We narrowed to 130 results for: tet on

1. Sleeping Beauty Awakens for Genome Engineering

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   ...and one set of vectors for inducible expression (pSBtet derivatives). The constitutive and inducible expression...

2. Management for Scientists: What Makes a Good Manager Anyway?

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   ...managing people: Skills for New Managers by Morey Stettner Successful Manager's Handbook: Develop Yourself...

3. Hot Plasmids and Viral Preps - September 2021

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   ...the expression of fluorescent proteins and oxytetracycline enzymes. Next, this system was used to recreate...

4. Plasmids 101: A Brief History of Plasmids and an Improved eBook!

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   ...experiment. Dr. Cohen and colleagues treated a tetracycline resistant plasmid, pSC101, and a newly developed...

5. Hot Plasmids - September 2019 - CRISPR, Auxin Inducible Degradation, MerMAID Channelrhodopsins, and Genome Looping

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   ...latter experiments, such as transcription factors tethered to dCas9, require long time scales with constitutively...

6. New and Upcoming Viral Vectors - June 2019

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   ...  AAV5 ppAAV-CKIIa-stGtACR1-FusionRed New tetracycline transactivators Plasmid Serotype Name 99120...

7. Improving Transduction Efficiency with Exosome AAVs

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   ...Exosome-Associated AAV by Overexpression of the Tetraspanin CD9." Molecular Therapy-Methods & Clinical Development...

8. Fluorescent Protein Travel Awards - FLiPs and Fluorescent Protein Biosensors

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   ...hypothesized that she could make enzymes controllable by tethering them to Dronpa, a fluorescent protein known to...

9. Pooled CRISPR Libraries Offer Genome-Wide Control for Large-Scale Functional Screens

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   ...sgRNA incorporating two MS2 RNA aptamers at the tetraloop and stem-loop 2; 3. The MS2-P65-HSF1 plasmid which...

10. Lighting Up Cell Signaling with Photoswitchable Kinases

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    ...prone to aggregation in cells compared to the tetrameric Dronpa it was derived from. What are photoswitchable...

11. Choosing the B(right)est Fluorescent Protein: Aggregation Tendency

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    ... In corals, the red fluorescent proteins form tetramers. In general, fluorescent proteins have a natural...

12. Plasmids 101: Cre-lox

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    ...orientation, allowing the four Cre proteins to form a tetramer. A double-strand DNA break occurs within the core...

13. CRISPR 101: Anti-CRISPR Proteins Switch Off CRISPR-Cas Systems

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    ...Acr proteins can also be used to prevent dCas9 tethering to DNA. Since these early anti-CRISPR studies,...

14. Antibodies 101: ELISA (Enzyme-linked Immunosorbent Assay)

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    ...reaction of HRP with its substrate, 3,3′,5,5′-Tetramethylbenzidine (TMB). The addition of hydrochloric acid...

15. Split Fluorescent Proteins for Studying Protein-Protein Interactions

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    ...in N-terminal fragment Constant rate of p53 tetramerization in response to DNA damage controls the p53 ...

16. Popular Retroviral Vectors and Their Uses in Scientific Research

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    ...You can clone your gene of interest into this tetracycline inducible, 3rd generation lentiviral vector ...

17. Quickest Way to Deposit Plasmids: The Deposit Spreadsheet

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    ...nourseothricin (clonNat), spectinomycin, streptomycin, tetracycline, combinations of the above, or other. High...

18. Starter guide to induced pluripotent stem cells (iPSCs) part 1:  A renaissance in regenerative medicine

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    ...perspective of stem cell research. Best Pract Res Clin Obstet Gynaecol, 2004. 18(6): p. 827-42. PubMed PMID: ...

19. When is a Monomer not a Monomer? The Top Three Ways Your Favorite Fluorescent Protein Oligomerizes in Cells

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    ...dimerization (i.e. EGFP) [1, 2] or even form obligate tetramers (i.e. DsRed) [3]. This is a problem for fusion...

20. Light Sheet Fluorescence Microscopy

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    ...spinal cords by combining Ultramicroscopy with tetrahydrofuran-based tissue clearing (13,14). A few years ...