...silencing complexes (RISCs), which detect dsRNA in the cytoplasm and use it as a guide to complementary RNA ...RNA sequence. Once a compatible sequence is found (typically RNA that the RNAi is designed against), RISC...and will dissociate when gene transcription is complete. However, transcription of thousands of genes ...

...recombination events. The system consists of two components derived from the P1 bacteriophage: the Cre recombinase...recognition site. The P1 bacteriophage uses these components as part of its natural viral life-cycle, and ...and researchers have adapted the components for use in genome manipulation. Cre recombinase, originally...other sites of the same type, but are not cross-compatible. Using different lox site variants allows Cre...

... formation of replication competent lentivirus (RCL). Replication competence can arise from recombination...drive high levels of expression of certain viral components; according the manufacturer these changes result...downstream. When assessing lentiviral titer, don't compare apples to oranges After choosing a packaging system...

...optogenetic or chemogenetic receptors) enables complex control of specifically defined neuronal populations...how newly generated neurons integrate into the complex pre-existing circuit of the brain. Another study...anatomical regions of the brain receive inputs and compute outputs. In general, RABV monosynaptic tracing ...circuitry, and continue to contribute answers to the complex questions of how the brain operates. References...

... is important to fully characterize the compound, or compounds, you choose to ensure reliable results ...In order to understand the complex wiring of the brain and the underpinnings of neurological disease,...pharmacodynamic actions of clozapine-N-oxide, clozapine, and compound 21 in DREADD-based chemogenetics in mice. Scientific...

...’ll share the basic composition of these solutions below. Alternatively, companies including ThermoFisher... tool to identify a protein of interest in a complicated solution like cell lysate. But they can be a ...lost each time you strip a blot, you should not compare the strength of bands from different rounds of ...

...audience’s eye and draw people in. That can be accomplished with an exciting title and attractive figures...in less than a couple of minutes. How does one accomplish all of that? What follows are some tips to help...logical progression of your work, and you can accomplish this by employing a neat and organized format...want to make sure that the color of your font is compatible. Use high-contrast between these colors so that...

...up clear tasks for each member of the team to accomplish so that many elements of the project were able...involved definitely contributed to our ability to complete the project on such a short timescale. Our article...extensive infrastructure of molecular and electronic companion technologies that has transformed the assay’s ...of Zika Virus Using Programmable Biomolecular Components." Cell (2016). PubMed PMID: 27160350. Find Plasmids...

...better at producing a compound of interest, thus allowing them to optimize the compound production process...fluorescent proteins have usurped old methods or complemented them in ways that make them much easier. A special...Although it is possible to make a timer system composed of two separate FPs (one that matures quickly ...

...protein capsid encapsulating the genome is majorly composed of three proteins that can be easily modified ...Delivering gRNA and Cas9 using adenoviral vectors As compared to other viral vectors, AdVs can provide a large...AdV-mediated transduction of gRNA:Cas9 ribonucleoprotein complexes into transformed and non-transformed cells yielded...vector delivery of RNA-guided CRISPR/Cas9 nuclease complexes induces targeted mutagenesis in a diverse array...