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We narrowed to 580 results for: vit;

Showing: 81 - 100 of 580 results

  2. The Effect of COVID-19 on Liu Lab Plasmid Requests From Addgene Data

    Type
    Blog Post
    ...dip in plasmid request activity around March. A rough estimate shows that activity level in March was around...the effect of COVID-19 on worldwide scientific activity. However, this type of open-ended question is ...plasmid requests from Addgene, as a proxy for global activity in our particular scientific subfields.  Plasmid...of the parameters we infer, since scientific productivity is better interpreted as the rate of scientists.... Values are the inferred percentage of normal activity (the average rate of unique PIs/labs placing orders.../15/20 (the supposed end of lockdown), request activity appears to return to normal. The model fit these... compatible with an infinity-fold reduction in activity due to COVID-19 to zero. Thus, depending on when...

  5. Plasmids 101: The Promoter Region – Let's Go!

    Type
    Blog Post
    ...polymerase. SP6 polymerase has a high processivity. When used for in vitro transcription, the promoter drives...Typically contains a minimal promoter with low basal activity and several tetracycline operators. Transcription... in dicots, less active in monocots, with some activity in animal cells. Ubi General expression mRNA...Description Expression Additional considerations T7 in vitro transcription/ general expression Promoter from ...but requires T7 RNA polymerase. When used for in vitro transcription, the promoter drives either the sense...through varied inducer concentrations.  Sp6 in vitro transcription/ general expression Promoter from ... temperature sensitive cI857 repressor. T3 in vitro transcription/general expression Promoter from T3...

  6. CRISPR Plasmids - Xenopus

    Type
    Collection
    ...NGG) 51306 pUC57-Simple-gRNA backbone T7 BsaI In vitro transcription none, need Cas9 plasmid Chen Do you...

  7. AAV Viral Preps

    Type
    Collection
    ...is used in a wide range of experiments, both in vitro and in vivo. It has low immunogenicity, is easy ...

  8. Starter Guide to induced Pluripotent Stem Cells (iPSCs) Part 2:  Reprogramming and Transdifferentiation

    Type
    Blog Post
    ...Transdifferentiation in vivo or in vitro in vitro Both in vitro and in vivo Epigenetic marks Complete...redifferentiation into the cell type of choice in vitro is known as reprogramming [1]. The process can be...the earliest examples of transdifferentiation in vitro where treatment of mouse fibroblasts with 5-azacytidine...original somatic cells [41]. The in vivo and in vitro methods are similar except that the vectors carrying...reprogramming process as they are propagated in vitro as part of the process. This opens up a wide range...be to dedifferentiate cells from the patient in vitro then correct the damaged gene in the resulting iPSCs...PMID: 22632979. 14. Sasaki, K., et al., Robust In Vitro Induction of Human Germ Cell Fate from Pluripotent...

  11. Brzezinski Lab CRISPR Collection

    Type
    Collection
    ... a unique enhancer and either Ascl1 or Neurog2 activity . Development, 148(12). doi: 10.1242/dev.199399...

  12. Hot Plasmids - March 2019 - Anti-CRISPR, 2in1 Cloning, Fluorescent Voltage Indicators, and Photoswitchable Proteins

    Type
    Blog Post
    ...controlling CRISPR. To bring greater control to Acr activity as a biological tool, Niopek and colleagues turned...have developed a system to control CRISPR-Cas9 activity using blue light (Bubeck et al., 2018). They accomplished... AcrIIA4. In the dark, AcrIIA4 inhibits CRISPR activity.   With the addition of blue light, the terminal...fusion protein inhibitor CASANOVA for “CRISPR-Cas9 activity switching via a novel optogenetic variant of AcrIIA4...2018. PubMed PMID: 30377362. Studying neuronal activity using the fluorescent voltage indicator Archon... Historically, scientists study the electrical activity in the brain by inserting electrodes into the ...of proteins for one that can report electrical activity via imaging. They took the light-sensitive protein...

  13. CRISPR 101: Cytosine and Adenine Base Editors

    Type
    Blog Post
    ...2020). It has increased CBE activity and reduced RNA off-targeting activity compared to ABEmax. In an alternative...to off-target editing based on Cas9 off-target activity.  Find CRISPR base editing plasmids at Addgene...). For each base editor, they observed editing activity with a minimum efficiency of ~50% and confirmed...TadA from ABE7.10 without increasing off-target activity (Richter et al., 2020). This is important because...ribonucleoprotein delivery decrease BE3 off-target activity Komor et al., 2017 BE4 and BE4-Gam; AID, CDA1...evolution of adenine base editors with increased activity and therapeutic application. Nature Biotechnology...efficient cytosine base editor with low off-target activity. Nature Biotechnology, 41(5), 673–685. https:/...

  14. Hot Plasmids May 2018 - Optogenetics, Decaffeination, Biosensors, and Fluorescent Protein Tools

    Type
    Blog Post
    ...detect PKA activity can be generalized to measure phosphatase activity, second messenger activity, and protein-protein...faster assembly and disassembly dynamics, higher sensitivity to light, and better distribution of clusters...biosensors for live-cell imaging of biochemical activity Article contributed by Angela Holmes Jin Zhang... imaging of the dynamic changes in biochemical activity within the cell. Their Fluorescence fLuctuation...Dronpa. The authors tracked protein kinase A (PKA) activity at the plasma membrane in living cells using a...different time points, the authors tracked protein activity while also capturing information about spatial...

  15. SciComm: Taking Science to Elementary and Middle Schools

    Type
    Blog Post
    ... make this STEM activity fun,” but rather “let’s see how we can make this fun activity relate to STEM....their expertise with the kids through some fun activities. If your school doesn’t have a Math & Science...parents from any background, it really helps if activities are run by parents who can answer some of the... a lot of kids, you’re going to want a lot of activities (try for a dozen!). The best approach may not...STEM.” Figure 1 shows the activities we had for my school’s Math & Science Night. There are plenty of places... places to get ideas for science activities. I did some crowdsourcing on Twitter and got lots of suggestions...Steam Powered Family  Biology and Chemistry activities from Quatr.us Foldscopes Steve Spangler Science...

  16. CRISPR Plasmids - Base Edit

    Type
    Collection
    ...CRISPR systems with wiki entries describing enzyme activity, experimental considerations, and more, created...

  17. Tips for CRISPR Gene Editing in Mice

    Type
    Blog Post
    ...your in vivo work is to screen you gRNA in vitro first. In vitro screening is a simple and fast method for...selecting the guide RNA, validating its efficacy in vitro, and using it in mouse embryos to generate gene ...supplied by the Zhang lab. This simple website invites you to input the target region (approximately 500bp... many previously validated gRNAs at Addgene. In vitro screening for the best gRNA Figure 1: Validation...Validated gRNAs to Target Mouse Genes To begin the in vitro screening process, you should first develop primers...in vivo. Find Plasmids for Testing Your gRNAs in vitro at Addgene CRISPR/Cas9 gene editing in mice Now ...  in vitro screening...

  18. Antibody Validation for Flow Cytometry

    Type
    Blog Post
    ...the approaches that can be used to determine selectivity of an antibody for flow cytometry experiments...well as a pragmatic approach to determining the selectivity of antibodies for other sample types and for ...For these reasons, it is essential to test the selectivity of your antibody. But testing is not one size...your experiment. An antibody that demonstrates selectivity for cell surface labeling may become less selective...knockdown to enable robust assessment of antibody selectivity. Additionally, it can be a real challenge to ...protein level at the timepoint studied Off-target activities of the RNAi Correctly interpreting your data...protein expression makes confirmation of antibody selectivity even more challenging. Correlation with RNA or...

  19. How to Design Your gRNA for CRISPR Genome Editing

    Type
    Blog Post
    ...balance maximizing on-target activity while minimizing off-target activity, which sounds obvious but can...100nt window downstream of the TSS gives the most activity. Thus, a given gene will only have a dozen or ...the method of producing the guide (synthetic, in vitro transcription, or lentiviral delivery) can affect...unwise to make conclusions on the basis of the activity of a single gRNA, and thus diversity of gRNAs ...approaches. Avoiding off-target effects The off-target activity of gRNAs is important to consider. While the basic...mismatches that can nevertheless still lead to activity has been established, and can be used to identify... indicates that the consequences of off-target activity, at least for the experimental conditions used...

  20. CasPEDIA: A Functional Classification of Cas Enzymes

    Type
    Blog Post
    ...CasPEDIA feature. Activity features: CasID CasPEDIA will display both the cis and trans activity of each entry...enzyme: Nuclease properties (CasID): Cis/trans activity, PAM requirement, gRNA, and multiplex ability ...strands of its dsDNA target in cis and has no trans activity. AsCas12a, on the other hand, generates staggered...manually enter details to refine your search (trans activity, multiplex ability, gRNA design, etc.). With this... an evolving database, and all researchers are invited to contribute to it! The CasPEDIA Consortium will...update entries as research progresses, but you are invited to reach out to suggest new entries or even new...
Showing: 81 - 100 of 580 results