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We narrowed to 302 results for: human dat

Showing: 81 - 100 of 302 results

  1. GPCRs: How Do They Work and How Do We Study Them?

    Type
    Blog Post
    ...estimated that there are approximately 950 different human genes encoding GPCRs (Takeda et al., 2002). G proteins...2016). DREADDs were engineered from members of the human muscarinic receptor family and are activated by ...open-source resource for interrogation of the druggable human GPCRome. Nat Struct Mol Biol 22:362–369 . https:...Identification of G protein-coupled receptor genes from the human genome sequence. FEBS Letters 520:97–101 . https...lab also assembled a collection of plasmids to elucidate the role of 14 of the 16 non-visual heterotrimeric...transducing receptors in the brain they are ideal candidates for use in chemogenetics. To create chemogenetics...

  2. CRISPR 101: Multiplex Expression of gRNAs

    Type
    Blog Post
    ...target sequence downstream of the 7SK, human U6, mouse U6, or human H1 promoters. If you express fewer than...lab. pX333, a modification of pX330, contains humanized wtCas9 and two U6 promoters. To use this plasmid...destination vectors. Destination vector options include humanized wt Cas9, dCas9 (transcriptional repressor), and...plasmids, multiple Cas9 variants are available: wt humanized Cas9, D10A nickase mutant (Cas9n), dCas9 (transcriptional...used to create lentiviral vectors expressing wt humanized Cas9 and up to three gRNAs. Entry vectors containing...Tetsushi, et al. “Multiplex genome engineering in human cells using all-in-one CRISPR/Cas9 vector system...Originally published Jan 28, 2016 and last updated Sep 10, 2020 by Jennifer Tsang. CRISPR makes it easy...

  3. Viral Vectors 101: Viruses as Biological Tools

    Type
    Blog Post
    ...infection into a host cell. Image courtesy of National Human Genome Research Institute. Its genome, however...across applications, while electroporation of a human, for example, isn’t a reasonable option. Since ... This feature makes them an attractive tool for human subject delivery, as immunogenicity was previously... Bukrinsky MI, et al. “Active nuclear import of human immunodeficiency virus type 1 preintegration complexes...disassembly. Both lenti and retro viruses can accommodate a cargo up to approximately 8 kb, but viral packaging...

  4. Gendered Innovations: Why Does Sex of the Cell Matter?

    Type
    Blog Post
    ...market cost billions—but when drugs fail, they cause human suffering and death. Doing research right has the...therapeutic interventions you develop represent the whole human population. To learn a little more about where differences...stage of research is minimal compared to that of human clinical trials. And, as Janine Clayton points out...incomplete (because sex was not analyzed) may harm humans or contribute to failures in drug development –... European Commission, the US National Science Foundation, and Stanford University—that harnesses the creative...

  5. Uncovering Ancestral Bias in CRISPR Research

    Type
    Blog Post
    ...ancestral bias and the human genome Wang, T., Antonacci-Fulton, L., Howe, K. et al. The Human Pangenome Project...Fultineer analyzed the gnomAD dataset, which captures a broader swath of human genetic diversity, to better...represent the diversity of genetic variation across the human population. Rather, it primarily reflects European...lead to new cancer targets and related biomarkers . Data from such screens are often uploaded to the Cancer...Cancer Dependency Map (DepMap), a cancer dependency database used to make this information accessible to the...Fultineer in the Beroukhim and Boehm labs pulled data from more than 1000 CRISPR screens in DepMap to ...these strange results by digging deeply into their data.     Fig. 1: Genomic fraction for each of...

  6. CRISPR Between the Genes: How to Experiment with Enhancers and Epigenomics

    Type
    Blog Post
    ...generated the DHS data set by starting with all of the DHS sites from 125 different human cell types from...biology. GPS for the genome We can think of the human genome as a map with three coordinates: chromosome...,000 significant binding peaks for NANOG in the human genome. But not all of those 13,000 regions are ... effect on unhealthy cells. Until recently, the human genome has been full of dark matter: enhancers, ...! Aneesh Karve is CTO at Quilt Data. Quilt is a collaborative database. Aneesh's research interests span...contributed by guest blogger, Aneesh Karve, CTO at Quilt Data. This post was originally published on the Quilt...here with permission. Quilt is a collaborative database for genomics. In this article, Quilt CTO Aneesh...

  7. To Codon Optimize or Not: That is the Question

    Type
    Blog Post
    ...For example, it may be worth codon optimizing a human gene for expression in E. coli. Genes are also optimized...some diseases. It has been reported that 5-10% of human genes contain a region where a synonymous mutation...2014) A critical analysis of codon optimization in human therapeutics. Trends in Molecular Medicine 20:604...Understanding the contribution of synonymous mutations to human disease. Nat Rev Genet 12:683–691. https://doi.org...Usage Tabulated from GenBank database (CUTG), that provides an electronic dataset for codon-usage of 257,468... Simonyan V, Kimchi-Sarfaty C (2017) A new and updated resource for codon usage tables. BMC Bioinformatics...usage tabulated from international DNA sequence databases: status for the year 2000. Nucleic Acids Research...

  8. Antibodies 101: Chimeric Antibodies

    Type
    Blog Post
    ...process, antibodies derived from non-human species like mice are “humanized” by replacing as much of the non-antigen...non-antigen binding mouse sequence as possible with human antibody sequences.  Additional considerations ...antibodies are both extensively published, knockout validated, and work in your application. But…they are the...differences are subtle, chimeric antibodies should be revalidated before use. In addition, chimeric antibodies ...resources on the Addgene blog Antibodies 101: Validation Antibodies 101: Polyclonal Antibodies Antibodies...

  9. Important Considerations When Using AAVs

    Type
    Blog Post
    ... The use of AAVs in humans AAVs are already being used to treat disease in humans and additional improvements...are suspected cases of insertional mutagenesis in humans (1) and AAVs are non-enveloped viruses, so they...for anterograde and retrograde axonal transport." Human gene therapy 25.8 (2014): 705-720. PubMed PMID: ...and the role of rep proteins in vector purity." Human gene therapy 18.2 (2007): 171-182. PubMed PMID: ...therapy: Platform for RNAi, Gene Editing and Beyond." Human Gene Therapy ja (2017). PubMed PMID: 28073291. Additional...predict the perfect titer, but one of the main recommendations when using AAVs is to avoid freezing and thawing...

  10. Adeno Associated Virus (AAV) for Cell and Gene Therapy

    Type
    Blog Post
    ...great promise for improving or potentially curing human diseases that were previously thought to be incurable...quickly identify underlying genetic causes of some human conditions, opening up new avenues for therapeutics...subretinal administration of rAAV2 carrying the human gene RPE65, patients with clinical diagnosis of ...and 5 are thought to be as high as 70% among the human population. These pre-existing antibodies can interfere...lack of immune cell development and function. To date, gene therapy has been used to treat 10 infants ...

  11. Predicting Adverse Reactions to Monoclonal Antibody Drugs

    Type
    Blog Post
    ...they are designed to mimic the human lymphatic system or even the human skin and subcutaneous models (...blood mononuclear cells (PBMC) in 3D models of the human lymphatic system. PBMCs consist of various immune... These algorithms are trained on vast amounts of data to calculate the relative immunogenicity score (...of in vitro prediction of immunogenicity is to validate the findings from the in silico assessments, as...Korte, E. Schenck, & G. F. Weinbauer (Eds.), The Nonhuman Primate in Nonclinical Drug Development and Safety...

  12. Visualizing Genomic Loci with CRISPR-Sirius

    Type
    Blog Post
    ...labeling system to visualize chromosomal loci in human cells (Ma et al., 2015). To do so, they fused a ..., 26 of the 46 loci gave a detectable signal in human U2OS cells. Based upon this, they chose an intergenic...Multicolor CRISPR labeling of chromosomal loci in human cells.” Proceedings of the National Academy of Sciences...CRISPR-Sirius, the brightest CRISPR-based tool to date for visualizing genomic loci in living cells. Previous...in CRISPRainbow, they knew they could be good candidates for further development. They inserted an octet...

  13. Hot Plasmids Spring 2024

    Type
    Blog Post
    ...in vivo screening of human promoters, the Zhang Lab found a short fragment of human IBA1 promoter (hIBA1a...-cell sequencing, and optical pooled screens in human cells. Addgene offers vectors for CROPseq-multi ...various contexts in vitro and in vivo, including in human retinal organoids and live mice. Importantly, REVeRT...AAV Vector System Neurodegeneration Collection updates   Multiplexed perturbation and decoding in pooled... New and notable Neurodegeneration Collection updates Last but not least, check out the new and noteworthy...Neurodegeneration Research Collection. This page is regularly updated with useful materials for research into Alzheimer...

  14. The PAM Requirement and Expanding CRISPR Beyond SpCas9

    Type
    Blog Post
    ...pyogenes Cas9 (3'-NGG) are abundant throughout the human genome, they are not always positioned correctly...double the targeting range of CRISPR/Cas9 within the human genome. Another variant, xCas9 3.7 has 7 mutations... reveals specificities of Cpf1 endonucleases in human cells. Nat Biotechnol 34:863–868 . https://doi.org...Genome-wide specificities of CRISPR-Cas Cpf1 nucleases in human cells. Nat Biotechnol 34:869–874 . https://doi.org...Originally published Nov 12, 2015 and last updated Aug 20, 2020. Cas9 can be used to modify any desired...Jennifer Tsang and Mary Gearing contributed to the update of this post. References Abudayyeh OO, Gootenberg... CRISPR Guide Browse All CRISPR Plasmids Find Validated gRNAs ...

  15. What's Your Organism? Expanding Genomic Tools via the NSF EDGE Program

    Type
    Blog Post
    ...(unless if you count normal human interaction for all of us studying human genes in test tubes and gels...Mello) These very cute birds learn their songs like humans learn speech. Finches must learn early, use auditory...existence, but most abundant cell on earth (twice the human biomass). The lab is developing a system using novel...research plans that went beyond obtaining “omic” data and had to include clear plans to connect Genotype... labs to rapidly disseminate protocols, methods, data, and tools that result from their work. Researchers...that tastes yucky (and is poisonous) for bird predators - birds eat a caterpillar once, vomit, and never...

  16. Serotype Testing AAV

    Type
    Collection
    ...Bryan Roth ) and direct EGFP expression from the human synpasin promoter. For information about each catalog...option is an economical choice that can be used to validate the performance of various serotypes before conducting...

  17. Distribution to Industry

    Type
    Collection
    ...CRISPR/Cas9-mediated saturation genome editing of human TP53 exon 5–8. Phagemid Synuclein VHH Immune Library...Addgene Parkinson's Research from The Michael J Fox Foundation Search Use Addgene's Search to find plasmids ...comprehensive end-joining ligation fidelity and bias data to predict high accuracy junction sets for Golden...for research in Parkinson's Disease; For target validation and drug design Antibodies Addgene distributes...produced in-house and undergo application-specific validation and quality control by Addgene as well as by ...

  18. Popular Retroviral Vectors and Their Uses in Scientific Research

    Type
    Blog Post
    ...technology pdCas9-humanized (Plasmid #44246) Expression of a catalytically inactive (dead), human codon-optimized...whereas lentiviral vectors are derived from the human immunodeficiency virus (HIV) genome. For research...Addgene: Follow our viral vector blog posts for updates on the most recent deposits, protocols, and tips...check www.genome-engineering.org for the most up-to-date information. Check our CRISPR guide and blog to ...

  19. Viral Vectors 101: Systemic Capsids

    Type
    Blog Post
    ...distinct interneurons in mice, non-human primates and humans.Nature neuroscience, 23(12), 1629–1636. https...evolve new systemic capsids for both rodents and non-human primates (NHPs). These engineered capsids have increased...adeno-associated virus; GRE, gene regulatory element; NHP, non-human primate. Many of the systemic capsids in our...rhesus macaque, are approximately more similar to humans than New World Monkeys and rodents. Image and caption...compared to AAV9 and AAV.MaCPNS2. Ex vivo macaque and human brain slices Several of the systemic capsids mentioned...pigtail (Macaca nemestrina) or rhesus macaque, and on human brain slices cultured in physiological conditions...outperformed the other capsids in the rhesus macaque and human brain slices. This capsid’s high transduction efficiency...

  20. Plasmids 101: The Wide World of Natural Plasmids

    Type
    Blog Post
    ...the tools of a millennia-old microbial war that humans only noticed last century! Nevertheless, the transfer...researchers have suggested they may have relevance to human health (Fogarty et al., 2024), and others have proposed...among the most numerous genetic elements in the human gut. Cell, 187(5), 1206-1222.e16. https://doi.org...routinely in biotechnology to transform plant cells. Degradative plasmids: microbial hardiness There are also ...organisms. To conserve energy, these specialized degradative capabilities are often tightly regulated to be...written by Jessica Welch in January 2017 and was updated by Emily P. Bentley in October 2024. References...
Showing: 81 - 100 of 302 results