeCollection... Anastasios Melis Aindrila Mukhopadhyay Richard Murray Erin O'Shea Martin Parniske Antonio Richart Herbert... -
Introducing an All-in-One CRISPR/Cas9 Vector System for Multiplex Genome Engineering
TypeBlog Post...plan to adapt the new tool for use with a broader array of Cas9s, which they will also make available when... -
6 Tips for Analyzing and Troubleshooting Sanger Sequencing Results
TypeBlog Post...Anything more and you’re venturing into the uncertain terrain. 4. Never trust the first 20-30 bases of a DNA ... -
Keeping Up With CRISPR/Cas9
TypeBlog Post..., in the labs of Chase L. Beisel and Rodolphe Barrangou, had applied the CRISPR/Cas9 system to developing...