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We narrowed to 87 results for: lentivirus expression plasmid

Showing: 81 - 87 of 87 results

  1. Zhang Lab CRISPR Page

    Type
    Collection
    ...tracrRNA: This plasmid contains three expression cassettes. To target a given site, the plasmid can be digested...nuclei in addition to sgRNA expression. This plasmid contains two expression cassettes, Cre recombinase... SpCas9 Plasmids The CRISPR/Cas system can be implemented in mammalian cells by co-expressing the S. pyogenes...., Science 2013). Additionally, we have a plasmid expressing SpCas9 alone without sgRNA. Full references...nickase) + single guide RNA: These plasmids contain two expression cassettes, a human codon-optimized ...sequence. 3. SpCas9 alone: This plasmid contains a single cassette expressing human codon-optimized SpCas9...

  2. Gamma-Retroviral Vector Guide

    Type
    Guide
    ... retroviral plasmids available at Addgene. Gamma-Retrovirus Versus Lentivirus Lentiviruses and gamma-retroviruses...Gamma-Retroviral Plasmid Elements Plasmid Type Element Delivery relative to transgene Purpose Transfer plasmid LTR ...main difference between lentiviruses and gamma-retroviruses is that lentiviruses are capable of infecting...that lentiviruses can infect a greater variety of cell types than retroviruses. Both lentiviruses and ...defined “generations” of plasmids, as lentiviral vectors do. For more about lentiviruses, see Addgene's lentiviral... of all gamma-retroviral plasmid components, see the Gamma-Retroviral Plasmid Elements table . In order...gamma-retroviral vectors, you need three plasmids (Figure 2): Transfer plasmid — contains transgene, sgRNA, or ...

  3. Plan Your Experiment

    Type
    Guide
    ...for details about lentivirus, see our Lentivirus Guide ) Stable, tunable expression of Cas9 and/or gRNA...activators exist, including the multi-plasmid SAM system. Select Expression System To use CRISPR, you will need...to the transient Cas9 expression. The table below summarizes the major expression systems and variables...Delivery method Expression System Components of System Application Mammalian expression vector Cas enzyme... (for details, see Plasmids 101: Mammalian Vectors ) Transient or stable expression of Cas9 and/or gRNA... or electroporation Transient expression of CRISPR components Expression decreases as RNA is degraded ...Additionally, many plasmids containing validated gRNAs are now available through Addgene. These plasmids contain ...

  4. CRISPR Plasmids - Empty gRNA Vectors

    Type
    Collection
    ... on the plasmid. gRNA plasmids that do not co-express a Cas protein require a separate plasmid that does...Vectors CRISPR Plasmids: Empty gRNA Expression Vectors Browse CRISPR Plasmids By Function Genome Editing Cut...maintained by the Doudna Lab . Plasmid Expression System Cloning Enzyme Co-expressed Cas9 Cas9 System Selection...can be used to express multiple gRNAs from a single plasmid with or without expression of Cas9. Highlighted...polycistronic lentiviruses. Can express Cas9 with up to 3 gRNAs. Frew pSQT1313 Mammalian Single plasmid for the...the expression of multipe gRNAs. Csy4 cleavable cassettes; requires expression of Csy4 (eg Plasmid 53369... sgRNA expression. Chen pCFD4-U6:1_U6:3tandemgRNAs Drosophila Single plasmid for the expression of two...

  5. Science Guides

    Type
    Guide
    ...different plasmids. Plasmids within a given library have the same backbone, but they express or target...about various scientific concepts related to our plasmid collection. Find links to the guides below. Antibodies...that are commonly used in research, including: Lentivirus Adeno-associated Virus (AAV) Adenovirus γ-Retrovirus...

  6. CRISPR Guide

    Type
    Guide
    ...multiple gRNAs using a single plasmid ensures that all gRNAs will be expressed in the same cell. This is ...need to generate lentivirus containing the entire library (Figure 8D). This lentivirus will then be used... co-infect with a Cas9-producing lentivirus or use a Cas9-expressing stable cell line. After applying ...tools require constitutive expression of the dCas9 fusion and/or expression of multiple fusions. The effects... DNA. This sequence is cloned into a gRNA expression plasmid but does NOT include the PAM sequence or ...Addgene's guide to using CRISPR plasmids in your lab, from knockouts to pooled library screening. Science...sequences . Once you’ve selected your target, you’ll express your gRNA and Cas9 protein, which will form a ribonucleoprotein...

  7. Immunology Research Plasmids and Resources

    Type
    Collection
    ... Plasmid Collections Immunology Research Immunology Research Plasmids and Resources You...Fluorescent Proteins Cancer Research Plasmids Gene Categories Plasmid Tables Additional Resources Mammalian...to the available plasmids for each gene. Please note that not all genes have plasmids available for request...and apoptosis. Plasmid Tables The gene names in these tables link to the available plasmids for each gene...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail...Addgene’s immunology collection contains plasmids for genes involved in innate and adaptive immune signaling...finds and attacks pathogens or infected cells expressing that antigen. Antibodies produced by B cells ...
Showing: 81 - 87 of 87 results