...system that is the best fit for the experiment, trying to produce virus of a usable titer, or fine-tuning...beginning an experiment, it is recommended that you try a few different ratios of transfer, envelope, and...

...first (and second) you won’t succeed, but you must try, try again. Now, this does not mean that you should...a different university, perhaps in a different country, choose where you go carefully. Funding agencies...

...Experiments tend to be equally complex with users trying to probe multiple targets in parallel. In these...cross-reactivity. To further limit species cross-reactivity, try to find secondary antibodies that are all derived...Mouse Tissue Sections. In Journal of Histochemistry & Cytochemistry (Vol. 46, Issue 8, pp. 977–983). ...

...currently available, you now face the challenge of trying to figure out what system might work best for your...streamlined approach, you might want to give Gateway a try. This method, developed by Invitrogen and based on...

...started: Determine what type of outcome you are trying to achieve: Do you want to permanently knock out...to activate or repress gene expression? Are you trying to create a single point mutation? Do you want ...

...gondii genes.  2) What genetic modification are you trying to make? Addgene carries CRISPR libraries for gene...activation, repression, and barcoding.  3) Are you trying to target every gene in the genome, or a specific...

...and to get their answers to the questions. Don't try to get all panelists on the phone at once – you won't...knows some of the people in the audience. I always try to meet audience members before a panel discussion...

...to verify plasmids or validating your new clones, try these tips to make the most of your time and sequence... a number of sequence alignment tools available. Try these resources for lists of alternatives to BLAST...databases available are unlikely to have an exact entry for your favorite gene fused to an epitope tag or...

...Meet People at a Scientific Conference New to SfN? Try A Novice's Perspective on Neuroscience - SfN17!  ...

... maybe even picked up a new technique or tool to try in your own work!) If you've got a research story...

...glycerol stock before you begin your plasmid mini-prep. Try not to freeze/thaw your glycerol stock too many times...

... that you cannot perform your duties. Be sure to try out different sizes to know your fit. It is best ...

...confocal microscope. You’re trying to get better images for a paper and she’s trying to get some data for an...

...much easier to go where your audience is than to try to convince your audience to come to you (a TikTok... project like a hot potato or burn yourself out trying to slog through unappealing work in your precious...used in all countries; not all areas of any given country have equal access to high-speed internet. Before... Written works Low Flexible. Low barrier to entry. Ample distribution channels both digitally and ...discouraging to build audience. Need space & higher entry barrier for audience versus passive digital consumption...combine multiple types of media. High barrier to entry (people have to enter a building or exhibit). No...video. Easy to target audiences. Low barrier to entry for both audience and communicators. Well-developed...

...and doing everything, it can be annoying when we try to use them in an experiment and they don't do anything...during replication. To avoid genomic rearrangements, try amplifying the viral vector with bacteria designed...adsorption on target cell membranes." Biophysical chemistry 97.2 (2002): 159-172. PubMed PMID: 12050007. 7...

...molecular biology tool kit. Consider giving it a try next time you’re screening for positive clones! ...the Addgene Blog Learn to do Restriction Cloning Try SLIC cloning Learn about Gibson Assembly Additional...

... writing a review, or if you’re currently stuck trying to write one, hopefully this post will help you... or recent review exists. Once you pick a topic, try to be specific about exactly what aspect of the field...

...Recombines with the entry clone to generate an expression clone   Ready to try out Gateway cloning?...Press). Additional Resources on the Addgene Blog Try Out FastCloning Learn About Methylation and Restriction...to generate entry clones Entry vector attL sites for recombination Used to generate entry clones by TOPO...1: Generate an Entry Clone There are a few different ways to generate our desired entry clone - human ... an attL-containing entry vector.   Figure 4: Method B to create an entry clone: TOPO cloning ... an attL-containing entry vector.   Figure 5: Method C to create an entry clone: Restriction cloning...an attL-containing entry vector.   Pro Tip: Addgene also has ready-made entry clones available for...

...less resources and are particularly useful when trying to screen a limited number of cells like primary... plays a significant role in library efficiency. Try the Dolcetto library! Now also available as a lentiviral...

... and should be designed accordingly. If you are trying activate a gene, you should design a control that...that shows maximal activation. Likewise if you are trying to repress a gene, your control might be a system...