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We narrowed to 407 results for: expression vector

Showing: 81 - 100 of 407 results

  1. New Viral Vectors - Fall 2024

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    Blog Post
    ...the 34 new preps available in the Addgene viral vector repository! This includes the first Addgene prep...X1.1 serotype.  In rodents, AAV9-X1.1 enables expression in brain endothelial cells via an injection into...actually targets neurons, and has 45-fold higher expression levels compared to AAV9. You can now target these... AAV9-X1.1!  We also have eight new AAV-PHP.eB vectors from the Jonathan Ting lab at the Allen Institute...BRAIN Armamentarium Collection. These preps drive expression of either Cre recombinase or a fluorescent marker...carried out before. We have a bunch of other new AAV vectors as well — including next-generation biosensors ...Check them out! Table 1: New ready-to-use viral vectors in the Addgene repository  Name Serotype Category...

  2. Viral Vectors 101: What is a Virus (and a Viral Vector)?

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    Blog Post
    ...plasmids and expressed via cells cultured with the vectors. This allows the viral vectors to reproduce...Viral Vectors 101: Viral Applications Viral Vectors 101: Viruses as Biological Tools Viral Vectors 101:...create far safer viral vectors that can be used in BSL-1 or BSL-2 labs. Viral vectors are viruses that have...increase the viral vector’s carrying capacity. In order to allow the viral vectors to reproduce when experimentally...of Viral Vectors 101 posts about each type, so be sure to check our blog, or our Viral Vectors 101 eBook...viral particle is called a virion. Finally, viral vectors, which are used in the lab, are distinct from viruses...copies itself and “splits” into two cells. Viral vectors Viruses are quite appealing as molecular biology...

  3. Using AAV for Neuronal Tracing

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    Blog Post
    ...communication. Viral vectors and the nervous system With the advent of viral vector technology, new methods... at Addgene). AAV vectors: A platform for delivering neuronal tracers AAV vectors have been widely used...transgene expression. Fluorescent proteins are widely used for tracing and their expression is often made...Cre-dependent. If Cre-dependent vectors are used in transgenic animals that express Cre in only specific cell...of infected cells that also express Cre. Another advantage of AAV vectors is that there are well-established...Kohara et al. 2014 traced neurons with an AAV9 vector expressing a Cre-dependent fluorescent marker. Injection...genome is a single-stranded DNA molecule and AAV vectors are replication deficient (i.e. they cannot easily...

  4. New Viral Vectors - Summer 2024

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    Blog Post
    ...viral vectors repository since March.  Addgene is now offering an AAV2 CAG-driven tTA expresser from ...What's new in Addgene's ready-to-use viral vectors repository? Quite a bit! In this post, we'll share...this for doxycycline inducible (tet-off) gene expression! We've also added AAV1 and AAV5 preparations ... precision.   Table 1: New ready-to-use viral vectors in the Addgene repository  Name Serotype Category... multiple serotypes Looking for more viral vectors? Browse the full viral services collection or check... check out Addgene's Data Hub to see viral vector reports from other users. And don't forget to use the...below to keep up with the latest in Addgene's viral vector services and resources!   This blog post was written...

  5. New and Upcoming Viral Vectors - June 2019

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    Blog Post
    ...populations. We now offer a Flp-expressing AAV, as well as a Cre-dependent Flp-expressing AAV. See our recombinase...AAV1 pAAV-ihSyn1-DIO-tTA Viral vectors coming soon! These vectors listed below should be released at... of our viral vectors blog posts Find tips for preparing AAVs Download our viral vectors 101 eBook Resources...new tools to our inventory of ready-to-use viral vectors. Here are some of the AAV we have released in the...detection PSAM4 GlyR for chemogenetic inhibition Flp expression for intersectional studies And more! Control...Recombinase AAV Finally, we are excited to add Flp-expression AAV to our recombinase AAV inventory. Flp is ...AAV pEF1a-DIO-FLPo-WPRE-hGHp Other new viral vectors New optogenetics inhibitors Plasmid Serotype...

  6. Beyond the Blood Brain Barrier: PHP.B Enters the Central Nervous System Via the LY6A Receptor

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    Blog Post
    ... tool for gene expression in mammals. Resources on Addgene.org Search for AAV vectors to use with your...pathogens to the CNS, but this also stops viral vectors, such as adeno-associated virus (AAV), from reaching...injection into the bloodstream, but requires a high vector dose and is unable to reach all regions of the ...phenotypic effects such as those that affected gene expression, mRNA splicing, or protein coding regions. In...one, Ly6a, showed differential protein and RNA expression between the brains of C57BL/6J mice and BALB/...strong linkage to the PHP.B phenotype and its expression pattern in the mouse brain.  Both labs then took...Deverman lab started by seeing how changes in LY6A’s expression affected PHP.B’s transduction rates. Knock out...

  7. Adenoviral Delivery of CRISPR/Cas9 Aims to Expand Genome Editing to Primary Cells

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    Blog Post
    ...Cas9. Adenoviral CRISPR vectors (left) or CRISPR vectors and donor DNA vectors (right) are transduced ...need to use a two viral vectors system: one vector for Cas9/sgRNA and one vector for your specific DNA ...gRNA and Cas9 using adenoviral vectors As compared to other viral vectors, AdVs can provide a large transgene...than AAV vectors. This capacity is large enough to carry the Cas9 gene and a gRNA expression cassette ...adenoviral vectors Since then, other groups have successfully developed and used AdV vectors to edit genes...updated Dec 10, 2020 by Benoit Giquel.   Adenoviral vectors (AdVs) have been used for many years for in vivo...of oncogenicity or genotoxicity seen with other vectors (Lukashev et al., 2016). The protein capsid encapsulating...

  8. FLEx Technology and Optogenetics: Flipping the switch on gene expression with high spatial and temporal resolution

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    Blog Post
    ... optogenetic FLEx vectors in combination with transgenic animals or rAAVs expressing Cre under a specific...not naturally light-sensitive, so the selective expression of opsin genes in targeted cells makes it possible...How to get an opsin into the brain In order to express opsins within the brain, researchers inject genetically...relatively stable extra-chromosomally long-term expression and low ability to induce innate immune responses... promoters; however, those usually drive weak expression of downstream genes. Strong cell-specific promoters...Vandenberghe, 2019). How to achieve strong opsin expression in specific neuronal cell types then? This is... precise temporal and spatial control of gene expression in vivo (Schnütgen et al., 2003). This is achieved...

  9. Viral Production at Addgene

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    Blog Post
    ... with viral vector manufacturing facilities, such as the University of Pennsylvania Vector Core. Transfections...Pennsylvania Vector Core. In general, titering is performed by the facility that produced the viral vector lot....Transducibility Some viral vectors are tested in vitro and in vivo for gene expression and/or function. These...Empty vector particles can be identified after negative staining and appear darker than full vector particles...Electron micrograph of AAV vector preparation shows that the vast majority of the vectors consist of full particles...to determine which facility produced your viral vector lot. At Addgene, AAV particles are titered by droplet...method.  Titering by the University of Pennsylvania Vector Core is (as of April 2016) also performed by ddPCR...

  10. Tips for Titering Your Lentiviral Preps

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    Blog Post
    ...target cells that are positive for vector encoded transgene expression following transduction with serial...target cell line with your virus and assaying for expression of a gene carried on the transfer plasmid or ...primers that target the common features of viral vector backbones; once validated these universal primers...higher than for functional assays depending on the vector backbone. When using qPCR based methods for viral...plasmid which can over-inflate results. A packing vector deficient in reverse transcriptase will produce... prep. This method is particularly useful when vectors carrying fluorescent markers are used as it eliminates...addition, promoter choice can also impact transgene expression. The most accurate method of lentiviral titration...

  11. Plasmids 101: Knockout/Knock-In Plasmids

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    Blog Post
    ...: A knockout targeting vector designed to insert a resistance gene. The vector contains a neomycin resistance... Resources on Addgene.org Find Mammalian Expression Vectors Find Cre-Lox Plasmids Learn More about CRISPR... homologous sequence using a specially designed vector that contains sequence homology to the locus of... of the targeted gene have been cloned into the vector on either side of an antibiotic resistance gene...Figure 2). Figure 2: A knockout targeting vector designed to insert and later remove a resistance... the sequence to be inserted is cloned into the vector between the homologous sequences together with ...disrupted (knocked out) but the inserted GFP is expressed (knocked in). As seen in the above example, you...

  12. Plasmids 101: Broad Host Range Plasmids

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    Blog Post
    ...to create recombinant vectors that can replicate in different bacteria and express proteins within different... S. (2011). Broad-host-range plasmid vectors for gene expression in bacteria. Methods in Molecular Biology...Gill, R. T. (2010). Broad-host-range vectors for protein expression across gram negative hosts. Biotechnology...Cloning Vectors for Gram-Negative Bacteria. In R. L. Rodriguez & D. T. Denhardt (Eds.), Vectors (pp. 287... inform us on how to create better recombinant vectors. In this blog post, we’ll define plasmid host range...features to create broad host range recombinant vectors.  A plasmid’s host range is determined by the ...groups (Jain and Srivastava 2013).  Recombinant vectors that are made from broad host range plasmids are...

  13. Adenoviral Vector Production and Troubleshooting

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    Blog Post
    ...Adenoviral vectors (AdV) are attractive vectors for research applications and gene therapy: they can ...Three generations of adenoviral vectors: the first generation vectors are deficient in the E1 and E3 regions... second generation vectors are deficient in E1, E2, E3, and E4. Gutless Ad vectors (helper-dependent (...creating adenoviral vector constructs. It consists of 2 plasmids: a shuttle vector (in which the transgene...death of transduced cells and loss of transgene expression (Interestingly, the strong immunogenicity of ...Adenovirus type 5. Three generations of adenoviral vectors The adenovirus virion is a non-enveloped particle...capacity is limited to 8.2 kb, and in vivo transgene expression ceases relatively quickly due to immune response...

  14. Plasmids 101: The Promoter Region – Let's Go!

    Type
    Blog Post
    ...Description Expression Additional considerations CMV General expression mRNA Strong mammalian expression promoter...lower expression than U6. May have better expression in neuronal cells. U6 small RNA expression shRNA...some cell types. EF1a General expression mRNA Strong mammalian expression from human elongation factor ...give consistent expression regardless of cell type or physiology. SV40 General expression mRNA Mammalian...  Commonly used in expression systems for Drosophila. Polyhedrin General expression mRNA  Strong insect...Constitutive  Commonly used in expression systems for insect cells. CaMKIIa Gene expression for optogenetics mRNA...neuronal/CNS expression. Modulated by calcium and calmodulin. GAL1, 10 General expression mRNA Yeast ...

  15. Viral Vectors 101: Types of viruses

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    Blog Post
    ...Viral Vectors 101: Viral Vector Elements Viral Vectors 101: An Introduction to AAV Viral Vectors 101:...integrate into proto-oncogenes and disrupt their expression (Robinson, 1982). To add insult to injury, retroviruses...cells and doesn’t require genomic integration to express its cargo, in fact its viral life cycle does not...not include genomic integration. While the expression of adenovirus cargo is high, it’s generally not ... effective option if you need high, transient expression of a genetic cargo or have a very large cargo... Galla M, Baum C, Schambach A. Gammaretroviral vectors: biology, technology and application. Viruses. ...

  16. CRISPR 101: Mammalian Expression Systems and Delivery Methods

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    Blog Post
    ...System Application Mammalian expression vector Promoter driving Cas9 expression can be constitutive or inducible...included with each expression system as well as suitable applications. Expression System Components ... in a single lentiviral transfer vector or separate transfer vectors. May contain reporter gene (e.g. ... AAV transfer vector and used to generate AAV particles. Transient or stable expression of SaCas9 and/...or electroporation. Transient expression of CRISPR components, expression decreases as RNA is degraded ...using cationic lipids. Transient expression of CRISPR components, expression decreases as gRNA and Cas9 protein... provide an overview of some CRISPR mammalian expression systems, the typical applications for each, and...

  17. Quick Guide to Working with Drosophila Part 3: Genome Engineering in Flies

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    Blog Post
    ... plasmids for overexpression and knockdown. Addgene also has a number of insect vectors to consider. An... your gene of interest in a plasmid and get the vector incorporated into the germline. To begin this process... browse Addgene’s Collection under the insect expression system or by filtering by fly as species of gene...Find Gal4 and UAS plasmids at Addgene Choosing a vector to generate a transgenic fly There are a few important...important points to consider when choosing the vector you’d like to use to generate your new fly line:... gene to be incorporated into the genome? Some vectors incorporate into the genome randomly, while others...insertion has the advantage that you can get varying expression levels of your transgene in different fly lines...

  18. Hot Plasmids - March 2019 - Anti-CRISPR, 2in1 Cloning, Fluorescent Voltage Indicators, and Photoswitchable Proteins

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    Blog Post
    ... cloned into an entry vector. The 2in1 cloning system contains two entry vectors (pUC57-L1L4 and pUC57... of protein coexpression. By introducing a single plasmid containing both GOIs, expression levels are ...-protein interaction studies easier with a 2in1 vector cloning system Article contributed by Michelle ... Recently, the Grefen lab introduced the “2in1” vector cloning system - a Gateway compatible approach ... genes-of-interest (GOI) into two independent expression cassettes on a single plasmid (Grefen and Blatt...digest cloning to introduce the GOIs into the 2in1 vector instead of the GatewayTM cloning BP reaction, where...Agrobacterium-mediated DNA transfer, each GOI is usually expressed from a different plasmid, which may lead to unequal...

  19. Educational Planarian Tools for Teaching Developmental Biology Techniques

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    Blog Post
    ...interference (RNAi) to knockdown expression of specific gene expression. The 49 deposited plasmids enable... all have a PR-T4 vector backbone, which is a modified version of L440. This vector is versatile in that...of interest into the empty PR-T4P vector backbone. The empty vector PLL4, which is similar to PR-T4P, ...Ingestion of bacterially expressed double-stranded RNA inhibits gene expression in planarians." Proceedings...Scientists routinely use techniques to alter gene expression or to label specific cells, but there are too... in Situ Hybridization (WISH) for visualizing expression of genes. Double-stranded RNA (dsRNA)-mediated...enable students to study gene expression and functions of key regeneration-related genes in four inexpensive...

  20. Adeno-associated Viruses (AAVs) for Genome Editing

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    Blog Post
    ...selectable markers that are only expressed when the targeting vector is inserted downstream of a functional...them sequentially into the chosen acceptor vector. The vectors are then packaged into AAV virions via transient...Check out our Viral Service Find AAV Vectors Browse all Viral Vectors ...fantastic gene delivery vehicles for episomal gene expression and are particularly useful for gene delivery...well understood, researchers have been using AAV vectors for gene editing in mammalian cells since 1998....when delivered via AAV infection, gene targeting vectors with relatively short (1 kb) homology arms underwent...orders of magnitude higher than identical targeting vectors delivered through transfection of naked DNA. Other...
Showing: 81 - 100 of 407 results