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Showing: 81 - 100 of 552 results

  1. What’s in Your Tube?: A Quick Guide to Measuring DNA by Spectrophotometry

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    Blog Post
    ...sample. Ethanol carry-over from purification protocols is also a common concern, and while ethanol can reduce...residual guanidine thiocyanate. Phenol is a unique case, as pure phenol absorbs strongly at ~270 nm and...experiments. The usual suspects, such as proteins, phenol, or guanidine salts, exhibit their own characteristic...ones most likely to impact absorbance readings are phenol, guanidine salts, and detergents (Figure 1C-D; ...means that even samples with quite high levels of phenol (up to 600 ppm according to Loughrey and Matlock...yield “good” A260/280 and A260/230 ratios. Instead, phenol is more likely to shift the peak of your sample's...towards 270 nm. If your purification protocol uses phenol, then it is important to confirm that your sample...

  2. Addgene Welcomes John Doench to the Advisory Board

    Type
    Blog Post
    ...that had Platforms with a capital ‘P’ focused on technology ... has been critical to the Broad’s success ...biological questions, you need a tremendous amount of technology development alongside it.” Doench starting working...Perturbation Platform and encompasses a variety of technologies to functionally investigate the human genome...genome. Doench says he enjoys working on technology development under the Platform model at the Broad Institute...Institute because they are “not just developing technology for its own sake,” but work in true collaboration...history of RNAi left him a bit skeptical of the technology at the start of the CRISPR craze, but he says...justified… it has been just an incredibly useful technology.” He goes on to explain that, “everything that...

  3. Evolution of Lab Techniques

    Type
    Blog Post
    ...recombinant DNA technologies that are now commonplace in laboratories. As scientific technologies rapidly progress...computers, cell phones, and cars become more technologically advanced leaving earlier versions obsolete,...perusing classic papers or are brainstorming new technological innovations. Let’s take a look at three historical... use of Sanger sequencing. Building upon this technology, radiolabelled nucleotides were replaced with... DNA sequencing machines (9). Today, similar technologies, sometimes referred to as sequence DNA as it...properties of nucleotides (8). Compared to older technologies nanopore-based DNA sequencing machines are relatively... is projected that nanopore-based sequencing technologies could one day sequence an entire genome for ...

  4. How to Design Your gRNA for CRISPR Genome Editing

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    Blog Post
    ...Scientist at the Broad Institute, John Doench. CRISPR technology has made it easier than ever both to engineer...is true when designing gRNAs for using CRISPR technology – the “best” gRNA depends an awful lot on what...Gene knockout by NHEJ Gene knockout with CRISPR technology is usually accomplished by Cas9-mediated dsDNA... Created with BioRender.com. Two newer technologies offer an alternative to HDR for introducing ... another target C or A in the window. Another technology, prime editing (reviewed in Anzalone et al., ... although these are still early days for this technology, and the user may need to optimize numerous parameters...CRISPRa (activation) and CRISPRi (inhibition) technologies – a nuclease-dead Cas9 (dCas9) is directed near...

  5. PiggyBac-ing Through the Genome Editing Field

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    Blog Post
    ...please read on to learn more about this exciting technology from the folks at Transposagen. This post was...engineering approaches. Most importantly for this technology, however, is that the transposase also enables... with no known upper limit [1]. The piggyBac™ technology can be used for numerous applications, including...of interest. Fortunately, by combing nuclease technology with the piggyBac™ system, these challenges can... DNA using the piggyBac™ transposon selection technology using a site-specific nuclease to stimulate homologous...of using piggyBac™, you could use recombinase technology (Cre/loxP) to remove the donor cassette. While...summary, the piggyBac™ transposon is a powerful technology that may be used for a variety of research applications...

  6. RNA Extraction Without A Kit

    Type
    Blog Post
    ...acid-guanidinium-phenol solution that combines the homogenization solution and phenol addition of the ...extraction protocol with guanidinium thiocyanate-phenol-chloroform. In this method, samples are still homogenized...using cesium-chloride gradients, water-saturated phenol, sodium acetate, and chloroform are added to the...centrifugation (not ultracentrifugation!), the layers of phenol and chloroform separate, and RNA is retained in...aqueous layer is extracted and RNA can then be isopropanol precipitated. This method reduced the time it...laboratory situation or tissue collection method, so biotechnology companies have marketed several products that...of RNA Isolation by Acid Guanidinium Thiocyanate–Phenol–Chloroform Extraction. Analytical Biochemistry ...

  7. K. phaffii: Rising to the Occasion

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    Blog Post
    ...media change into methanol and peroxisomal autophagy can be induced by removing methanol from the media....protein animal feed. Due to increasing prices of methanol and advancements in other biomass production techniques...Preferred carbon source Glucose Glucose Glucose, methanol, glycerol Heterothallic mating Efficient... a methylotropic yeast, meaning it can utilize methanol as its sole source of carbon and energy (as well... its powerful promoter of a gene important for methanol usage. This gene promoter, AOX1, was co-opted ...all eukaryotic cells and peroxisomes are where methanol metabolism occurs in methylotrophic organisms....Riley, R., et al., Comparative genomics of biotechnologically important yeasts. Proc. Natl. Acad. Sci.,...

  8. Using CRISPR/Cas9 to Edit Disease Out of the Genome

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    Blog Post
    ...simple technology,” Doudna further explained. “That’s why we’re seeing adoption of the technology in different...University.  There can be no doubt that CRISPR/Cas9 technology has been a breakthrough for the genome-editing...CRISPR for complex genetic diseases As CRISPR technology becomes increasingly more cost effective and ...of those off-target mutations when using this technology. When CRISPR therapies are being given in vivo... “A glance at genome editing with CRISPR-Cas9 technology.” Current genetics. https://doi.org/10.1007/s00294... Khatibi et al. 2021.  “CRISPR Genome Editing Technology and its Application in Genetic Diseases: A Review...Review.” Current pharmaceutical biotechnology. https://doi.org/10.2174/1389201021666200621161610 4. Uddin...

  9. Cancer, Inflammation and Immunity - Harnessing the Body’s Defenses to Fight Cancer

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    Blog Post
    ...learn more about “... recent findings in basic immunology, cancer-immune cell interactions, cancer immunotherapy... conference was that Cancer Researchers and Immunologists are coming together to tackle the challenge ... from the conference which reflect how cancer/immunology researchers are preparing and carrying out this...inflammation and immunosurveillance have a look at our Immunology Research Plasmids and Resources. Studying the...the use of optogenetic tools is expanding to immunological research. Here the Zhou group presented Opto-CRAC...to calcium. We’d love to expand our cancer and immunology resources. If you have a great new plasmid you...Plasmids Browse Immunology Plasmids Find Kits for Cancer Research  ...

  10. CRISPR 101: Cytosine and Adenine Base Editors

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    Blog Post
    ... CRISPR-based cytosine base editing. Nature Biotechnology, 41(5), 663–672. https://doi.org/10.1038/s41587...activity and therapeutic application. Nature Biotechnology, 38(7), 892–900. https://doi.org/10.1038/s41587...concurrent adenine and cytosine editing. Nature Biotechnology, 38(7), 861–864. https://doi.org/10.1038/s41587...engineered Cas9-cytidine deaminase fusions. Nature Biotechnology, 35(4), 371–376. https://doi.org/10.1038/nbt...optimization and ancestral reconstruction. Nature Biotechnology, 36(9), 843–846. https://doi.org/10.1038/nbt...editors generated from TadA variants. Nature Biotechnology, 41(5), 686–697. https://doi.org/10.1038/s41587...editor with low off-target activity. Nature Biotechnology, 41(5), 673–685. https://doi.org/10.1038/s41587...

  11. Fluorescent Proteins 101: Monitoring Cell Mobility Using Fluorescent Proteins

    Type
    Blog Post
    ...thanks to these technologies but many questions remain. The use of other technologies such as optogenetics...could also be tracked by cell sorter giving immunologists the ability to count cells and statistically...different immune cells. These methods allowed immunologists to develop a spatiotemporal view of an immune... time. This technique was first Introduced to immunology by three papers in the journal Science in 2002...optogenetics and CRISPR/Cas9 will help immunologists create even better tools and models to further our understanding...infected with Toxoplasma gondii." The Journal of Immunology 182.10 (2009): 6379-6393. PubMed PMID: 19414791...interfollicular region of lymph nodes." Nature immunology 9.2 (2008): 155-165. PubMed PMID: 18193049.  ...

  12. Addgene-Seeding Labs Plasmid Grant: Accelerating Science Globally - 5 Scientists, 4 Countries & 3 Continents!

    Type
    Blog Post
    ...insecticidal proteins to the development of renewable biotechnology products and biosensors for herbicides as well...a bacterial expression system for renewable biotechnology products Dr. Idah Sithole-Niang, University ... Lecturer and a Plant Molecular Biology and Biotechnology expert. In order to preserve Cassava yields ...Robertson aims to make use of the CRISPR/Cas9 technology to engineer plants that are able to destroy DNA...Associate Professor (Right), focuses on molecular biotechnology for improving expression of nutritionally and...Bao Ton (center) is interested in applying gene technology to bacteria and plants. Her research involves...water contaminants and to subsequently develop technologies to protect fresh water repositories in Mexico...

  13. 27 Hot Plasmids from 2016

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    Blog Post
    ...activity mapping with STARR-seq   Although many technologies have been developed for the study of enhancer...available from Addgene in both human and fly versions. Arnold, et al. Science. 2013. PubMed PMID: 23328393 Wu... and mutant library generation in several biotechnologically and clinically relevant bacterial species...SnoopCatcher The development of recombinant DNA technology has allowed molecular biologists to fuse fragments..., such as the food and beverage industry, the bioethanol industry, and the pharmaceutical industry, depend...through Addgene. Stovicek, et al. J Ind Microbiol Biotechnol. 2015. PubMed PMID: 26376869 Jensen, et al. FEMS...modification in Caenorhabditis elegans CRISPR/Cas9 technology has revolutionized genome editing in multiple...

  14. Luminescent Imaging with Nano-lanterns

    Type
    Blog Post
    ... Osaka University have developed Nano-lantern technology. Nano-lanterns contain a Renilla luciferase variant...but it has precluded widespread adoption of the technology. In 2012, Saito et al. described the first Nano-lantern...luciferase (RLuc8) with Venus. This luminescent technology is based on the principle of bioluminescence ...constructs use the brightest characterized luciferase, NanoLuc, and its substrate furimazine. In addition to the...optogenetic system. Biosensors based on Nano-lantern technology are also very robust, with an eNL-based calcium...ratio. Despite the many benefits of Nano-lantern technology, there are a few drawbacks associated with luminescent... not be appropriate for use with Nano-lantern technology. Multicolor imaging using Nano-lanterns requires...

  15. Adeno Associated Virus (AAV) for Cell and Gene Therapy

    Type
    Blog Post
    ...Harshana S De Silva Feelixge. Gene therapy technologies hold great promise for improving or potentially...Rapid advances in next generation sequencing technologies have allowed scientists to quickly identify ...Almost all patients have achieved persistent immunological reconstitution with a normally functioning T...been challenging. A variety of vector delivery technologies have been investigated including lentiviruses... delivery tools such as nanoparticles. These technologies can have serious adverse effects, and their ...discovered frequently and recent advances in AAV technology have enabled us to generate new and refined forms...consideration, recent advancements in various AAV technologies have vastly improved AAVs’ potential to become...

  16. Transgenic Organisms, Cas9 Gene Drives, and Appropriate Safeguards

    Type
    Blog Post
    ... post was contributed by Kevin Esvelt, a Wyss Technology Development Fellow at the Wyss Institute and ...widespread trust in scientists to develop new technologies with care and humility. Nothing would damage...could not be trusted to handle such a powerful technology responsibly. An accidental release would be particularly...Finally, gene drives are an inherently collective technology: because they necessarily alter the shared environment...Thank you to our guest blogger! An inventor of technologies that harness evolution, Kevin Esvelt studies...ensuring that gene drives and other collective technologies are developed safely and transparently.  For...101: a blog series on the basics of CRISPR-Cas technology CRISPR Resources from Addgene   ...

  17. Capturing a Moment with Marc Zimmer’s “The State of Science”

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    Blog Post
    ...implications of using these technologies, but also the rapidity of the technologies’ adoption by the scientific...amongst the Nobel prize winners for developing the technology. Though these case studies are “old,” they lead... learning addresses the dilemma of creating a technology that could eventually replace scientists themselves...Addgene’s role in the rapid dissemination of these technologies, proposing that “Addgene, with its easy, fast...CRISPR-Cas plasmids, has been a key driver of the technology.” As an Addgene vet of 12 years, I’m very familiar...decline in the competitiveness of U.S. science and technology.” This last section of the book, Future Science...

  18. The CRISPR Software Matchmaker: A New Tool for Choosing the Best CRISPR Software for Your Needs

    Type
    Blog Post
    ...in the malaria community for the better (Nat. Biotechnol., 2014). Given the timing, it shouldn’t be a ...yet. As a genome editing tool, the CRISPR/Cas9 technology has been surrounded by a whirlwind of research... sgRNAcas9. Top tools for transferring CRISPR technology to a new organism: For obvious reasons you can...developed with the purpose of applying CRISPR technologies to new organisms and will help you whether you...Report your findings to help everyone else. CRISPR technology has reached into so many different communities...falciparum using the CRISPR-Cas9 system." Nature biotechnology(2014). PubMed PMID: 24880488. 2. Hsu, Patrick...specificity of RNA-guided Cas9 nucleases." Nature biotechnology 31.9 (2013): 827-832. PubMed PMID: 23873081....

  19. Finding Your Science Policy Path

    Type
    Blog Post
    ...contributed by Steph Guerra, a AAAS Science and Technology Policy Fellow at the Veterans Health Administration...roles and my current work as a AAAS Science and Technology Policy Fellow (STPF) at the Veterans Health Administration...these resources compiled by the AAAS Science and Technology Policy Fellowship Program to learn more about...could understand how to make new treatments and technologies accessible to patients. And what better place...guest blogger Steph Guerra, a AAAS Science and Technology Policy Fellow at the Veterans Health Administration... Guerra, PhD, is currently a AAAS Science and Technology Policy Fellow at the Veterans Health Administration...particularly interested in driving scientific and technological innovation that is accessible and equitable ...

  20. Sequencing Options for CRISPR Genotyping

    Type
    Blog Post
    ...CRISPR-Cas nucleases in human cells." Nature biotechnology 31.9 (2013): 822-826. PubMed PMID: 23792628....on-and off-target genome editing." Trends in biotechnology 33.2 (2015): 132-140. PubMed PMID: 25595557....RNA-programmed Cas9 nuclease specificity." Nature biotechnology 31.9 (2013): 839-843. PubMed PMID: 23934178....off-target cleavage by CRISPR-Cas nucleases." Nature biotechnology 33.2 (2015): 187-197. PubMed PMID: 25513782....of zinc-finger nuclease specificity." Nature biotechnology 29.9 (2011): 816-823. PubMed PMID: 21822255....integrase-defective lentiviral vectors." Nature biotechnology 33.2 (2015): 175-178. PubMed PMID: 25599175....breaks induced by engineered nucleases." Nature biotechnology 33.2 (2015): 179-186. PubMed PMID: 25503383....
Showing: 81 - 100 of 552 results