We narrowed to 302 results for: SPI

1. The Crispr Babies Saga Shows the Need for Action, Not More Delays

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   ..., and they are not remembered kindly. However, despite many headlines questioning if He’s experiments ...Harley and Lexi, and a clutter ofoutdoors-only spiders, all of whom are named Charlotte, all of whom are...

2. With Our New Viral Service, We're Taking CRISPR Further!

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   ...researcher at the Wyss Institute for Biologically Inspired Engineering and member of the Addgene Advisory...Church lab at the Wyss Institute for Biologically Inspired Engineering and the Zhang and Doench/Root labs...

3. Cultivating Community Science at BosLab

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   ...initiated a soil microbiome project. This project was inspired by previous city-wide genomic studies and our ...DIY bio If you’re not in the Boston area but are inspired to get involved, check out the local groups page...

4. Four Factors that Differentiate the Stem Cell Field

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   ...Hideyuki Okano presented ground-breaking work on spinal cord injuries; Jessica Young shared research on..., a half-brother who is a quadriplegic due to a spinal cord injury, and numerous family friends who had...

5. Viral Vectors 101: The AAV Retrograde Serotype

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   ...transduction in catecholamine pathways from the spinal cord (Wang, 2018, Metcalfe, 2022) and substantia...that are physically blocked, such as those in the spinal cord. Now that you understand this new tool, go...

6. It’s Bioelectric! An Exciting Interdisciplinary Field of Research

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   ... In frogs, ectopic expression of ion channels, despite having an otherwise normal genomic background, ...Subsequent cutting results in a two-headed worm, despite the fact that it’s genetically wild type  (Oveido...

7. Reproducibility for Everyone: Lessons from an Open Science Collaboration

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   ...science or even to discuss potential solutions. Despite all the talk, not much was being done to address...meeting by the collaborators who could participate. Despite the group not having a public website, all the ...

8. Announcing the Winners of the 2021 Michael Davidson and Roger Tsien Commemorative Conference Awards

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   ...creates a painful sensation. The dorsal horn of the spinal cord is the first site of sensory input from our... the dual virus expression in CR neurons of the spinal cord. Created with BioRender.com. Image from Haoyi...

9. Uncertainty about Labor Law Brings More Uncertainty to Postdoc Wages

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   ...institutions in Boston - Massachusetts General Hospital, Mass Eye and Ear Infirmary and Beth Israel - ...the postdoctoral population which keeps growing despite a lack of available academic positions.   Many...

10. Hot Plasmids February 2024

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    ...for your next experiments, they will also likely inspire further-improved versions. Thanks to Christophe...Leterrier, whose FocalPlanes piece on mStayGold inspired and informed this section.  Find mStayGold plasmids...

11. Viral Vectors 101: Virus Safety

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    ..., some adenovirus strains are known to cause respiratory diseases in humans, earning this virus a BSL-... can be used for extra security. Finally, when spinning down lentivirus, you’ll need to use centrifuge...

12. Gaining Leadership Skills Volunteering at a Professional Organization

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    ...postdoctoral issues first at Brigham and Women’s Hospital/Harvard Medical School and then Boston University...communication and learning to work with people despite the distance improved my verbal and written communication...

13. Experimenting in an Open Source lab: from CRISPR to Cats

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    ...the picture had a broken controller and would not spin. It was going to cost far more than it was worth...microcontroller and a motor controller and made it spin again!  We’ve also learned to be resourceful and...

14. Story of a SynBio Startup: RevBio's Epiphany (or Lack Thereof)

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    ...doing this with very little money. So when did inspiration strike us so hard that we upended our lives and...in fact, our color changing flower project was inspired by a Facebook comment.  In our day job, we worked...

15. Viral Vectors 101: Producing Your rAAV

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    ...) using a high-speed ultracentrifuge. During the spin, the virions will form a band due to their high ... https://doi.org/10.1089/hum.2010.107 Zen, Z., Espinoza, Y., Bleu, T., Sommer, J. M., & Wright, J. F. ...

16. Addgene's Cancer Collection Pages Connect the Research Community

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    ...die from cancer-related complications each year. Despite these grim statistics, new strategies for attacking...cancers, especially those of the pancreas and colon. Despite the prevalence of Ras mutations in a variety of...

17. When is a Monomer not a Monomer? The Top Three Ways Your Favorite Fluorescent Protein Oligomerizes in Cells

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    ...Snapp. Stop using EGFP/GFP for fusion proteins! Despite multiple studies in high profile journal articles...localized to the endoplasmic reticulum, the normally spiderweb-like pattern of tubules can be grossly distorted...

18. Interview: Nicola Patron on Plant Synthetic Biology, MoClo, and More

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    ... Patron: I started my Twitter account recently inspired by an academic in literature who gave a talk to...female scientists whose tweets I find really inspirational. They share experiences, articles about women...

19. INTEGRATE: Bacterial Genome Engineering Using CRISPR-Transposons

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    ...human therapeutics (e.g. using Sleeping Beauty). Despite their diverse utility, DNA transposons have been...can also be combined in single-plasmid INTEGRATE (pSPIN), which offer simple transformations for smaller...

20. AAVs in Retinal Gene Therapy

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    ...follow in the footsteps of Luxturna™. However, despite promising results in preclinical studies some potential...diseases such as hemophilia, muscular dystrophy, spinal muscular atrophy, neurodegenerative diseases and...