Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

Information

In Category

We narrowed to 393 results for: prep

Showing: 81 - 100 of 393 results

  1. Retrograde AAV viral preps

    Type
    Collection
    ...eligible AAV plasmids and we’ll make the viral vector prep you need. Or fill out our form to let us know what...what you would like to see available in viral prep format. Please note this does not guarantee viral vector...final viral vector preparation. In some cases, in vivo testing of these viral preparations was performed by...Addgene is producing ready-to-use viral vector preparations in a retrograde serotype from the Alla Karpova...David Schaffer laboratories. These viral vector preparations were produced with the rAAV2-retro helper plasmid...DREADD Roth 154867 pAAV-hSyn-fDIO-hM4D(Gi)-mCherry-WPREpA Syn Inhibitor, Flp-dependent DREADD Gether 154868...154868 pAAV-hSyn-fDIO-hM3D(Gq)-mCherry-WPREpA Syn Activator, Flp-dependent DREADD Gether 83896 pAAV-hDlx-GiDREADD-dTomato-Fishell...

  2. Viral Production

    Type
    Collection
    ...order to generate high quality preparations. After production, all virus preps are titered and subjected to...typically prepared using endotoxin-free plasmid purification kits. Resources Browse lentiviral preps available... was a gift from Karl Deisseroth (Addgene viral prep # 55632-AAVrg). pAAV-CAG-FLEX-rc [Jaws-KGC-GFP-ER2...Jaws-KGC-GFP-ER2] was a gift from Edward Boyden (Addgene viral prep # 84445-AAVrg). Electron Microscopy The ratio of...arrowhead). Scale bar = 100 nm. Resources Browse AAV preps available from Addgene. After receiving your virus...plasmid encoding adenoviral helper sequences. AAV preparations are purified by iodixanol gradient ultracentrifugation...purity and titers adequate for in vivo studies. Preparations are then aliquoted and stored at -80 °C. Titer...

  4. pSiM24: Simplifying Plant Genetic Engineering

    Type
    Blog Post
    ...in other systems, binary vectors are cloned and prepped in E. coli. However, they also need to be able ...plant infection, as well as plasmid yield during preparation in E. coli. Addgene depositor Indu Maiti has ...

  5. Important Considerations When Using AAVs

    Type
    Blog Post
    ...cell populations. Find Ready-to-Use Retrograde AAV Preps at Addgene 2) Comparison of genetically engineered... Vectors for Your Research Get Ready-to-Use AAV Preps Browse All of Our Viral Plasmids and Resources ...

  6. DNA Service - Cloning Grade DNA

    Type
    Collection
    ...cloning grade DNA order? For each cloning grade DNA prep, the plasmid sequence has been validated by next...guarantee that Addgene will make cloning grade DNA preps of this plasmid available, but helps to inform us...

  7. Neurodegeneration Research Collection

    Type
    Collection
    ...Biotechnol. 2023 May 22. See More AAV Viral Preps Find AAV viral preps for systemic delivery of viral particles... Info Plasmid Collection CRISPR Tools AAV Viral Preps iPSC Differentiation Factors Antibodies Fluorescent...Noteworthy: AAV.rTH.PI.Cre.SV40 ready-to-use viral prep useful for dopamine research. CBA-driven, Cre-dependent...

  8. Genomic Deletions in Mammalian Cell Lines

    Type
    Collection
    ...a maxi-prep culture. Prep size may be scaled based on the required DNA yield. Perform maxi-prep for each...colonies and inoculate into a mini-prep culture. Perform mini-prep for each sample and sequence each colony...relevant antibiotic. Select multiple colonies, mini-prep each one, and subject each clone to Sanger sequencing...oligos at a concentration of 100 μM in ddH 2 O. Prepare a 10 μl reaction mix for each guide and its reverse...using a Golden Gate assembly cloning strategy 3 . Prepare a 50 μl reaction mix: 100 ng circular pX330 vector...Activated Cell Sorting (FACS) of Transfected Cells Prepare cells for FACS by filtering them through a 50 μm...plasmid DNA from bacterial colonies using the Qiagen Miniprep Kit. Zhang S, Cahalan MD. J. Vis. Exp...

  9. AAV Molecular Tools

    Type
    Collection
    ...eligible AAV plasmids and we’ll make the viral vector prep you need. Or fill out our form to let us know what...what you would like to see available in viral prep format. Please note this does not guarantee viral vector...

  11. Serotype Testing AAV

    Type
    Collection
    ...eligible AAV plasmids and we’ll make the viral vector prep you need. Or fill out our form to let us know what...what you would like to see available in viral prep format. Please note this does not guarantee viral vector...

  12. Addgene Packaged on Request: Scope of Service

    Type
    Collection
    ...limited use label licenses (LULLs). Titer For AAV preps produced through our Packaged on Request service... we may expect lower titers for a particular AAV prep, mostly likely due to one of the following reasons...

  13. Brain Armamentarium

    Type
    Collection
    ...Plasmids ID Plasmid Description PI Viral Preps ID Viral Prep Description PI (Cargo) PI (Capsid) 214869... and network function. Browse plasmids or viral preps created with support from the BRAIN Armamentarium...

  14. University of Florida Serotype Testing Panel for the Eye and Brain

    Type
    Collection
    ...We provide high quality AAV preps from select plasmids in the repository. Browse the University of Florida...eligible AAV plasmids and we’ll make the viral vector prep you need. Or fill out our form to let us know what...what you would like to see available in viral prep format. Please note this does not guarantee viral vector...sequencing of the final viral vector preparation. These viral vector preparations were produced with the pTR-UF11...Service , Addgene is producing ready-to-use viral preparations from the University of Florida for enhanced ...-UF11 plasmid ( Addgene #157970 ). Viral preparations are formulated in one of the following three buffers...

  15. AAV for Neuronal Tracing

    Type
    Collection
    ...eligible AAV plasmids and we’ll make the viral vector prep you need. Or fill out our form to let us know what...what you would like to see available in viral prep format. Please note this does not guarantee viral vector...

  16. The Importance of High Titer for AAV Transductions

    Type
    Blog Post
    ...Resources on Addgene.org Find Ready-to-Use AAV Preps Find Lab Protocols for Work with Virus Watch Our...Make sure your cells are as healthy as can be to prepare them for the arduous process of generating viral...

  19. Antibody Production

    Type
    Collection
    ...to generate high quality preparations. Following production, all antibody preps are titered and undergo...Sanger Sequencing Purified recombinant antibody preps contain low levels of co-purified plasmid from transfection...Innovation Antibody Collection webpage. Antibody preparations developed at IPI may differ from Addgene antibodies...repeated freeze thaw of antibodies and suggests preparing single-use aliquots before storing at -20 °C. ...
Showing: 81 - 100 of 393 results