We narrowed to 100 results for: donor

...CRISPR-mediated integration of large gene cassettes using AAV donor vectors." Cell reports 20.3 (2017): 750-756. PubMed...

...from CD34+ cells across blood drawn from multiple donors with non-integrating episomal vectors. PLoS One...

...This allows conjugation between a pir-containing donor and the recipient of interest. Because pDS132 cannot...

... Galphai), the C-terminus and N-terminus of the donor fluorescent protein can be truncated to increase...

...frequencies and relies on the efficient delivery of donor DNA to facilitate repair. Since the development ...

... Set up restriction digests for your insert (or donor plasmid) and plasmid backbone. Because you lose ...

... or small tag insertions, by copying from a DNA donor template Cas9 or Cas9 fusions with proteins that...HDR efficiency . Remember that HDR also requires donor DNA to serve as template for the repair. Once you...you settle on your gRNA, you can design a donor DNA to have the appropriate left and right homology arms...

...strand break (DSB) formation and do not require donor DNA, . These precise edits tend to be more efficient...

... genome editing without double-strand breaks or donor DNA. Nature 576:149–157 . https://doi.org/10.1038...

...Manufacturers will typically recommend specific fluorescent donors and acceptors in their product descriptions, or...

...NanoBRET : Constructs using NanoLuc® as a BRET energy donor and HaloTag labeled with a fluorophore as the acceptor...

...is to use cells that can be easily isolated from donors. Apart from fibroblasts, human keratinocytes from...

...also contains a Kras G12D homology directed repair donor template. #60225 - AAV:ITR-U6-sgRNA(LacZ)-pEFS-Rluc...)-U6-sgRNA(Lkb1)-pEFS-Rluc-2A-Cre-shortPA-KrasG12D_HDRdonor-ITR (AAV-KPL) This plasmid contains two expression...

...mechanisms. One of these methods uses splice site donor and acceptor sequences to reconstitute the two pieces...contains the 5' portion of the gene and a splice donor sequence at its 3' end, while the second vector ...

...crRNA, (3) three transposase proteins, and (4) the donor DNA. By using a multi-spacer CRISPR array, CRISPR-transposons...

...gene are supplied to the reaction from a prebuilt donor plasmid library, available from Addgene. Site-specific...available). Second, they co-transfected short-arm donor vectors containing mAID and either Neo or Hygro ...

... between a hook protein stably localized in the donor compartment (e.g. the ER) and a reporter protein...

...Peter Schultz 51401 pAM1 NLL-MetRS E. coli azi-donorleucine (Anl) Bacterial ATG David Tirrell 62598 pKPY514...methionyl-tRNA synthetase (L274GMmMetRS) M. musculus azidonorleucine (Anl) Mammalian ATG David Tirrell 64915 pMAH-POLY...methionyl-tRNA synthetase (L274GMmMetRS) M. musculus azidonorleucine (Anl) Mammalian ATG David Tirrell 91705 pSupAR-MbPylRS...Mehl 172482 pEVOL-pylT-AznLRS AznLRS M. mazei azidonorleucine Bacterial TAG Wenshe Liu 173897 SepRS(2)/pSertRNA...EF1a-L274GMmMetRS-T2A-mCherry MetRS M. musculus azidonorleucine (Anl) Mammalian Elizabeth Kirby 222871 pRSF-G1...

... be expressed in a single vector, pCRISPRainbow-DONOR1, with dCas9 supplied by a separate vector. ...

...Hemmo Meyer 85845 pDONOR-SNCAe2-WT SNCA CAG Parkinson's Jens Schwamborn 85847 pDONOR-SNCAe3-WT SNCA CAG...Schwamborn 85848 pDONOR-SNCAe3-A53T SNCA CAG Parkinson's Jens Schwamborn 86154 pDONOR-PARK6e5-I368N PINK1...Ataxia telangiectasia Yannick Doyon 214815 ATM_N_FS_v2_Donor ATM Ataxia telangiectasia Yannick Doyon 214916...