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Learn how a new episomal plasmid-based method can be used to efficiently reprogram blood cells to induced pluripotent stem cells

Prime editing is a search-and-replace CRISPR editing method that offers versatility and precision in genome editing. We cover common prime editing tools.

Addgene's guide to using CRISPR plasmids in your lab, from knockouts to pooled library screening.

Joining the popular sacB counterselection method is a new suite of toxin-based allelic exchange vector that can be used in many proteobacterial genomes.

Learn about some things to consider when designing your fusions - where to make the fusions, the size and shape of the protein, and linker size.

Learn about CRISPR plant base editors for plants

Learn how to plan a basic CRISPR experiment, including selecting the edit type, designing and cloning a gRNA, delivering Cas and the gRNA, and validating the edit.

Discover the tools you need to perform plant genome engineering with CRISPR.

Restriction cloning is the cloning technique most molecular biologists start off with. Learn the ins and outs of restriction cloning here.

Explore Addgene's collection of luciferase plasmids for gene expression assays and bioluminescent reporters.

Take a look at the developments in genome engineering throughout history and the parallels in science fiction.

CRISPR Cas9 gRNA plasmids from the Zhang lab

There are many factors to consider when choosing a fluorescent dye or label. This post walks you through them so you can select a suitable one for your experiment.

Learn how stem cell researchers use reprogramming and transdifferentiation techniques to convert cells from one type into another.

Read our adeno-associated viral vector guide to learn about AAV vector components, production and common uses.

This post discusses takeaways from, and tools presented at, the Deaminet 2026 conference, focusing on base editors, prime editors, and translational work.

Designing a CRISPR experiment in bacteira? Check out these tools for genome engineering, CRISPRa, CRISPRi, base editing, and microbiome engineering.

Check out all of the plasmids featured in our hot plasmids articles from 2016.

Read on for a sampling of some of the exciting new plasmid technologies that have come through our doors in 2015.