Skip to main content

We narrowed to 960 results for: comp

Showing: 491 - 500 of 960 results
  1. Plasmids 101: Gram Negative and Positive Bacteria

    Type
    Blog Post
    ... bacteria is due to differences in cell wall composition. Cell wall structure Gram positive bacteria, ...is added, it interacts with the CV+, creating a complex within the peptidoglycan layer, staining the cell...the peptidoglycan layer, trapping the large dye complex created by the interaction of CV+ and I- in the...type of bacteria it is being used in, and how components of the plasmid should change based on bacteria...
  2. Using CRISPR/Cas9 to Edit Disease Out of the Genome

    Type
    Blog Post
    ... bulk of which you can find below. CRISPR for complex genetic diseases As CRISPR technology becomes increasingly...using it to investigate potential therapeutics for complex genetic diseases. So we’re revisiting this post...look at CRISPR’s potential for use in treating a complex genetic diseases, Alzhiemer’s disease (AD).  Troy...fibrosis (8).   The difference with CRISPR/Cas9 in comparison to other genome editing techniques is its ease...
  3. Creating accessible biology activities in schools with BioBits

    Type
    Blog Post
    ...cellular level. The reason for this is cost and complexity – anyone who works in the lab knows how expensive...expensive tiny amounts of reagents are, how complicated (and again, expensive) the different machines are...kits based on freeze-dried, cell-free (FD-CF) components. Making proteins outside of a cell - cell free..., feel free to check out one of our kits and accompanying curriculum to see if any of the activities fit...
  4. Hot Plasmids - December 2019 - CRISPR pooled library, LipoGlo, CRISPRa, and Voltage Imaging Tools

    Type
    Blog Post
    ... the vascular wall. ApoB, the main structural component of atherogenic lipoproteins, shuttles lipoproteins...Thus, the bEFPs bind the UAS via the dCas9-gRNA complex which allows transcription from the promoter.  ...should serve as both a powerful tool for generating complex regulatory systems and further the study of natural...hypothesizes that this may be due to different lipid compositions of C. elegans membranes, which might beneficially...
  5. Addgene Welcomes John Doench to the Advisory Board

    Type
    Blog Post
    ...Sharp. After a few rotations, Doench decided to complete his PhD work in Sharp’s lab.  Doench says, “what...what I loved about [RNAi], ... it was just this complete black box. The RNAi process had just been discovered...evolved into the Genetic Perturbation Platform and encompasses a variety of technologies to functionally investigate...he says, “In the case of CRISPR, the hype was completely justified… it has been just an incredibly useful...
  6. Finding and Joining Your Dream Lab

    Type
    Blog Post
    ...guide for the quest of a perfect "fit". How competitive is the lab? Some labs have only one author plus...labs around the world. You may prefer a more competitive style to spur you to succeed or you might enjoy...enjoy working closely with others to accomplish your goals. You must understand your own style before making...Finding your dream lab While none of us will feel completely happy at work all of the time, the goal is to...
  7. REPLACR Mutagenesis: Replacing In Vitro Recombination Methods

    Type
    Blog Post
    ...No No Yes Yes Lab Made Competent Cells Required Yes No No No Comparison of REPLACR-mutagenesis with...mutations, large deletions/insertions require complicated, often costly in vitro assembly methods. A new...-mutagenesis also requires making your own electrocompetent cells in which the phage recombination machinery...
  8. Quick Guide to Working with Drosophila Part 3: Genome Engineering in Flies

    Type
    Blog Post
    ...chromosome. Targeted Insertion allows for direct comparisons between lines expressing different variants of...chromosome your transgene has integrated. This can be accomplished with a few matings to balancer chromosome lines...into a plasmid, you can send your plasmid to a company. For a few hundred dollars, they will inject the... your strategy and the services offered by the company, you might have to map into which chromosome the...
  9. Hot Plasmids December 2018 - C. elegans RNAi, TurboID, Detecting Viral Outbreaks, and Protein Binding Specificity Tools

    Type
    Blog Post
    ...to physically set up in the lab, RNAi is often completely ineffective or results in low penetrant phenotypes...membranes, organelles, and other subcellular compartments is via proteomics--the study of the distribution...low-cost, cell-free detection assay that can be completed without special equipment. What’s more, detection...encodes the LacZ𝛼 protein. Once expressed, LacZ𝛼 complexes with LacZ𝜔 and forms an active LacZ tetramer....
  10. Why and how to start studying chromatin (with Addgene's help)

    Type
    Blog Post
    ...being depicted, we can’t forget that those other components are still there in real life.  Certainly referring...interact with each other. Chromatin regulation is complex and influences--directly or indirectly--many other...PIs, articles, genes, and blog posts are not comprehensive, so just because you do not see a name or gene...phosphoinositol-dependent binding of the SWI/SNF-like BAF complex to chromatin after T lymphocyte receptor signaling...
Showing: 491 - 500 of 960 results