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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 101 results for: donor

Showing: 81 - 100 of 101 results

  1. Hot Plasmids Spring 2024

    Type
    Blog Post
    ...; v2, vector 2; BD, binding domain; SDS, splice donor site; SAS, splice acceptor site; pA, polyadenylation...

  6. Plan Your Experiment

    Type
    Guide
    ... or small tag insertions, by copying from a DNA donor template Cas9 or Cas9 fusions with proteins that...HDR efficiency . Remember that HDR also requires donor DNA to serve as template for the repair. Once you...you settle on your gRNA, you can design a donor DNA to have the appropriate left and right homology arms...

  10. Luciferase Plasmid Collection

    Type
    Collection
    ...NanoBRET : Constructs using NanoLuc® as a BRET energy donor and HaloTag labeled with a fluorophore as the acceptor...

  12. Adeno-associated virus (AAV) Guide

    Type
    Guide
    ...mechanisms. One of these methods uses splice site donor and acceptor sequences to reconstitute the two pieces...contains the 5' portion of the gene and a splice donor sequence at its 3' end, while the second vector ...

  13. CRISPR Pooled gRNA Libraries

    Type
    Collection
    ...HR700_TP53 Exon Mutation Libraries 229137–229140 Donor Vector Human Stiewe N/A Varies 1,910–3,034 per exon...

  14. Zhang Lab CRISPR Page

    Type
    Collection
    ...also contains a Kras G12D homology directed repair donor template. #60225 - AAV:ITR-U6-sgRNA(LacZ)-pEFS-Rluc...)-U6-sgRNA(Lkb1)-pEFS-Rluc-2A-Cre-shortPA-KrasG12D_HDRdonor-ITR (AAV-KPL) This plasmid contains two expression...

  16. 27 Hot Plasmids from 2016

    Type
    Blog Post
    ...gene are supplied to the reaction from a prebuilt donor plasmid library, available from Addgene. Site-specific...available). Second, they co-transfected short-arm donor vectors containing mAID and either Neo or Hygro ...

  18. Genetic Code Expansion

    Type
    Collection
    ...Peter Schultz 51401 pAM1 NLL-MetRS E. coli azi-donorleucine (Anl) Bacterial ATG David Tirrell 62598 pKPY514...methionyl-tRNA synthetase (L274GMmMetRS) M. musculus azidonorleucine (Anl) Mammalian ATG David Tirrell 64915 pMAH-POLY...methionyl-tRNA synthetase (L274GMmMetRS) M. musculus azidonorleucine (Anl) Mammalian ATG David Tirrell 91705 pSupAR-MbPylRS...Mehl 172482 pEVOL-pylT-AznLRS AznLRS M. mazei azidonorleucine Bacterial TAG Wenshe Liu 173897 SepRS(2)/pSertRNA...EF1a-L274GMmMetRS-T2A-mCherry MetRS M. musculus azidonorleucine (Anl) Mammalian Elizabeth Kirby 223508 pCMV_NESPylRS...

  19. CRISPR Plasmids - Empty gRNA Vectors

    Type
    Collection
    ...pyogenes Blast Docampo pDonor_hU6 69312 Mammalian hU6 none S. pyogenes Ventura pDonor_mU6 69350 Mammalian mU6... 61285 Bacteria J23119 none S. pyogenes Beisel pZDonor-Nm-Cas9-gRNA-hU6 61366 Mammalian U6 none N. meningitidis... mU6 none S. pyogenes Ventura pDonor_sU6 69351 Mammalian sU6 none S. pyogenes Ventura pCRISPRyl 70007 ...
Showing: 81 - 100 of 101 results