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...Chemistry, 15(7), 7. https://doi.org/10.1038/s41557-023-01214-0. Preprint on bioRxiv: https://doi.org/10.1101...organizer development. PLoS Genetics, 19(5), e1010765. https://doi.org/10.1371/journal.pgen.1010765.  Berlew,...Toolbox. ACS Synthetic Biology, 11(1), 515–521. https://doi.org/10.1021/acssynbio.1c00604.  Mahlandt, ...endothelial barrier strength. eLife, 12, RP84364. https://doi.org/10.7554/eLife.84364.  Meiring, J. C. M...disassembly. Current Biology, 32(21), 4660-4674.e6. https://doi.org/10.1016/j.cub.2022.09.010.  Ridley, A..... Current Opinion in Cell Biology, 36, 103–112. https://doi.org/10.1016/j.ceb.2015.08.005.  Tei, R., Bagde...

...heterogeneous samples. bioRxiv 2024.01.21.576499; doi: https://doi.org/10.1101/2024.01.21.576499.    LEA proteins...plunge freezing. bioRxiv 2024.02.06.579238. doi: https://doi.org/10.1101/2024.02.06.579238.    Engineered...Biotechnology, 10.1038/s41587-023-02078-y. doi: https://doi.org/10.1038/s41587-023-02078-y.  Anzalone,...editing. Nature Biotechnology, 40(5), 731–740. doi: https://doi.org/10.1038/s41587-021-01133-w Pandey, S.,... Engineering, 10.1038/s41551-024-01227-1. doi:  https://doi.org/10.1038/s41551-024-01227-1   CHARM: A...epigenetic editor. Science, 384(6703), ado7082. doi: https://doi.org/10.1126/science.ado7082.  Nuñez, J. K....epigenome editing. Cell, 184(9), 2503–2519.e17. doi: https://doi.org/10.1016/j.cell.2021.03.025.    Recombinant...

.... Volume 14. 2017, Pages 19-28, ISSN 2214-7535, https://doi.org/10.1016/j.bdq.2017.11.001.Bustin SA; Benes...Volume 27, Issue 7, 1 April 2011, Pages 1019–1020, https://doi.org/10.1093/bioinformatics/btr065Freeman WM... Reprogramming Process. Sci Rep 8, 8716 (2018). https://doi.org/10.1038/s41598-018-26707-8Suzuki T; Higgins...publication quality data. Sci Rep 7, 2409 (2017). https://doi.org/10.1038/s41598-017-02217-xTaylor, SC; ...37, Issue 7, 2019, Pages 761-774, SSN 0167-7799, https://doi.org/10.1016/j.tibtech.2018.12.002.Teo IA; ... 28, Issue 19, 1 October 2000, Pages 3752–3761, https://doi.org/10.1093/nar/28.19.3752Wong M; Medrano ...mRNA quantitation. Biotechniques, 1, 30 (2018). https://doi.org/10.2144/05391RV01 Yuan JS; Reed A; Chen...

... 8 and 9 in the Mouse Brain. PLoS ONE 8:e76310. https://doi.org/10.1371/journal.pone.0076310 Burger C,...Central Nervous System. Molecular Therapy 10:302–317. https://doi.org/10.1016/j.ymthe.2004.05.024 Dudek AM, ... Entry Independent of AAV Receptor. J Virol 92. https://doi.org/10.1128/jvi.02213-17 Fischer KB, Collins...vectors. Proc Natl Acad Sci USA 116:27001–27010. https://doi.org/10.1073/pnas.1915974116 Guerin K, Rego...Viral DNA Extracts. Human Gene Therapy 31:664–678. https://doi.org/10.1089/hum.2019.277 Lavin TK, Jin L, ...Virus Concentrations. Front Synaptic Neurosci 12. https://doi.org/10.3389/fnsyn.2020.00006 O’Carroll SJ,...Relevance to Human Gene Therapy. Front Mol Neurosci 13. https://doi.org/10.3389/fnmol.2020.618020 Additional resources...

...gene therapy.” Journal of cellular physiology.  https://doi.org/10.1002/jcp.30064 2. Barman et al. 2020...with CRISPR-Cas9 technology.” Current genetics. https://doi.org/10.1007/s00294-019-01040-3 3. Khatibi ... Review.” Current pharmaceutical biotechnology. https://doi.org/10.2174/1389201021666200621161610 4. Uddin...and Implications for the Future.” Front. Oncol. https://doi.org/10.3389/fonc.2020.01387 5. Rohn et al.... Journal of Alzheimer's disease & Parkinsonism. https://doi.org/10.4172/2161-0460.1000439 6. Wu et al....Therapy in Human Genetic Diseases.” Theranostics. https://doi.org/10.7150/thno.43360 7. Yuxuan Wu et al....

...Potency of AAV Vectors. Microorganisms, 12(2), 310. https://doi.org/10.3390/microorganisms1202031038399714...Transgene Expression. Hum Gene Ther, 31(3–4), 151–162. https://doi.org/10.1089/hum.2019.274. PMID: 31914802. ... System. Hum Gene Ther Methods, 28(5), 277–289. https://doi.org/10.1089/hgtb.2016.133. PMID: 28967288....capsid protein modification. Front Med, 10, 1106085. https://doi.org/10.3389/fmed.2023.1106085. PMID: 36817775...Adeno-Associated Virus. Hum Gene Ther Methods, 30(6), 206–213. https://doi.org/10.1089/hgtb.2019.276. PMID: 31752513....increases transgene expression. Sci Rep, 7(1), 5432. https://doi.org/10.1038/s41598-017-04054-4. PMID: 28710345...

...Its Mutagenic Potential. PLoS Genet 5:e1000683 . https://doi.org/10.1371/journal.pgen.1000683 Bétermier...Inherently Error-Prone Process? PLoS Genet 10:e1004086 . https://doi.org/10.1371/journal.pgen.1004086 Ghezraoui...Nonhomologous End-Joining. Molecular Cell 55:829–842 . https://doi.org/10.1016/j.molcel.2014.08.002 Ran FA, Hsu...Genome Editing Specificity. Cell 154:1380–1389 . https://doi.org/10.1016/j.cell.2013.08.021 Waters CA, ...during nonhomologous end joining. Nat Commun 5: . https://doi.org/10.1038/ncomms5286 Yan M-Y, Li S-S, Ding...Editing in Mycobacterium tuberculosis. mBio 11: . https://doi.org/10.1128/mbio.02364-19 More CRISPR Blog...

... guide to cytometry. Trends Immunol 33:323–332. https://doi.org/10.1016/j.it.2012.02.010 Dennler P, Fischer...Populations to Defined Reagents. Antibodies 4:197–224. https://doi.org/10.3390/antib4030197  Hegazy M, Cohen-...Tissues in Situ. Curr Protoc Cell Biol 89:e115. https://doi.org/10.1002/cpcb.115  Mueller BM, WrasidloO... to Monoclonal Antibodies. Hybridoma 7:453–456. https://doi.org/10.1089/hyb.1988.7.453  Niemeyer CM, Adler...quantitative immuno-PCR. Nat Protoc 2:1918–1930. https://doi.org/10.1038/nprot.2007.267 Stoeckius M, Hafemeister...measurement in single cells. Nat Methods 14:865–868. https://doi.org/10.1038/nmeth.4380  Additional resources...

... 1502–1513. doi: https://doi.org/10.1038/s41477-024-01786-w. (bioRxiv preprint: https://doi.org/10.1101...absence of cargo. bioRxiv 2024.12.20.629623; doi: https://doi.org/10.1101/2024.12.20.629623.  A fast and...synaptic events. Neuron, 112(22), 3680–3696.e8. doi: https://doi.org/10.1016/j.neuron.2024.08.019.  ONE-GO...biosensor platform. Cell, 187(6), 1527–1546.e25. doi: https://doi.org/10.1016/j.cell.2024.01.028. New recombinant...Immunohistochemistry (Human). Addgene Report, doi: https://doi.org/10.57733/addgene.6ncijt.  ...

...vivo screens. Nature Protocols, 17(9), 1903–1925. https://doi.org/10.1038/s41596-022-00700-y Hanlon, K. ...CRISPR-induced DNA breaks. Nature Communications, 10(1). https://doi.org/10.1038/s41467-019-12449-2 Pupo, A., Fernández... therapy. Molecular Therapy, 30(12), 3515–3541. https://doi.org/10.1016/j.ymthe.2022.09.015 Ramani, B....platform. bioRxiv (Cold Spring Harbor Laboratory). https://doi.org/10.1101/2023.06.13.544831 Santinha, A....vivo AAV-Perturb-seq. Nature, 622(7982), 367–375. https://doi.org/10.1038/s41586-023-06570-y Wang, J., Gessler...Signal Transduction and Targeted Therapy, 9(1). https://doi.org/10.1038/s41392-024-01780-w Additional ...

...share your lab protocols online Addgene Website: http://www.addgene.org/ If you’re working with plasmids...started on our deposit page. Protocols.io Website: http://protocols.io/ Protocols.io is the creation of ... the resource. Nature Protocol Exchange Website: http://www.nature.com/protocolexchange/ Nature Protocol... and biomedical topics. Protocol Online Website: http://www.protocol-online.org/ Protocol Online is one... find useful for your research. LabGuru Website: http://www.labguru.com/ Labguru is a good place to go...such as Nature Protcols and NEB. Quartzy Website: https://www.quartzy.com/ Quartzy is a free web-based lab...

...protein–protein interactions in live Drosophila. eLife 7. https://doi.org/10.7554/elife.38853 Hu C-D, Chinenov Y...Fluorescence Complementation. Molecular Cell 9:789–798. https://doi.org/10.1016/s1097-2765(02)00496-3 Kerppola...interactions in living cells. Nat Protoc 1:1278–1286. https://doi.org/10.1038/nprot.2006.201 Kerppola TK (2013...Cells. Cold Spring Harb Protoc 2013:pdb.prot076497. https://doi.org/10.1101/pdb.prot076497 Kim Y, Jung JP,...Saccharomyces cerevisiae. Genome Res 29:135–145. https://doi.org/10.1101/gr.231860.117 Kodama Y, Hu C-D...year update and future perspectives. Biotech 53. https://doi.org/10.2144/000113943 Additional resources...

... DNA without DNA cleavage. Nature 551:464–471 . https://doi.org/10.1038/nature24644 Glaser A, McColl B...Editing. Molecular Therapy - Nucleic Acids 5:e334 . https://doi.org/10.1038/mtna.2016.48 Katti A, Foronda ...activity. Nucleic Acids Research 48:2841–2852 . https://doi.org/10.1093/nar/gkaa124 Martin AS, Salamango...APOBEC-Cas9 editosome complexes. Scientific Reports 9: . https://doi.org/10.1038/s41598-018-36739-9 St. Martin ...living cells. Nucleic Acids Research 46:e84–e84 . https://doi.org/10.1093/nar/gky332 Standage-Beier K, Tekel...human cells. Nucleic Acids Research 47:e120–e120 . https://doi.org/10.1093/nar/gkz713 Additional resources...

...nucleobase detection and identification using FnCas9. https://doi.org/10.1101/2020.04.07.028167 Broughton JP... detection of SARS-CoV-2. Nature Biotechnology. https://doi.org/10.1038/s41587-020-0513-4 Kellner MJ, ...CRISPR nucleases. Nature Protocols 14:2986–3012 . https://doi.org/10.1038/s41596-019-0210-2 Lucia, C., Federico...on CRISPR-Cas12. Cold Spring Harbor Laboratory. https://doi.org/10.1101/2020.02.29.971127 Rauch, J. N....Genetic Material. Cold Spring Harbor Laboratory. https://doi.org/10.1101/2020.04.20.052159 Zhang, F., Abudayyeh...detection of COVID-19 using CRISPR diagnostics. https://www.broadinstitute.org/files/publications/special...

...protein analyses using ELISA. Peptides 72:4–15 . https://doi.org/10.1016/j.peptides.2015.04.012 Burnette...protein A. Analytical Biochemistry 112:195–203 . https://doi.org/10.1016/0003-2697(81)90281-5 Pillai-Kastoori...the user, for the user. J Biol Chem 295:926–939 . https://doi.org/10.1074/jbc.ra119.010472 Renart J, Reiser...the National Academy of Sciences 76:3116–3120 . https://doi.org/10.1073/pnas.76.7.3116 Towbin H, Staehelin...the National Academy of Sciences 76:4350–4354 . https://doi.org/10.1073/pnas.76.9.4350 Yang P-C, Mahmood...and trouble shooting. North Am J Med Sci 4:429 . https://doi.org/10.4103/1947-2714.100998 Additional resources...

...multiplex immunolabeling of brain. eLife, 8, e43322. https://doi.org/10.7554/eLife.43322 Brewer, M., McDonough...Immunofluorescence on Fresh Frozen Tissue-V2 v2. protocols.io. https://doi.org/10.17504/protocols.io.bs68nhhw Im, K.,...Biobanking (Vol. 1897, pp. 299–311). Springer New York. https://doi.org/10.1007/978-1-4939-8935-5_26 Jambor, H...scientific publications. PLOS Biology, 19(3), e3001161. https://doi.org/10.1371/journal.pbio.3001161 McLaughlin...Immunohistochemistry. Materials and Methods, 9. https://doi.org/10.13070/mm.en.9.2846 Radtke, A. J., Kandov...National Academy of Sciences, 117(52), 33455–33465. https://doi.org/10.1073/pnas.2018488117 Additional Resources...

...–1547.e15. doi: https://doi.org/10.1016/j.neuron.2025.05.002. bioRxiv preprint: https://doi.org/10.1101...deactivation rates. bioRxiv, 2025.03.20.643984. https://doi.org/10.1101/2025.03.20.643984.  Build your...retrotransposon activity to new DNA sites. Nature. doi: https://doi.org/10.1038/s41586-025-08877-4.  Two powerhouse...interactome. Molecular Cell, 84(24), 4929-4944.e8. https://doi.org/10.1016/j.molcel.2024.11.007. Branon, ... TurboID. Nature Biotechnology, 36(9), 880–887. https://doi.org/10.1038/nbt.4201. Hot viral preps: Enhancer-AAVs...

...plasmid in Escherichia coli Stbl3. 3 Biotech 3:61–70. https://doi.org/10.1007/s13205-012-0070-8   Chakiath ...reduced-genomeEscherichia coli. BioTechniques 43:466–470. https://doi.org/10.2144/000112585   Ding T, Huang C, Liang...editing in Escherichia coli. Microb Cell Fact 19. https://doi.org/10.1186/s12934-020-01321-4   Kolodner ... the National Academy of Sciences 77:4847–4851. https://doi.org/10.1073/pnas.77.8.4847 Trepotec Z, Geiger...translation efficiency or half-life. RNA 25:507–518. https://doi.org/10.1261/rna.069286.118 Additional resources...

... I excluded here for better visibility. Source: https://www.bdbiosciences.com/en-us/resources/bd-spectrum-viewer...relation to the laser type. Table adapted from: https://emea.bd.com/panel-design/en/flow-cytometry-fluochrome-brightness-spillover...depending on the laser light's wavelength. Source: https://www.bdbiosciences.com/en-us/resources/bd-spectrum-viewer... at higher wavelengths of about 750 nm. Source: https://www.bdbiosciences.com/en-us/resources/bd-spectrum-viewer...cannot use them together in the same panel. Source: https://www.bdbiosciences.com/en-us/resources/bd-spectrum-viewer...fluorophores used in our panel (Table 2). Source: https://www.bdbiosciences.com/en-us/resources/bd-spectrum-viewer...

...observational study. The Lancet Microbe 1:e14–e23 . https://doi.org/10.1016/s2666-5247(20)30004-5 McCray PB...Respiratory Syndrome Coronavirus. JVI 81:813–821 . https://doi.org/10.1128/jvi.02012-06 Ou X, Liu Y, Lei ...cross-reactivity with SARS-CoV. Nat Commun 11: . https://doi.org/10.1038/s41467-020-15562-9 Subbarao K,...model for SARS? Trends in Microbiology 14:299–303 . https://doi.org/10.1016/j.tim.2006.05.007 (2020) Open ...Open for outbreaks. Nat Biotechnol 38:377–377. https://doi.org/10.1038/s41587-020-0499-y Additional resources...