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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 86 results for: lentivirus expression plasmid

Showing: 81 - 86 of 86 results

  1. Plan Your Experiment

    Type
    Collection
    ...design. Addgene offers many plasmids containing validated gRNAs . These plasmids contain gRNAs that have ...depositors ). Browse empty gRNA expression vectors . Select Your Expression and Delivery System Once you ... your experiment) and the expression time (transient or stable). For plasmid and viral-based approaches...mammalian cells is to directly transfect expression plasmids encoding your Cas protein and gRNA, which...electroporation. Expression can be transient, or you can generate stable cell lines if your plasmid contains ...option, as they offer high expression levels, long-term and stable expression, are safe and easy to work...transfect, transduce, or may not express common promoters. Browse Cas9 plasmids for RNP delivery . As mentioned...

  2. CRISPR Plasmids - Empty gRNA Vectors

    Type
    Collection
    ... on the plasmid. gRNA plasmids that do not co-express a Cas protein require a separate plasmid that does...Vectors CRISPR Plasmids: Empty gRNA Expression Vectors Browse CRISPR Plasmids By Function Genome Editing Cut...maintained by the Doudna Lab . Plasmid Expression System Cloning Enzyme Co-expressed Cas9 Cas9 System Selection...can be used to express multiple gRNAs from a single plasmid with or without expression of Cas9. Highlighted...polycistronic lentiviruses. Can express Cas9 with up to 3 gRNAs. Frew pSQT1313 Mammalian Single plasmid for the...the expression of multipe gRNAs. Csy4 cleavable cassettes; requires expression of Csy4 (eg Plasmid 53369... sgRNA expression. Chen pCFD4-U6:1_U6:3tandemgRNAs Drosophila Single plasmid for the expression of two...

  3. Science Guides

    Type
    Guide
    ...different plasmids. Plasmids within a given library have the same backbone, but they express or target...about various scientific concepts related to our plasmid collection. Find links to the guides below. Antibodies...that are commonly used in research, including: Lentivirus Adeno-associated Virus (AAV) Adenovirus γ-Retrovirus...

  4. CRISPR Guide

    Type
    Guide
    ...multiple gRNAs using a single plasmid ensures that all gRNAs will be expressed in the same cell. This is ...need to generate lentivirus containing the entire library (Figure 8D). This lentivirus will then be used... co-infect with a Cas9-producing lentivirus or use a Cas9-expressing stable cell line. After applying ...tools require constitutive expression of the dCas9 fusion and/or expression of multiple fusions. The effects... DNA. This sequence is cloned into a gRNA expression plasmid but does NOT include the PAM sequence or ...Addgene's guide to using CRISPR plasmids in your lab, from knockouts to pooled library screening. Science...sequences . Once you’ve selected your target, you’ll express your gRNA and Cas9 protein, which will form a ribonucleoprotein...

  5. Plan Your Experiment

    Type
    Guide
    ...design. Addgene offers many plasmids containing validated gRNAs . These plasmids contain gRNAs that have ...depositors ). Browse empty gRNA expression vectors . Select Your Expression and Delivery System Once you ... your experiment) and the expression time (transient or stable). For plasmid and viral-based approaches...mammalian cells is to directly transfect expression plasmids encoding your Cas protein and gRNA, which...electroporation. Expression can be transient, or you can generate stable cell lines if your plasmid contains ...option, as they offer high expression levels, long-term and stable expression, are safe and easy to work...transfect, transduce, or may not express common promoters. Browse Cas9 plasmids for RNP delivery . As mentioned...

  6. Immunology Research Plasmids and Resources

    Type
    Collection
    ... Plasmid Collections Immunology Research Immunology Research Plasmids and Resources You...Fluorescent Proteins Cancer Research Plasmids Gene Categories Plasmid Tables Additional Resources Mammalian...to the available plasmids for each gene. Please note that not all genes have plasmids available for request...and apoptosis. Plasmid Tables The gene names in these tables link to the available plasmids for each gene...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail...Addgene’s immunology collection contains plasmids for genes involved in innate and adaptive immune signaling...finds and attacks pathogens or infected cells expressing that antigen. Antibodies produced by B cells ...
Showing: 81 - 86 of 86 results