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We narrowed to 395 results for: promoter

Showing: 81 - 100 of 395 results

  1. Scientific Reproducibility - Focusing on Solutions at the Minisymposium on Reproducibility

    Type
    Blog Post
    ...resources and information about organizations promoting reproducibility in this booklet (which was also...line we may see further adoption of policies that promote reproducible publishing practices. One example ... also hopefully see a shift toward hiring and promotion practices that focus on reproducibility and ensuring... Including descriptions of many organizations promoting reproducibility List of Tools for Reproducibility...

  2. Hot Plasmids: Spring 2025

    Type
    Blog Post
    ...new backbones special: Strong expression: A CMV promoter drives robust gene expression in mammalian cells...creation a breeze. Modular by design: Key elements (promoter, tag, polyA site) are flanked by unique restriction... both new and known regulators at the FOS gene promoter and the MYC locus (Figure 5). Figure 5...identification of heat-shock proteins at the FOS gene promoter using TurboCas, streptavidin immunoprecipitation...

  4. CRISPR 101: Ribonucleoprotein (RNP) Delivery

    Type
    Blog Post
    ... cells where common eukaryotic promoters (such as CMV or EF1A promoters found in many CRISPR plasmids)...transfection or viral transduction     Appropriate promoters must be chosen for both Cas9 and gRNA expression...

  5. Fluorescent Tagging of Endogenous Genes with SapTrap

    Type
    Blog Post
    ... site accepts the sgRNA target sequence for U6 promoter expression and the second site accepts the homology... a direct fusion of the desired gRNA to the U6 promoter, and a second plasmid containing the homology ...regions of homology to the gene of interest to promote homology directed repair after a CRISPR/Cas9 induced...

  6. Viral Vectors 101: Chemogenetics

    Type
    Blog Post
    ... targeting expression involve tissue specific promoters, AAV serotypes, and the ever-growing variety of...channel, such as the serotonin 3 receptor, will promote depolarization in neurons. Alternatively, combining...anion channel, such as the glycine receptor, will promote hyperpolarization. PSAM ligands The advantage of...

  7. Your Lentiviral Plasmid FAQs Answered

    Type
    Blog Post
    ...majority of transfer plasmids use the weak viral LTR promoter to drive expression of the gene of interest. Consequently...have a chimeric 5'LTR that includes a CMV or RSV promoter as well as a portion of the 5'LTR. Including a...2nd-generation system because the wildtype 5'LTR promoter requires Tat to function. Third-generation plasmids...

  8. Save Time with Transient Plant Leaf Transformations

    Type
    Blog Post
    ...are therefore good temporal reporters to measure promoter activity, for example. Useful positive controls...dark after the transformation. Use introns Plant promoters can be recognized or leaky in Agrobacterium. This...of the GUS gene with the commonly used CaMV 35S promoter in Agrobacterium. This can be easily avoided by...

  9. Isolating Genomic Regions of Interest with the CRISPR System

    Type
    Blog Post
    ... repression of transcription through binding a promoter region to inhibit association of transcriptional...targeting their genomic locus of interest, the promoter region of the transcription factor IRF-1. Immunoprecipitation...lab found a microRNA (miR483) that binds to the promoter of the growth factor IGF2 open reading frame to...

  10. Negative Can Be Positive: Open AAV Data with Addgene

    Type
    Blog Post
    ...under-informed with respect to how different serotypes, promoters, and injection methods are best suited to their...containing an expression cassette with an ubiquitous promoter to express GFP in a group of young rats. We found...other important factors such as what is the best promoter to use, or if and how much the spread of the virus...

  11. Plasmids 101: Shuttle Vectors

    Type
    Blog Post
    ... actually express your gene of interest? Many promoters are species-specific, so you’ll need to design...However, you do still need a species-compatible promoter for expressing your selectable marker. Otherwise...Plasmids 101: Mammalian Vectors Plasmids 101: The Promoter Region Resources on Addgene.org Collection of...

  12. Viral Vectors 101: Systemic Capsids

    Type
    Blog Post
    ...transgene expression. Gene regulatory elements [promoters and enhancers, miRNA target sites, and recombination...over systemic delivery. Otherwise, cell-specific promoters and enhancers (Mich et al., 2021) can help restrict...optogenetic and chemogenetic tools. Dual expression Promoters, miRNAs, enhancers, and switches all take up precious...particular experiment is determined by the capsid and promoter/enhancer strengths in the cell type of interest...candidate regulatory elements such as enhancers and promoters for cell type-specific targeting (Chan et al.,...when paired with appropriate cell type-specific promoters or Cre lines. Spatial transcriptomics suggests...AAV-PHP.eB, when paired with the ubiquitous CAG promoter, is relatively biased toward L5 and inhibitory...

  13. New and Upcoming Viral Vectors - Spring 2019

    Type
    Blog Post
    ...hM4D(Gi)-mCherry (CD68 is a  microglia/macrophage promoter) 121539  AAV5  pOTTC1596 - pAAV SYN1 HA-hM3D... GP-AAV-CAG-FLEX-jGCaMP7f-WPRE AAV1 with a CAG promoter exhibits high specificity for Purkinje neurons...

  15. Lentivirus Production

    Type
    Protocol
    .... Pro-Tip Different brands and lots of FBS can promote or inhibit transfection. Test a variety of brands...

  16. Colony Formation Titering Assay

    Type
    Protocol
    .... Pro-Tip Different brands and lots of FBS can promote or inhibit transfection. Test a variety of brands...

  18. CRISPR 101: Which Cas9 Do I Choose for My CRISPR Experiment?

    Type
    Blog Post
    ...dCas9 along with transcriptional repressors to the promoter region of the gene of interest. dCas9-based repressors... and a dCas9-KRAB fusion, both targeted to the promoter region. Cas9 activators include Cas9 fused to ...the Suntag array of factors, all targeted to the promoter region.   Ok, I have a Cas9! What do i do ...

  19. New Acoustic Reporter Genes: Ultrasound Imaging of Gene Expression

    Type
    Blog Post
    ...expression systems in E. coli. The arabinose-inducible promoter pBAD and a low-copy number origin of replication...integratable cassettes, both with doxycycline inducible promoters (Fig. 2a). The first cassette contains the major...constitutively-expressed mARG plasmids driven by CMV promoters. Labs that are new to the mARG expression are ...

  20. 5 Reasons to Use Reddit for Science Communication

    Type
    Blog Post
    ...downside to the AMA from my end as an organizer/promoter was that I didn’t have the direct link to send...of the AMA. This made it a little difficult to promote the AMA on social media beforehand (I just sent...sent people over to r/Science in the initial promotion as a work around… there’s plenty of great stuff there...
Showing: 81 - 100 of 395 results