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We narrowed to 456 results for: SUP

Showing: 81 - 100 of 456 results

  1. Quick Guide to All Things Lentivirus

    Type
    Blog Post
    ...particles into the culture supernatant and you can generally harvest the supernatant containing viruses 24h...24h after transfection. This supernatant can be stored at 4 °C or can be directly added to your target ... process. You have to centrifuge the culture supernatant in order to precipitate the viral particles. ...Some protocols suggest adding sucrose to the supernatant to create a gradient of density that favours ...

  2. COVID-19 Resources

    Type
    Collection
    ...transmembrane glycoprotein of the immunoglobulin superfamily, binds to the SARS-CoV-2 S protein and is involved...

  3. 2022 and 2023: Reflections and Looking Forward

    Type
    Blog Post
    ...available in both trial- and full-size, with assay-supported recommended uses and education content. This work...our commitment to top notch quality service and support.  Alongside our material offerings, in 2022 we...expanding our suite of materials and services to support more research and innovation around the world. ...will remain the same is Addgene’s commitment to supporting the research community through sharing science...

  4. Making Career Development an Integral Part of the Science Training System

    Type
    Blog Post
    ...programs are still doing an inadequate job of supporting science trainee transition into diverse, successful...amplified by systemic biases which, in the absence of support and structure, prevent science from attracting ... of Massachusetts Medical School. It has been supported by a Steering Committee and working groups with...Institute of General Medical Sciences (NIGMS) will support the development and implementation of collections...

  5. Live and Let Dye: Self-Labeling Protein Tags

    Type
    Blog Post
    ... and use super-resolution or single-molecule techniques.   Figure 2: Two-color super-resolution...SNAP-tag and HaloTag ligands are available from suppliers like New England Biolabs and Promega (though if.... If you want to use far-red rhodamine dyes for super-resolution imaging, HaloTag gives better performance...Toomre, D. (2019). Labeling Strategies Matter for Super-Resolution Microscopy: A Comparison between HaloTags...

  6. Top 10 Open Science Developments of 2013

    Type
    Blog Post
    ...over $100 million in annual R&D expenditures to support increased public access to the results of federally-funded...on the global science enterprise lending their support to open scientific research data around the world...industry concerns.” 5)      In the aftermath of the Supreme Court’s decision to ban gene patents in a case ...online survey of scientists showing overwhelming support for open access to peer-reviewed journal publications...

  7. Management for Scientists: What Makes a Good Manager Anyway?

    Type
    Blog Post
    ...Behind-the-scenes support from their bosses and employers. Availability of superiors for consultation,...self-directed, but need precise leadership and support from their superiors (beware of not micromanaging the scientist...

  8. CRISPR Guide

    Type
    Collection
    ...compatible with truncated gRNAs to increase specificity SuperFi-Cas9 — increases fidelity with reduced nuclease...consecutive binding and degradation of negatively supercoiled invader DNA by cascade and CAS3. Molecular Cell...

  9. Arabidopsis in Education: How the Arabidopsis Biological Resource Center Brings Plant Science to Life

    Type
    Blog Post
    ...from the American Society of Plant Biologists and support from the National Science Foundation, ABRC’s outreach...provide in-depth, ready-to-teach lab protocols and supporting materials such as instructional videos and datasheets...classroom is that staff from the ABRC are here to support you! From questions about growing conditions, to...e-mail and phone to answer questions and provide support. Growing capacity - ABRC outreach today Over the...

  10. Addgene Receives NIH BRAIN Initiative Grant to Create Open-Access Recombinant Antibody Resource

    Type
    Blog Post
    ...Unfortunately, the vast majority of antibodies are supplied with no sequence information, leading to problems... specific activity for each reagent batch. To supplement this, Addgene will be creating a crowdsourced... new services. “We appreciate NIH’s vision and support for this project. This grant will allow Addgene... part of this initiative! The project will be supported by a NIH BRAIN Initiative grant (NS119916). The...

  11. Why Add Sucrose? Improved Yields for Adeno-associated Virus Preparation

    Type
    Blog Post
    ...Rather than being released into the cell culture supernatant, the viral particles associate with the cell ...be examined. Increased yields with sucrose supplementation is serotype specific In our study we observed...preparations for, we wanted to be certain that this supplementation strategy would have no effect on the purity... we will be spending less time and resources resupplying our current inventory and more time providing...

  12. CRISPR Plasmids - Tagging

    Type
    Collection
    ...your experiments can be found below and in the supplemental materials from the Doyon Lab’s publication: ...

  13. Behind the scenes: Addgene’s new search engine and more

    Type
    Blog Post
    ...strategies for finding what they need, and we wanted to support all of them — searching, browsing, drilling down...experts, our software team tailored Elasticsearch to support the different usage patterns the larger team identified... selecting a suggestion — how should these be supported and combined?  Luckily, we had the advantage of... information in our search indices in order to support different kinds of matches: exact words, partial...

  14. Form Your Own Peer Mentoring Group: A How-To Guide for Scientists

    Type
    Blog Post
    .... But how do you find these elusive teachers, supporters and advisors? I tried to start a more formal ...for their development plans. They all found the support of the group to be intrinsic to their success. ... continue to meet regularly, providing ongoing support, advice and accountability for development goals...mentoring relationship.  Celebrate your successes and support each other as you transition to the next stage ...

  15. The Future of Research Symposium Boston 2015

    Type
    Blog Post
    ... Training (BEST) awards, which are designed to support new approaches that address the need to train our...and from what fields – are needed to adequately supply these demands? Are there other solutions, such ...University of Maryland College Park, is a strong supporter of increasing the number of postdoctoral scientists... collection and analysis. He is also a strong supporter of science Master’s programs. This will be the...

  16. 5 Steps to Writing Grant Proposals for Grad Students

    Type
    Blog Post
    ...figure must be clear, concise, meaningful, and supportive of the mission of the application and the funding...gives the writer a chance to delegate funds to supplies, personnel, equipment, travel, etc. As a first... value of costs. It is important to be aware of supply costs per year, how many graduate students and ..., etc. It may be helpful to ask your advisor to supply a budget form used in a previously funded grant...

  17. Rabies and Neuronal Tracing

    Type
    Blog Post
    ...population. While the EnvA genetic sequences are supplied during production of EnvA-pseudotyped RABV, these...will not necessarily express EnvA unless it is supplied in trans. Monosynaptic RABV spread can be achieved...transsynaptic spread can be controlled by selectively supplying the G protein in trans only to starter cells (...replication will then occur and the G protein (supplied in trans) will be incorporated into the viral ...

  19. Plasmids 101: Secondary Nanobody Toolbox

    Type
    Blog Post
    ...which can reduce the potential image resolution of super-resolution fluorescence microscopy. Their size also...about 2 nm, which can lead to higher resolution for super-resolution microscopy. Nanobodies can be labeled... (2015). Nanobodies: site-specific labeling for super-resolution imaging, rapid epitope-mapping and native...DSHB). 1 ml of antibody-containing hybridoma supernatant is only $40, significantly cheaper than commercially...

  20. Negotiating Work and Life: How to Find the Joy

    Type
    Blog Post
    ...priorities, but many find it helpful to obtain support and ideas from those who seem to be managing. Being... When the balance tips, we need to look to for support and ideas to manage our stress. If you are saying...club, meet the neighbors and create a network of support to help with elder care, carpooling, cooking on...’ school, and with professional organizations supporting women in science and career development. I try...
Showing: 81 - 100 of 456 results