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We narrowed to 1,075 results for: TOR

Showing: 81 - 100 of 1075 results

  1. Viral Vectors 101: Inverted Terminal Repeats

    Type
    Blog Post
    ...blog Viral Vectors 101: Viral Vector Elements Viral Vectors 101: Pseudotyping Viral Vectors 101: Systemic...and are required for replication, packaging, and vector persistence. They work in conjunction with the ...retained with the gene in a recombinant AAV (rAAV) vector (Figure 1). Everything else outside the ITRs gets...gets left behind.    Figure 1: Recombinant AAV vector with ITRs that flank the gene cassette, which contains... bp version is used in most plasmids. In an AAV vector plasmid, they flank the gene of interest. A single...of the most important elements of AAV and rAAV vectors, modifications can affect gene transfer efficiency...an ITR with a sequence containing transcription factor binding sites can increase transgene expression...

  3. Viral Vectors 101: AAV Variables That Matter

    Type
    Blog Post
    ...serotypes utilize a number of different receptors, co-receptors, and other mechanisms to gain entry into...other factors can also affect your AAV experiments. Luckily, the other posts in our Viral Vectors 101 series...on the Addgene blog Viral Vectors 101: An Introduction to AAV Viral Vectors 101: The AAV Retrograde Serotype...Using AAV vectors in complex biological systems can be tricky at best, and downright infuriating at worst...know what expression pattern you need, and the factors in your system that can affect expression, before...before starting the selection process. Factors to think about are (1) your experimental conditions and measurements...testing, and optimizing three key variables of an AAV vector: serotype, promoter, and titer.     Figure...

  4. Viral Vectors 101: The AAV Retrograde Serotype

    Type
    Blog Post
    ...-dependent vectors, this can cause challenges for the experimental design as these vectors can exhibit...your system. Chemogenetic vectors  Expression of retrograde serotype vectors is present at both the injection...expression, and has retrograde functionality to monitor and map neurons (Tervo et al., 2016). With AAVrg...neuronal tracing reagents, AAV particles require receptor-mediated entry into the cell. This means that ...serotype to conduct experiments with chemogenetic vectors, this property must be taken into account since...projection neurons retrogradely transduced by the vector. One method for minimizing the impact of this phenomenon...phenomenon is to use a retrograde recombinase-expressing vector (e.g. Cre or FLP) to transduce the projection pathway...

  5. Viral Vectors 101: Viruses as Biological Tools

    Type
    Blog Post
    ...Browse Our Viral Vector Pages Learn About Viral Vector Biosafety Deposit Your Viral Vectors ... Viruses are essentially DNA (or RNA) delivery vectors, so what makes them better than the alternatives...measures in a separate, upcoming post. If a viral vector seems like the right approach for your experiment...experiment?           Click Here to Find Viral Vector Resources at Addgene! References and Resources...S, Varmus H. Retroviruses. Cold Spring Harbor Laboratory Press; Cold Spring Harbor, NY, USA: 1997. PubMed... J, et al. “Altering the tropism of lentiviral vectors through pseudotyping.” Curr. Gene. Ther. 5 (2005...

  6. New and Upcoming Viral Vectors - December 2019

    Type
    Blog Post
    ...ve added many new tools to our inventory of ready-to-use viral vectors. Here are some of the AAV we have...pAAV.hSynapsin.SF-iGluSnFR.A184S Viral vectors coming soon! These vectors should be packaged and available ... of our viral vectors blog posts Find tips for preparing AAVs Download our viral vectors 101 eBook Resources...few months. You can also browse our entire AAV inventory. Our new AAVs include: Optogenetics AAV Control...in new serotypes. See our Serotype Testing AAV inventory for the entire collection! Plasmid Serotype...norepinephrine Neuroscience research relies on accurate monitoring of neurotransmitters and we are excited to offer...

  7. History of CRISPR Cas - A tale of survival and evolution

    Type
    Blog Post
    ...Initial experiments exposed S. thermophilus to predatory phages to test if the exogenous DNA would be incorporated...system that utilized various cas genes not only to store a record of invading phages but to also destroy ...race" between phages and prokaryotes; the ultimate victor will be the species who has the strongest will ...

  8. Viral Vectors 101: AAV Serotypes and Tissue Tropism

    Type
    Blog Post
    ... growth factor receptor 1 (FGFR1)3 αVβ5 and α5β1 integrins3 Hepatocyte growth factor receptor (HGFR)3 ...3 AAV receptor (AAVR)2 Epidermal growth factor receptor (EGFR)3 AAV8 Laminin receptor (LR)3 ... Table 1: AAV capsids and receptors. Capsid Primary receptor Coreceptor AAV1 α2,3-/α2,6 N-linked...Viral Vectors 101: An Introduction to AAV Viral Vectors 101: AAV Variables that Matter Viral Vectors 101...serotypes have a primary cellular receptor and sometimes one or multiple coreceptors, which may be present on ...acids1 AAV receptor (AAVR)2   AAV2 Heparan sulfate proteoglycan (HSPG)3 AAV receptor (AAVR; not ...3 Laminin receptor (LR)3 CD93 AAV5 2,3-N-linked sialic acid4 AAV receptor (AAVR)2 Platelet-derived...

  9. Viral Vectors 101: Gamma-Retroviral Packaging Systems

    Type
    Blog Post
    ...Addgene blog Viral Vector Elements The Retroviral Life Cycle Popular Retroviral Vectors and Their Use in...  Frequently asked questions about γ-retroviral vectors How do engineered γ-retroviruses differ from lentiviruses...Woodchuck hepatitis virus post‐transcriptional regulatory element; sequence that stimulates the expression...was originally published in 2018, in the Viral Vectors 101 eBook, 1st edition.  References and resources...

  10. Twenty Years of Sharing: Addgene's Viral Vector Service

    Type
    Blog Post
    ...been finding and sharing stories unique to our repository and resources. As editor of Addgene’s eBooks — ...amount of vectors available and number of requests tell only part of the story. Viral vectors are, after...GCaMP-encoding viral vectors available in the repository. GCaMP, a calcium indicator (biosensor, by Addgene...information from requestors and depositors, and identifying trends in our own repository data, giving us...released Viral Vectors 101 2nd edition — I was incredibly excited when we picked our viral vector service as... as one of those stories. Viral vectors are viruses engineered to be safely used to deliver a genetic ...titer. Addgene’s Viral Vector Service Besides being complex to use, viral vectors are also difficult both...

  11. Viral Vectors 101: Parts of the AAV Packaging Plasmid

    Type
    Blog Post
    ...proteins from the AAV2 serotype are used to package vector genomes flanked by AAV2 ITRs into a capsid other...Strohl WR (2017) Adeno-Associated Virus (AAV) as a Vector for Gene Therapy. BioDrugs 31:317–334 . https:/...for Targeting and Improving the Efficacy of AAV Vectors. Molecular Therapy - Methods & Clinical Development...Adeno-Associated Virus Genome Interactions Important for Vector Production and Transduction. Human Gene Therapy...

  12. Safe Port in a Storm...How Addgene is Weathering the Pandemic

    Type
    Blog Post
    ...These are stormy times. It feels like we’re being buffeted by wind and waves and sometimes wonder if ...would be fully open and available to industry requestors as well. This is a time when we need as much ...materials via Addgene. Perhaps if each of many grantors supporting research could provide Addgene with...Initiative was able to step in to be our “port in the storm.”  "Addgene is a unique resource for scientists ...research is accelerating essential research in laboratories all around the world." We thank the Chan Zuckerberg...whatever lies ahead this year. We can see signs of the storm passing. We’ve recently piloted Addgene’s new cloning...cloning grade DNA service, and viral vector distribution is recovering from the pandemic-related slowdown...

  13. Viral Vectors 101: Parts of the AAV Transfer Plasmid

    Type
    Blog Post
    ...plasmid. Parts shown in grey are optional cis-regulatory elements.   Key elements of an AAV plasmid...outside of the ITRs does not get packaged in the AAV vector. The cloning capacity between the ITRs is ~4.4 ...plasmid harbors non-coding sequences. Other cis-regulatory elements Next let’s talk about some of the elements...Woodchuck Hepatitis Virus (WHP) Posttranscriptional Regulatory Element (WPRE) forms a tertiary structure when... the Addgene blog Learn more about basic viral vector elements Read about four considerations for the...

  14. Enabling high school research at the Journal of Emerging Investigators

    Type
    Blog Post
    ...we introduce you to the Journal of Emerging Investigators, an open-access journal that enables high school... the interview with the Journal of Emerging Investigators Podcast breakdown Intro Chapter 1 - Introduction...Olivia Ho-Shing, and the Journal of Emerging Investigators. Chapter 2 - Getting high school students and... building curricula and developing one-on-one mentorship programs. Chapter 6 and Outro - Interview with...

  15. Q&A With Julie Cicalese, the Newest Member of the Addgene Board of Directors

    Type
    Blog Post
    ...welcome Julie Cicalese to the Addgene Board of Directors. Cicalese served as the Chief Human Resource Officer...School. The addition of Cicalese to the Board of Directors brings human resources experience and insight ...included the Belmont Human Rights Commission and mediator for the Massachusetts small claims court summary... Meet the other members of Addgene's Board of Directors Find careers at Addgene ...

  16. Plasmids 101: A Brief History of Plasmids and an Improved eBook!

    Type
    Blog Post
    ...particles, particularly Resistance or R-factors. Like F-factors, R-factors could be transferred between bacteria...noted that, unlike F-factors, the evidence did not support the idea that R-factors could integrate into...cloning vectors such as pBR322, pACYC, and pUC were developed to provide higher copy number vectors that ...Learn more about the history of plasmids and cloning: CSHL Meeting: Plasmids: History & Biology The Joshua...were working to understand genetic cytoplasmic factors that could be transferred between cells. At the...and the labels applied to them were vague or contradictory, owing in part to the fact that very little ...little was known about the role these factors played within an organism.  So how did plasmids get their name...

  17. Story of a SynBio Startup: RevBio's Epiphany (or Lack Thereof)

    Type
    Blog Post
    ... bank account, business cards, incorporation, laboratory lease, etc. I still flip to that page in my lab...GMO food plants. When a press piece ran on the detector plants, we expected to see that same anger. Instead...business. We ended up applying to a business accelerator freely stating that we had an idea, knew how ...– $30,000 for research and another $30,000 in mentorship and business training. The money is a drop in...rough day, and we have great partners in this accelerator program who want us to succeed. We’re excited...excited to see what the next few months have in store for us. If you’re thinking about making the switch from...duty to pursue a degree in a synthetic biology laboratory and received her M.S. from CSU in 2014. Outside...

  18. mTOR Pathway

    Type
    Collection
    ...complexes, mTORC1 and mTORC2. Each complex contains a unique subunit: raptor for mTORC1, and rictor for ...for mTORC2. Background mTORC1 & mTORC2 The better-characterized mTORC1 responds to various signals, including...Ras-related GTP binding Raptor Also known as RPTOR; Regulatory associated protein of MTOR, complex 1 Rheb Ras...plasmids for the mTOR pathway. enhanced signaling of mTOR, a key metabolic regulator, correlates with ...apamycin (mTOR) is a key metabolic regulator controlling cell growth and proliferation. mTOR, a serine...inactivating mutations in negative regulators of mTORC1. The hyperactivation of mTORC1 in cancer promotes the conditions...kinase C Protor Also known as PRR5; proline rich 5 PTEN Phosphatase and tensin homolog Rictor RPTOR independent...

  19. Depositor Collections

    Type
    Collection
    ... Sigma (σ) Factor Plasmids Terminator Plasmids Viral Vectors pLEG/pREG Modular Viral Vector System - Dankort... Plasmid Collections Depositor Plasmid Collections Depositor Collections ...Addgene's repository contains 165,242 plasmids, which includes plasmid collections from depositors. To help...Collection Screening Multiplexed Overexpression of Regulatory Factors (MORF) Collection Synthetic Biology Voigt... Dankort Gene Vector Core Viral Vectors - Oka...Browse depositor collections of antibody, CRISPR, disease research, visualization, protein expression...biology, and viral vector plasmids. Plasmid...

  20. What's the Best Way to Elute and Store Your Plasmid DNA?

    Type
    Blog Post
    ...during storage over prolonged periods of time, especially at room temperature or at 4°C. Storing DNA: temperature...way to store and elute the DNA. References Murakami M (2013) Evaluation of DNA Plasmid Storage Conditions...Figure 1: So many choices! What to do? The main factors that can affect DNA stability in solution are pH...and it will impact the time you will be able to store your sample without having substantial degradation...This is because divalent cations are essential cofactors for many tools used in modern molecular biology...things when you elute your DNA: where you plan to store it and for how long. The most common solution is...
Showing: 81 - 100 of 1075 results