...targeting vector assembly, and the remaining 5 plasmids include Cre and FLP expression vectors, a general...gene-specific CRISPR and donor vectors as well as pX330S-2-PITCh, expressing a generic gRNA targeting the...Plasmids: pX330S-2-PITCh - expresses Cas9 & PITCh-gRNA pX330A-FBL/PITCh - expresses Cas9, PITCh-gRNA, & FBL-specific... cloning plasmid, and a prebuilt Unc-32::GFP targeting vector. Jorgensen Lab SapTrap CRISPR/Cas Toolkit...are available. An improved FLP recombinase expression vector allows for efficient marker cassette FLP-out...cDNAs can be cloned directly into this vector and targeted to the AAVS1 genomic safe harbor locus using untagged... in combination with gRNA_AAVS1-T2 (Addgene #41818) or using an all in one vector from the Doyon lab, ...

...Commonly used cloning vectors include Gateway entry vectors and TOPO cloning vectors. If you are looking...information. Expression Plasmids - Used for gene expression (for the purposes of gene study). Expression vectors...amount of protein or RNA produced. Expression vectors can drive expression in various cell types (mammalian... the level of insert expression (i.e., a strong promoter directs high expression, whereas weaker promoters...for the transcription process to stop. An expression vector can also include an enhancer sequence which...reducing the expression of an endogenous gene. This is frequently accomplished through expression of an shRNA...combination of a DNA fragment with a plasmid or vector DNA backbone generates a recombinant DNA molecule...

...format into lentiviral transfer vectors (Figure 8B). Libraries come in 1-vector systems, in which Cas9 is included...tools require constitutive expression of the dCas9 fusion and/or expression of multiple fusions. The effects...sequences . Once you’ve selected your target, you’ll express your gRNA and Cas9 protein, which will form a ribonucleoprotein...the DSB site. Therefore, a population of cells expressing Cas9 and a gRNA will result in a diverse array...single plasmid ensures that all gRNAs will be expressed in the same cell. This is called multiplexing ...targeting in the double digits. These multiplex gRNA vectors can be used to knock out, activate, or repress ...multiplexing . Browse Plasmids: Multiplex gRNA Vectors Engineering the Cas9 Endonuclease CRISPR specificity...

...format into lentiviral transfer vectors (Figure 8B). Libraries come in 1-vector systems, in which Cas9 is included...tools require constitutive expression of the dCas9 fusion and/or expression of multiple fusions. The effects...sequences . Once you’ve selected your target, you’ll express your gRNA and Cas9 protein, which will form a ribonucleoprotein...the DSB site. Therefore, a population of cells expressing Cas9 and a gRNA will result in a diverse array...single plasmid ensures that all gRNAs will be expressed in the same cell. This is called multiplexing ...targeting in the double digits. These multiplex gRNA vectors can be used to knock out, activate, or repress ...multiplexing . Browse Plasmids: Multiplex gRNA Vectors Engineering the Cas9 Endonuclease CRISPR specificity...