... et al. Although I was able to knock-in a 2xFLAG epitope into my gene of interest, I encountered low efficiency...the model organisms which I use to study gene regulation during developmental processes, CRISPR/Cas9 allows...CRISPR editing in C. elegans improves efficiency  I was fortunate – or unfortunate enough, depending on... alternate approaches. Being a “lazy” geneticist, I developed a co-selection approach relying on repair...courtesy of Jordan Ward, UCSF. In 2015 (Ward, 2015), I demonstrated that selection for repair of a temperature-sensitive...Nitschko V, Obermaier C, Philippou-Massier J, Wieland I, Gaul U, Forstemann K (2014) Efficient chromosomal...selective marker to remain in the edited animal. Development of CRISPR/Cas9 editing strategies in C. elegans...

...but it works for me. I can often remember which paper I need to open, but when I can’t it’s an easy search...organization system that works for you. I have a folder where I dump all of my papers and they all have... it leads you to the same conclusion. Personally, I like papers that can end with some sort of model (...Scientific Manuscript by Maaike Pols, PhD, a Developmental Editor at F1000Research  ...

...management. I needed it when balancing a handful of demanding courses, a capstone paper I really wanted...creativity; I have developed these skills through school, jobs, and volunteer work, and I guarantee you...time work, bills, (at times) a social life and rest. I need it just as much in my current role as Customer... and Operations Manager at Addgene. In this role, I balance my daily tasks, meet cross-team project commitments...graduate school. These skills will be useful anywhere I work in the future; they are transferrable across ...interested in nonprofit management and international development.       Additional Resources on the Addgene Blog...

... the scientists were waiting. I look forward to sharing new developments on this project and on all the...world. Now that the service is successfully launched, I would like to thank some of the people and organizations...have such an impact on scientific materials sharing. I would also like to recognize a few scientists who ...practices and encouraging us to explore new directions. I would like to thank them for their time, dedication...Addgene Blog and our expanding website resources. I hope this gave you the impression that it took a lot...

... right way?  First of all, I want to put to rest a common misconception. I find personal bias often unintentionally...how they prefer to consume information. Personally, I would much rather read the instructions than watch...the box. There are so many ways to communicate and I wholeheartedly encourage experimentation. You never...What resources do you have?  No matter the project, I can guarantee you that either resource or time restrictions...re happy with. Know if you’ll need specialized equipment and/or expertise from the start, or if you can... media formats. Cognitively demanding format. Equipment, expense and expertise needed can increase exponentially...

... funding. I’ve been aware of these problems since I began my own career over 20 years ago, and I’m sure...misrepresent science, or misuse science, and that is what I would call bad science.”  The three chapters in this...describes the detection of gravitational waves--a story I was much less familiar with as a biologist--as an ... to be foremost in readers’ minds. Six months ago I would have nodded my head knowingly and appreciatively...slightly dated by next week.   A stepping stone When I discussed The State of Science with the five Addgene...challenges you face in your career.  Acknowledgements: I would like to thank fellow Addgenies Joanne Kamens...identification of Green Fluorescent Protein (GFP) and its development into an essential tool in biology also falls ...

...animals than i-GONAD and is a more technically difficult process. Overall, the main drawback of i-GONAD is...Diagram of ovary and oviduct anatomy with the equipment used for i-GONAD. A micropipette is used to inject ...responsible for Easi-CRISPR have also recently developed i-GONAD as a simple method for delivery of genome editing...germline. In contrast to standard microinjection, i-GONAD is performed directly on pregnant female mice...Ohtsuka et al. compared traditional microinjection and i-GONAD knock-in efficiency using a small ssDNA, and... with a long ssDNA donor was less efficient using i-GONAD (15%) vs. microinjection delivery (25-67%). ....2017.153 PubMed PMID: 29266098 Ohtsuka, Masato, et al. i-GONAD: a robust method for in situ germline genome...

...were very anxious at this point. Here's the message I shared with the Addgenies on March 11, 2020 after ...figure out how to deliver when labs finally opened.  I was awed by the teamwork and initiative it took to...and the subsequent fluctuations in distribution. I don’t have space in this blog to call out every Addgene...new projects on track to succeed at the same time. I began approaching science funding organizations to...t raise money except occasionally for capital equipment or resources needed to launch a new service or...

... week, I felt overwhelmed. Jumbled thoughts such as “I have no idea how to write a grant!”, “I don’t have... mind. Moving forward from that first experience, I learned the details of grant writing, volunteered ...Research strategy The Research Strategy highlights i) the significance and innovation of the project and...chance to delegate funds to supplies, personnel, equipment, travel, etc. As a first time budget writer, you...

...additional mentors. I found this beneficial for my projects, my training, and when I went on the job market...: What is the most exciting story I can tell with my data?  Can I make better slides that more effectively...signing up for and how can you do it well? Below, I briefly describe some tips for a successful postdoc...unpublished information and reagents. Part of the reason I got my first faculty position was because the search...asked someone at my institute about me, even though I had not asked this person to write a letter of reference... improves your perspective like intense exercise. I highly recommend running or biking or something that...this person cares about your science and your development. Taken together, all of this means that you need...

...writing habit into beautiful graphs! I like to look at these graphs when I need that extra push to stick with...suggestions on how to make your writing less complex. While I don’t think you should accept all of the suggestions...School podcast episode 2 "Organizing Literature" : I found out about Zotero here Dear Grad Student podcast...use in black. This paper talks more about the development of the De-jargonizer.   Academic Phrasebank  ...

...creative thinking. I won’t go through all nine here, but I’ll discuss a few that I frequently observe ...optimize virus production with roller skates—though I could get around the lab faster that way— but the ... and prevent groupthink? Here are some ideas that I have found helpful in my team brainstorming sessions...may feel they can share opposing opinions as well. I am sure we have all experienced a meeting where one...protocols and even our Lab Inventory Management system. I hope these tips help you to see that the creative ...form at any time and a member of our Business Development team would review it. This survey generated 26...

...., Woodard, K., Cepko, C., Guerin, K. I., Rego, M. A., Ersing, I., Bachle, S. M., Kamens, J., & Fan, M...recently released Viral Vectors 101 2nd edition — I was incredibly excited when we picked our viral vector... Viral vectors are, after all, a delivery method. I would be remiss to talk about them without looking...from eBooks to guides to protocols, which was why I was so excited to delve into this data as we updated...Campus on the GENIE website.   Bibliography  Ersing, I., Rego, M., Wang, C., Zhang, Y., DeMaio, K. H., Tillgren...process have shaped and supported our growth and development. We’ve shared what we’ve learned, through manuscripts...

... can help you. You Can Talk to Your Editors I wish I had been more cognizant of this during my PhD....the Future of Research (FOR) Boston Symposium 2015. I personally participated as a member of the Career ...ll point out some of the highlights and takeaways I got from the symposium. The goal of the Future of...achieve success in their careers. The take-away message I took from the career panel was that there are many... making it more easily digestible/available. What I viewed as the most important take-away from this session... to trainees navigating the waters of career development. Publishing Panel The Early Career Researchers...

...the classroom. I had the opportunity to talk with Provost recently about his development of what is now...planarian regeneration into one of my own courses, I was excited to hear about how he developed his program... like an exciting opportunity for your classroom, I highly recommend you try implementing one. It's likely...learning in a year-long biochemistry laboratory: Part I – guided inquiry – purification and characterization...progenitor cell maintenance in planarians in Developmental Biology (Kimball et al., 2020). Benefits and... pathway to diversify science. Evolution and Development. 24(5):127-130. Kimball, C., Powers, K., Dustin... progenitor cell maintenance in planarians. Developmental Biology. 457:119-127. Knutson, K., Smith, J....

.... However, I have a list of what I really need in order to reach my research goals. Would I be able to...include: equipment, reagents, animals, glassware, and even people. When developing a budget, I suggest ...of the major equipment that you use in your current lab first. Generally, major equipment can be defined...requires specialized equipment, try finding institutes that offer shared equipment that you can use at ...defined as equipment with capital costs greater than $5,000 but this can depend on the institution/funding...funding source. Major equipment includes large centrifuges, sequencers and analyzers, and any ultra sensitive...sensitive equipment (RT-PCR, Microscopy, Fluorescence Scopes, Flowcytometers, etc.). After identifying all...

...thing. Nevertheless, the models are compelling, and I encourage you to hold one. Why do we need physical...physical model. Until then, you can only wonder what I am talking about. How do researchers use physical ...they will almost invariably say something like “Oh,…I didn’t quite appreciate that….” For example, when ...polypeptide emerges from the exit channel.   He then said “I hadn’t realized how perfectly flat the complex is ...an initial solution to this problem with the development of Byron’s Bender – a mechanical device that ...molecular structures for use in professional development programs for bioscience educators. Using these...

...ion-channels and pumps in developmental biology, regeneration studies, and cancer biology. I was first introduced...introduced to bioelectricity when I attended the Developmental Bioelectricity satellite symposium at the 2019...meeting I had an increased appreciation for the role of ion channels and pumps in developmental biology...2019 Society for Developmental Biology meeting in Boston. The atmosphere was electric! After attending...whole scientific community. Over the years, developmental biologists and cancer researchers are beginning... decision-making in somatic cell networks. “Developmental bioelectricity is an extremely exciting emerging... "The body electric 2.0: recent advances in developmental bioelectricity for regenerative and synthetic...

...need to push the boundaries of discovery. As I look ahead, I am excited to continue to grow and innovate...Houston, D. W., Polinski, N. K., Meijers, R., Levey, A. I., Saper, C. B., Errington, T. M., Turn, R. E., Bandrowski...science. Want to learn more about our latest developments? Subscribe to the Addgene newsletter. References...

...  It was with a sense of relief that I attended the amazing, postdoc-driven Future of Research Conference...supporters from all of the research centers in Boston.  I was relieved because it finally feels to me like the...the Workforce, Metrics and Incentives and Funding.  I can’t wait to see what solutions these talented postdocs...progress reports information on the Individual Development Plans (IDPs) for the graduate students and postdocs...