Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 1,019 results for: CAL;

Showing: 981 - 1000 of 1019 results

  6. Plan Your Experiment

    Type
    Guide
    ...with most experiments, you first need to have a biological question in mind. Then you can decide how to ...additional sites throughout the genome. These sites are called off-targets. Be aware of off-target sites when ...protein and gRNA, which you generally perform by chemical transfection or electroporation. Expression can...questions: Does the resulting population need to be identical (clonal) or is a mixed population okay? Does the...from a single cell and will result in cells with identical genomes. If your end goal is to establish an edited...Perturb-Seq: Dissecting Molecular Circuits with Scalable Single-Cell RNA Profiling of Pooled Genetic Screens... Kampmann, M., & Weissman, J. S. (2014). Genome-Scale CRISPR-Mediated control of gene repression and activation...

  13. Pipetting Protocol

    Type
    Protocol
    ... using, you will be rotating dials behind the numerical display or the top of the plunger of the pipette... to pipette, it’s important for scientists to calibrate their pipettes to make sure that they are properly...

  15. Plasmids 101: Colony PCR

    Type
    Blog Post
    ...-up Setting up colony PCR reactions is nearly identical to preparing a standard PCR reaction: combine ...

  18. Plasmids 101: Gateway Cloning

    Type
    Blog Post
    ...the attB, P, L and R sites that recombine very specifically and directionally: attB1 sites react only with...

  20. Coomassie Purity Stain of Recombinant Antibodies

    Type
    Protocol
    ...trendline for the standards. Google Sheets can calculate this for you as follows: In the Chart Editor ,...based on your sample’s HC + LC area. Example calculation: If the sample HC + LC Area is 18179.442 and ...
Showing: 981 - 1000 of 1019 results