We narrowed to 133 results for: Tet-on

1. Management for Scientists: What Makes a Good Manager Anyway?

   Type

   Blog Post

   ...managing people: Skills for New Managers by Morey Stettner Successful Manager's Handbook: Develop Yourself...

2. Hot Plasmids and Viral Preps - September 2021

   Type

   Blog Post

   ...the expression of fluorescent proteins and oxytetracycline enzymes. Next, this system was used to recreate...

3. Plasmids 101: A Brief History of Plasmids and an Improved eBook!

   Type

   Blog Post

   ...experiment. Dr. Cohen and colleagues treated a tetracycline resistant plasmid, pSC101, and a newly developed...

4. Hot Plasmids - September 2019 - CRISPR, Auxin Inducible Degradation, MerMAID Channelrhodopsins, and Genome Looping

   Type

   Blog Post

   ...latter experiments, such as transcription factors tethered to dCas9, require long time scales with constitutively...

5. New and Upcoming Viral Vectors - June 2019

   Type

   Blog Post

   ...  AAV5 ppAAV-CKIIa-stGtACR1-FusionRed New tetracycline transactivators Plasmid Serotype Name 99120...

6. Improving Transduction Efficiency with Exosome AAVs

   Type

   Blog Post

   ...Exosome-Associated AAV by Overexpression of the Tetraspanin CD9." Molecular Therapy-Methods & Clinical Development...

7. Fluorescent Protein Travel Awards - FLiPs and Fluorescent Protein Biosensors

   Type

   Blog Post

   ...hypothesized that she could make enzymes controllable by tethering them to Dronpa, a fluorescent protein known to...

8. Pooled CRISPR Libraries Offer Genome-Wide Control for Large-Scale Functional Screens

   Type

   Blog Post

   ...sgRNA incorporating two MS2 RNA aptamers at the tetraloop and stem-loop 2; 3. The MS2-P65-HSF1 plasmid which...

9. Lighting Up Cell Signaling with Photoswitchable Kinases

   Type

   Blog Post

   ...prone to aggregation in cells compared to the tetrameric Dronpa it was derived from. What are photoswitchable...

10. Choosing the B(right)est Fluorescent Protein: Aggregation Tendency

    Type

    Blog Post

    ... In corals, the red fluorescent proteins form tetramers. In general, fluorescent proteins have a natural...

11. Plasmids 101: Cre-lox

    Type

    Blog Post

    ...orientation, allowing the four Cre proteins to form a tetramer. A double-strand DNA break occurs within the core...

12. CRISPR 101: Anti-CRISPR Proteins Switch Off CRISPR-Cas Systems

    Type

    Blog Post

    ...Acr proteins can also be used to prevent dCas9 tethering to DNA. Since these early anti-CRISPR studies,...

13. Antibodies 101: ELISA (Enzyme-linked Immunosorbent Assay)

    Type

    Blog Post

    ...reaction of HRP with its substrate, 3,3′,5,5′-Tetramethylbenzidine (TMB). The addition of hydrochloric acid...

14. Split Fluorescent Proteins for Studying Protein-Protein Interactions

    Type

    Blog Post

    ...in N-terminal fragment Constant rate of p53 tetramerization in response to DNA damage controls the p53 ...

15. Popular Retroviral Vectors and Their Uses in Scientific Research

    Type

    Blog Post

    ...You can clone your gene of interest into this tetracycline inducible, 3rd generation lentiviral vector ...

16. Quickest Way to Deposit Plasmids: The Deposit Spreadsheet

    Type

    Blog Post

    ...nourseothricin (clonNat), spectinomycin, streptomycin, tetracycline, combinations of the above, or other. High...

17. Starter guide to induced pluripotent stem cells (iPSCs) part 1:  A renaissance in regenerative medicine

    Type

    Blog Post

    ...perspective of stem cell research. Best Pract Res Clin Obstet Gynaecol, 2004. 18(6): p. 827-42. PubMed PMID: ...

18. When is a Monomer not a Monomer? The Top Three Ways Your Favorite Fluorescent Protein Oligomerizes in Cells

    Type

    Blog Post

    ...dimerization (i.e. EGFP) [1, 2] or even form obligate tetramers (i.e. DsRed) [3]. This is a problem for fusion...

19. Light Sheet Fluorescence Microscopy

    Type

    Blog Post

    ...spinal cords by combining Ultramicroscopy with tetrahydrofuran-based tissue clearing (13,14). A few years ...

20. New Acoustic Reporter Genes: Ultrasound Imaging of Gene Expression

    Type

    Blog Post

    ...transfection in rtTA+ cell lines (such as HEK293-TETOn cells), or they can also be integrated into the ...