We narrowed to 229 results for: cre encoding plasmid

...cellular processes by transforming cells with plasmids encoding aptamer-tagged RNA and later introducing the...Browse fluorescent protein plasmids and resources See all Jaffrey lab plasmids ...aptamer downstream of a known promoter. Addgene’s Plasmids 101: Aptamer Fluorophores describes the enrichment...Development and characterization of sindbis virus with encoded fluorescent rna aptamer spinach2 for imaging of...for quantitative imaging, Ming Hammond’s group created Spinach2 using site-directed mutagenesis of Spinach...was cloned into bacterial expression vectors to create a library. Bacteria were transformed, and then ...for in vivo studies. Dimerization of Broccoli increases its fluorescence by about 1.8-fold, and may be...

...Flp-dependent Cre AAV in AAV9 and AAVrg. See our new additions here: Cre vectors pAAV-EF1a-fDIO-Cre (121675...is now complete! pAAV-CAG-GFP (plasmid 37825) and pAAV-hSyn-EGFP (plasmid 50465) are now available as 20...expression. This is a Cre-dependent switch that expresses nuclear mCherry in the absence of Cre, and expresses...expresses nuclear EGFP in the presence of Cre. Biosensor AAV (calcium sensors and GABA sensors) Calcium sensors... technology, see our blog post Overview of AAV Encoded Calcium Sensors). We are expanding the inventory...sensor (see below), in addition to our inventory of Cre-dependent GABA sensors. See all our GABA sensor AAV...reduced. This variant has an approximately 12-fold increase in brightness after ~100 action potentials. This...

...CRISPR plasmids available at Addgene. We will still periodically focus on specific CRISPR plasmid tools...CRISPR guide Find CRISPR plasmids by function Find recently deposited plasmids ...Biotechnology Find the base editor plasmids CRISPRi in Caulobacter crescentus Figure 1: Serial dilutions... Bioengineering Find the optogenetic repressor plasmids Cytosine base editors with minimized off-target...CRISPRi system, the Laub lab repressed ctrA, a gene encoding an essential cell cycle regulator. The lab expressed...once. Read the paper in mBio Find the CRISPRi plasmids A multiplexed gRNA piggyBac transposon system... Read the paper in Scientific Reports Find the plasmids Enhanced CRISPR-based DNA demethylation ...

...Translocation Reporters (KTRs) are genetically encoded fluorescent kinase reporters. A KTR is comprised...ERK, or a backbone to use for custom KTR design, plasmids are available from Addgene! References Kudo, ...protocols, 13 1, 155-169. PMID: 29266096. Find the KTR plasmids here! Additional Resources on the Addgene Blog...Additional Resources on Addgene.org Find FRET plasmids Find fluorescent biosensors ... key advantage of KTRs is that they are easy to create and simple to multiplex. Problems with FRET kinase...

... cas1, cas2, cas4, and a new gene cas14. cas14 encodes for a small Cas protein (40-70 kDa), which is half...regions containing the putative tracrRNA into a plasmid and expressed it in an E. coli bacteria strain....sheet Resources on Addgene.org Find the Cas14 plasmids here Checkout our CRISPR guide page Browse the...diagnostic toolkit, called DETECTR, which has been created by the authors. This kit uses Cas12 and Cas13 to...

...Collections INTRSECT Plasmid Collection INTRSECT Plasmid Collection INTRSECT Implementation Plasmids References ...of populations expressing only Cre AND Flp, Cre NOT Flp, or Flp NOT Cre. B) This strategy has been expanded...AND NOT Cre Yes 137129 pAAV-Ef1a-Con/Fon-BFP Cre AND Flp Yes 137130 pAAV-Ef1a-Con/Foff 2.0-BFP Cre AND NOT...intersectional expression plasmids that combine multiplexed recombinases, including Cre, Flp, and VCre to enable viral...expressing Cre AND Flp, here expressed through a double transgenic animal strategy (PV-2a-Cre; SOM-IRES-Flp...novel INTSRECT plasmids for groups interested in producing their own INTRSECT plasmids. Online spreadsheet...genetically-encoded calcium indicators, and rabies targeting genes. Recombinases Addgene ID Plasmid Logic Sites...

...that encode site-specific recombinases, which can be used to control gene expression. See our Cre-lox ...Cre-lox Plasmids page for more information about these molecular tools. Cre AAV ID Name Promoter Fluorophore...Deisseroth 55636 pAAV-EF1a-Cre EF1a none 11, rg* Karl Deisseroth 121675 pAAV-EF1a-fDIO-Cre EF1a none 1, 5, 8, ...Service: Recombinases Cre AAV Dre AAV Flpo AAV Light-Inducible Recombinases VCre AAV Browse the following...pAAV.CMV.HI.eGFP-Cre.WPRE.SV40 CMV eGFP 1, 2, 5, 8, 9 James M. Wilson 55632 pAAV-Ef1a-mCherry-IRES-Cre EF1a mCherry... Cepko 69916 pAAV.cTNT.iCre cTnT tdTomato 9 William Pu 203842 pAAV-Ef1a-fDIO-eGFP-2A-Cre-WPRE EF1a EGFP...Spiegel 105550 pAAV.GFAP.Cre.WPRE.hGH GFAP none 5, PHPeB James M. Wilson 196410 AAV-GfaABC1D-Cre-4x6T GfaABC1D...

... Find plasmids from this publication at Addgene.   Addgene’s Fluorescent Protein Plasmids and Resources...already have created a transgenic GFP-containing organism,” says Hanson, “using a gene encoding one of the... Methods. 2009 Feb 6(2):131-3.  PubMed. Find plasmids from this publication at Addgene. Subach OM,...Resources Relevant Blog Posts Plasmids 101: Green Fluorescent Protein Which Fluorescent Protein Should I...characterized, it turns out this protein has a few more secrets - during a collaboration, members of Maureen Hanson...necessary to observe a signal. Red signal intensity increases with GFP concentration and saturates around 30...

...selectable with the antibiotic whose resistance gene is encoded in the viral vector. This antibiotic selection ...percentage of surviving cells may be the result of plasmid integration via recombination during transfection...production of virus. Alternatively, if the virus encodes a fluorescent protein, the packaging cells could... prior to their transduction has been shown to increase AAV transduction efficiency by approximately 15...efficiency depends on viral titer Both of these can be increased by concentrating a viral stock. Different titering... virus-cell contact A variety of reagents can increase transduction efficiency. These reagents typically...reagent that is commonly used and has been shown to increase transduction efficiency by 10-fold (Davis et al...

...are transfected with plasmids expressing the GPCR of interest, a Gα-Rluc8 plasmid, and the appropriate...downstream effects.  Bryan Roth’s lab created a collection of plasmids called TRUPATH to interrogate 14 of...their mechanism. These plasmids are available as a collection, facilitating screening across the transducerome...But with approximately 950 different human genes encoding GPCRs (Takeda S et al., 2002) and many downstream...TRUPATH be used for? The Roth lab used the TRUPATH plasmids in a number of different experimental approaches...Addgene.org Browse the catalog of fluorescent protein plasmids Find biosensors for your experiments ...resulting in decreased BRET2 signal. With increased concentration of drug, the BRET2 signal decreases. ...

...spatial control. Optogenetics uses genetically encoded tools, such as microbial opsins, to control cellular...reporter gene in mice using Minicircle DNA vectors encoding the FAST constructs. This proof-of-concept model...approach. These researchers used a genetically encoded photocaging technique to insert a light-removable.... presented data showing that this genetically encoded, photocaged Cas9 system could silence endogenous...Website Browse Our Collection of Optogenetics Plasmids Check out Our Genome Engineering Resources Brush...researchers saw an 11-fold increase in mRNA levels after 2 hr and a 400-fold increase after 30 hr. The system...(CIB1) proteins. The goal of both groups was to create a system that would use light to turn on and off...

...subset of the new plasmids and viral preps in the repository through our hot plasmids articles. These articles...recent plasmid deposits and we hope they'll make it easier for you to find and use the plasmids you need...highlights from recent CRISPR plasmids. To find all of the CRISPR plasmids available from Addgene, head...melanogaster. Get the plasmids at Addgene The Cas Hybrid for Multiplexed Editing and screening Applications (...need. If you'd ever like to write about a recent plasmid deposit please sign up here.  Here's what you'll...different neurotransmitters. Using genetically encoded fluorescent proteins coupled with Fibronectin intrabodies...targeting inhibitory or excitatory synapses or that are Cre-inducible, researchers can explore synaptic integration...

...you find what you need.   More Plasmid Resources from Addgene: Plasmids 101 blog series Addgene's Molecular...need. You will also have to make sure that your plasmid has been incorporated into the host organism, usually...open reading frame (ORF) of interest –  try pRS plasmid shuttle vectors. You may prefer the more traditional...YGOI in a pattern that allows the function of the encoded protein to be assayed (e.g. pPD95_86 and pPD57_...details and guidance for choosing your perfect plasmid backbone. Or just ask –  leave a message in the...Molecular Cloning Guide & Other Plasmid Protocols List of common epitope tags ... bacteria and a mammalian selection cassette to screen for positive transfected cells. The addition of...

...Addgene.org Find plasmids for in vivo imaging with fluorescent proteins Browse luciferase plasmids Find fluorescent... Genes (ARGs) are engineered gene clusters that encode proteins that form bacterial gas vesicles. These...acoustic reporters for your research? arg1 and arg2 plasmids are available from Addgene! References 1 Bourdeau...operons from two different bacteria, Bourdeau et al created two constructs optimized for expression in E. coli... and have already developed a high-throughput screening method for serial acoustic collapse to aid in ...

...with your plasmids, and adding all the plasmids at once increases the likelihood that the plasmids are given...Unpublished or pre-publication plasmids": Unpublished plasmids or plasmids that will not be published are...and entering a plasmid name,plasmid type, and plasmid description/experimental purpose (right). On the...following descriptions for your plasmid: Encodes insert(s) A plasmid with one or more genes, protein...easy for you to share plasmids. To achieve this goal, we will archive any plasmids you've deposited with...Spreadsheet". Start your plasmid deposit There are two options to begin your plasmid deposit. Figure 2: Choose... Choose to submit plasmid(s) online or by using a spreadsheet. The plasmid information can be entered ...

...studying four of those rare diseases will receive plasmids from Addgene worth a total of $5000.  Lowe Syndrome...involving the primary cilia. They’ll put a variety of plasmids to work to explore the effect of various OCRL1...there. This will help us acquire a lot of those plasmids so that we can try many different things in parallel..., will use GFP-ATP1A3, FLAG-ATP1A3, and ATP1B3 plasmids to explore the functional differences among mutations...responsible for the disease as ATP1A3. The gene encodes a sodium-potassium pump expressed specifically ...mutations. More specifically, they’ve proposed to use plasmids with modified nickase Cas9 protein in order to...This recessive genetic condition leaves patients incredibly sensitive to sunlight and prone to developing...

...dTomato Activity Cre-dependent Flp-dependent Cre and Flp-dependent Constitutive (non-cre dependent) Serotype...Ready-to-use AAV available from Addgene's viral service encoding chemogenetics tools. Viral... in specific cell types. See our Chemogenetics Plasmids and Chemogenetics Guide pages for more information...Filters ID Name Receptor and Activity Reporter/Tag Cre requirement Serotype PI 44361 pAAV-hSyn-DIO-hM3D(...Gq)-mCherry hM3D(Gq) - Activation mCherry fusion Cre-dependent 1, 2, 5, 8, 9, rg*, PHPeB Bryan Roth 44362...Gi)-mCherry hM4D(Gi) - Inhibition mCherry fusion Cre-dependent 1, 2, 5, 8, 9, rg*, PHPeB Bryan Roth 65417...kappa-opioid receptor (KOR) - Inhibition IRES mCitrine Cre-dependent 8 Bryan Roth 50454 pAAV-hSyn-DIO-HA-hM3D...

...synthetic biology page Search for a plasmid Find Addgene's most popular plasmids ...been coming to the Addgene website in search of plasmids. Now, scientists are beginning to see Addgene ...Addgene as a large data set. Addgene has over 65,000 plasmids in the repository, each verified by sequencing...preferences for the codons they use; even if two species encode the same protein, their gene sequence may have ... recently published a tool (trained on Addgene plasmid datasets) to predict the lab-of-origin of engineered...series of simulations and machine learning, they created a classifier that predicts whether a gene was natural...

...subset of the new plasmids and viral preps in the repository through our hot plasmids articles. These articles...recent plasmid deposits and we hope they'll make it easier for you to find and use the plasmids you need... CRISPR plasmids are always being added to the repository. To find all of the CRISPR plasmids available... Xiao Wang Lab has recently deposited a set of plasmids that helps identify base edited cell populations...populations without end-point sequencing assays. These plasmids use “Cas9-mediated adenosine transient reporter...available from Addgene, head over to our CRISPR Plasmids and Resources page. Here are some highlights from the...gene. The lab modified the lentiviral backbone to encode the CD90.1 selection marker, Thy1.1, in addition...

...experiment. We’ll start with ChIP-seq data from the ENCODE project, find enhancers in embryonic stem cells...sites from 125 different human cell types from the ENCODE Project. DHS sites are the most inclusive markers...Genome Edit Resources on Addgene.org Find CRISPR Plasmids for Your Research Check out Our CRISPR Guide Find...CRISPR screen to disrupt these enhancers. The third and final step is to conduct our CRISPR screen. We start... the marvel of DNA compaction, an enhancer can increase the expression of a gene that is millions of base...cells, and conclude with a targeted CRISPR screen that can disrupt these enhancers. A real-world experiment...that have NANOG binding sites 2. Design a CRISPR screen to target and disrupt the NANOG enhancers 3. CRISPR...