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We narrowed to 399 results for: SEF

Showing: 81 - 100 of 399 results

  1. Tips for Using FRET in Your Experiments

    Type
    Blog Post
    ...element found on the neck of my guitar...not really useful for a biologist you would say. The student was ...apprehend molecular complexes and is still a very useful tool.    Like a guitar hero (that I’m not), FRET...appropriate controls are required to make this method useful for dynamic experiments in which FRET changes are...

  2. Antibodies 101: Single Chain Fragment Variables (scFvs)

    Type
    Blog Post
    ...clearance, which is useful for imaging applications; better tissue penetration which is useful for therapeutic...delivery by viral vectors like AAV, which could be useful for delivering scFvs that inhibit HIV.   References...

  3. Five Popular Model Organisms, Part 2

    Type
    Blog Post
    ...making them useful for in vivo studies. These attributes make frog eggs some of the most useful tools in ...2009). Rats are larger than mice making them more useful for certain studies that involve surgery or imaging...

  4. Multiple Plasmids at a Low Price: Kits from Addgene

    Type
    Blog Post
    ...resources,  we’re highlighting just a few of the many useful kits you can order from the repository: FX Cloning...favorite gene at your level of choice and should be useful for a variety of synthetic biology or protein purification...from its creators. We hope you’ll find these kits useful in your research. If you’d ever like to deposit...

  5. 10 Steps to a Perfect Science Talk

    Type
    Blog Post
    ...talks at iBiology which hosts an amazing amount of useful, entertaining science lab, career, and policy content..., you just need to figure out how to make it suspenseful. 4. Start preparing your talk early - like today...information will find it. Experienced speakers present useful protocols, geeky science details, products that...

  6. Viral Vectors 101: AAV Serotypes and Tissue Tropism

    Type
    Blog Post
    ...Tervo et al., 2016). This functionality makes it useful for mapping neural connections. Although derived...can also cross the blood-brain barrier, making it useful for applications targeting the CNS (Issa et al.... tissue tropism, different AAV serotypes can be useful to evade neutralizing antibodies developed from...

  7. Antibodies 101: The Basics of Western Blotting

    Type
    Blog Post
    ...mix derived from cells or tissues. Westerns are useful not only to detect the presence or absence of a...should be repeated.  Finally, while westerns are a useful way to gauge protein abundance they provide qualitative...before and after treatment.  Westerns are also a useful readout for a number of cellular assays. They can...

  8. Technique: Probe Phage Genomes for Host Binding Proteins

    Type
    Blog Post
    ...classes of phage proteins to study. These are highly useful for the biotech industry, which is in the process... gene function. In this post, I will describe a useful and cost-effective method that our lab (the Szymanski... proteases knocked out making them particularly useful for protein production), then dilute and plate ...

  9. PiggyBac-ing Through the Genome Editing Field

    Type
    Blog Post
    ...transposon vectors with us to help us expand this useful resource. While Addgene cannot distribute plasmids... 256 base-pairs of DNA sequence, making it very useful for genetic engineering approaches. Most importantly...expression levels of your gene may be particularly useful when the gene being expressed has threshold effects...

  10. Antibodies 101: Flow Cytometry

    Type
    Blog Post
    ...of scatter is captured by detectors and provides useful information about the cell. Light that continues...of secondary antibodies. It’s also particularly useful for intracellular staining where binding of large...flow cytometry for single cell analysis and some useful background information for setting up antibody ...

  11. Fluorescent Proteins 101: Monitoring Cell Mobility Using Fluorescent Proteins

    Type
    Blog Post
    ...them into appropriate containers. The machine is useful for distinguishing cell populations that have been...green fluorescence to red fluorescence) are also useful tools to use with intravital imaging. These tools...available for tracking certain cell types. Another useful tool for marking and tracking cells is the the ...

  12. Introducing the Addgene Antibodies 101 eBook!

    Type
    Blog Post
    ...may find “Chapter 1: What is an Antibody” quite useful. If you’re looking to design your first flow cytometry...find the latest addition to our eBook collection useful. And, as always, don’t hesitate to reach out to...

  13. Suggest A Plasmid You'd Like to Find at Addgene

    Type
    Blog Post
    ...reach everyone. Therefore, to make our library more useful to our community, we’re asking you for plasmid ...expand the repository in ways that will be most useful to you, the members of our community.  ...

  14. The Codon Guide to Synthetic Biology

    Type
    Blog Post
    ...regularly as people send me suggestions. The most useful parts of the Guide are probably the papers and ...find. I hope that many people will find this guide useful as they start their synthetic biology journey. ...

  15. Top Requested Plasmid of 2017 - pMD2.G

    Type
    Blog Post
    ...constantly evolving, but sometimes a technology is so useful it forever enhances biological research and discovery...Skimming the list, one gets a sense of the broad usefulness of pMD2.G and VSV-G. This plasmid has been used...

  16. Editor's Choice, July 2016

    Type
    Blog Post
    ...other than fluorescent proteins can be incredibly useful in FRET experiments. Professor Fessenden's example...on any of our posts if you have any questions or useful insights. If you're interested in blogging for ...

  17. Fluorescent Proteins 101: History of Fluorescent Proteins

    Type
    Blog Post
    ...Luminescent molecules are very useful tools because we can easily detect and measure the light they emit...on GFP. This great honor serves to highlight how useful fluorescent proteins like GFP really are. In this...

  18. Developing Transferable Skills During Science Training

    Type
    Blog Post
    ...leader,” develop a technical “niche,” develop a useful new assay, master a new technique and be able to... are passionate about it.   Maintain a content-useful website, write a blog Pursue speaking invitations...

  19. Top Requested AAV of 2017: pmSyn1-EBFP-CRE

    Type
    Blog Post
    ...year running, and you can learn more about this useful, DREADD-containing AAV here. But the top requested... specifically in neurons. AAV Delivered Cre - A useful tool for neuroscientists AAVs are widely used by...
Showing: 81 - 100 of 399 results