We narrowed to 324 results for: cloning

Learn about the latest statistics on CRISPR plasmid deposits and distributions and how this reflects the broader CRISPR community.

Use plasmids to develop synthetic nanobodies in a few weeks with a three step selection process.

Plasmid incompatibility is the inability of different plasmids to be maintained in a cell. We’ll cover why this happens and how it might affect your experiment.

Gamma-retroviral vectors and lentiviral vectors can be used in a wide variety of experiments including those involving genome engineering, RNAi, and inducible gene expression. In this post, learn about some of the most popular viral vectors available from Addgene and their uses.

Learn about pSiM24, a new binary vector drives gene expression in plants.

Hear from the authors about the generation of Genomic Deletions in Mammalian Cell Lines via CRISPR/Cas9.

Learn how to optimize CRISPR-activated homology directed repair to make targeted edits in mammalian cells.

Learn how to use in vitro screening and other tricks to expedite the CRISPR genome editing process in mice.

In this Episode of the Addgene Podcast learn all about the Synthetic Biology research going on in Tom Ellis' lab at Imperial College London.

We've partnered with SnapGene to update our plasmid maps. Learn how to use our new maps to accelerate your research.

The Addgene repository has many amazing plasmids used by labs all over the world, but what plasmid was request most in 2015?

Addgene plasmid pages now show whether a plasmid has been cited in other articles. Use the "References" link to view the articles citing your plasmid.

Fluorescent proteins can mis-fold or form aggregates outside of the cytoplasm leading to errors in localization. Avoid these issues with new mOX-FPs.

Right variable region, but wrong constant? Chimeric antibodies may be the answer!

Looking for a plasmid that can thrive in a number of different bacteria? Learn about broad host range plasmids in this post!

The pCri plasmids available through Addgene allow researchers to multiplex the protein purification process saving time and frustration.

Learn how you can use the Broad Target Accelerator Plasmid Collections from to help enable precision cancer medicine research.

Learn about the differences in virus production between insect cells and mammalian cells.

Data-driven design of Golden Gate Assembly overhangs greatly increases the number of fragments that can be used in cloning.

Find three ways to purify genomic DNA, plasmid DNA, or PCR products without a commercial kit. Also, find a method for regenerating silica columns for reuse.