We narrowed to 136 results for: max

1. Stabilized Bacterial Promoters: Constant Gene Expression at any Copy Number

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   ...further away. While constitutive promoters drove maximal gene expression near genomic origins of replication...

2. It’s Bioelectric! An Exciting Interdisciplinary Field of Research

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   ...activated by cyan light and exhibits an inactivation maximum of 590 nm light. Aurora and Phobos optogenetic ...

3. Viral Vectors 101: An Introduction to AAV

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   ...removed, resulting in recombinant AAVs (rAAVs) with maximum “trunk” space for genetic cargo. Typically, when...

4. The time and cost required to make a plasmid

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   ...time, there are a few options you can explore to maximize the use of both when making plasmids. Use repositories...

5. Celebrating 15 Years of Scientific Sharing

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   ...and software teams are working in lock step to maximize the services and features we can offer online ...

6. Plasmids 101: Modular Cloning

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   ...orientations and components within the system to maximize production potential. For example, the best promoter...

7. Mouse Modeling, Part 1: Genetically Engineered Mice

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   ...Outbred strains are strategically bred to maintain maximum genetic heterozygosity in the population, meaning...

8. Split Fluorescent Proteins for Studying Protein-Protein Interactions

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   ...complementation. TagBiFC (split HaloTag) provides maximum flexibility in color labeling with exogenous fluorophores...

9. High Complexity Golden Gate Assembly with NEB’s DAD

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   ... of fragments, you can also ask it to give the maximum or minimum number of fragments, or defined split...

10. Degrading DNA with Cascade-Cas3

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    ...comparable expression of all components of Cascade for maximum efficiency of the process. Various labs have designed...

11. Tips for a 1st time AAV user (by a Rookie AAV user)

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    ...pellet for the remainder of the cultures and later maxiprep only the cultures with intact ITRs. I use a spreadsheet...

12. Tips for Using BLAST to Verify Plasmids

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    ...that these regions are included in the search to maximize the alignment length. While this recommendation...

13. New Optimized Genome-wide CRISPRko, CRISPRi, and CRISPRa Libraries

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    ...Doench, John G., et al. "Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9...

14. CRISPR 101: RNA Editing with Cas13

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    ...database. They successfully edited 33/34 sites with a maximum of 28% editing efficiency in HEK293T cells, as ...

15. Getting to Know Expansion Microscopy

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    ...other SRM techniques and can be alleviated by maximizing label density. In some cases, nanobodies have...

16. Course-Based Undergraduate Research Experiences (CUREs)

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    ...he encourages faculty mentors.)  Collaboration Maximizes Reach Many new professors are wary of the time...

17. CRISPR 101: Making a Knock-In Cell Line

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    ...chromatid is likely to exist as a repair template. To maximize HDR events, ensure cells are actively cycling ...

18. Transferable Skills Guide: Identifying Your Transferable Skills

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    ...yourself so that you can grow them even more, get maximum satisfaction out your work, and be prepared for...

19. AAVs CREATed for Gene Delivery to the CNS and PNS

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    ...transactivator (tTA), is injected at a high dose to maximize the number of cells that are labeled. Part 2: ...

20. MXS Chaining

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    ...Structures Construct Fluorophore Excitation/emission maxima Tethering partner Subcellular localization 1 ...