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Follow this protocol to produce adeno-associated virus (AAV) in HEK293 cells.

This post describes the design of shuttle vectors, which are designed for replication, selection, and expression in more than one host species.

Transient plant leaf transformation can save you time... months in fact. Learn more about this method and get some tips and tricks for designing your transient plant leaf transformation experiment.

Learn how you can use MXS-Chaining to generate complex, multicistronic plasmid constructs.

Tim Miles, the guest speaker at our recent AAV webinar, and our webinar panel of experts, is finishing up their Q&A session.

Get a behind-the-scenes look at Addgene's new search engine.

Learn all about how to use a new plasmid for RNAi in C. elegans, tools for proximity labeling, RNA-based methods for detecting viral outbreaks, and tools for characterizing protein domain binding specificities.

Epigenetic modifications are an additional layer of control over gene expression. Targeted epigenetic modification is possible using CRISPR tools from Addgene!

Open science resources like Addgene's Data Hub increase reproducibility, reduce validation and optimization timeframes, and drive science forward.

Curious about what an ITR is and why it's so important for an AAV? Learn all about inverted terminal repeats in this Viral Vectors 101 post.

Getting start with mouse models? Here, we break down how to cross mice to create double knockout lines and Cre-lox lines.

Addgene reached its 15 year anniversary on January 8, 2019. In this blog post, find the top 15 requested plasmids since our founding. Many of these include lentiviral or CRISPR tools!

Get started with Gibson Assembly Cloning!

Enjoy 12 new CRISPR articles from Addgene's 12 Days of CRISPR!

Curious about systemic capsids? Looking for one to test in your experimental system? This 101 is an introduction to systemic capsids uses and types!

Learn about a variety of AAV encoded calcium biosensors available from Addgene.

Learn how you can use plasmids to teach students about functional genomics with your own "Pathways Over Time" project.

Find information on CRISPR mammalian expression systems and delivery methods. Use the table to quickly compare between the methods.

We sit down with SciComm experts, Vini and Amy, from the graduate student group Science in the News.

Trying to decided between a master's or a PhD? Here's five questions to ask yourself.