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We narrowed to 1,076 results for: TOR

Showing: 981 - 1000 of 1076 results

  1. Mammalian RNAi Tools

    Type
    Collection
    ...shRNAs are often delivered using viral vectors. Lentiviral vectors are a preferred method for long-term ...Plasmid Vector Type PI Publication Additional Resources Addgene Resources pLKO.1 cloning vector protocol...long-term gene silencing. Once the viral vector is taken up by a host, the viral genome is transcribed, producing.... shRNA Plasmids Browse our selection of empty vectors for cloning in shRNAs. Most plasmids are in a lentiviral...Lentiviral packaging plasmids Web Resources Trono Lab Lentivectors Toolbox Optimized shRNA design in pSico with...

  2. CRISPR Plasmids and Resources

    Type
    Collection
    ...Empty gRNA Vectors Select an empty vector for your experiment based on a variety of factors. gRNAs gRNA...for you to download. CRISPR Resources : Browse depositor protocols, find software for gRNA design and deep...discover links to CRISPR forums, and more. CRISPR History : Learn how CRISPR was modified from a bacterial...information into a specific DNA site. Base Edit Base editors precisely edit DNA without promoting DSB formation...Transcriptional Regulation Activate dCas9 fused to an activator peptide activates gene expression. Interfere dCas9...

  3. AAVED

    Type
    Collection
    ...meeting. The goal of this year’s workshop is to brainstorm practical strategies for using combinations of... Institute Katherine Matho Cold Spring Harbor Laboratory Gowan Tervo Janelia Research Campus Sarada Viswanathan...Viswanathan Janelia Research Campus Kenton Woodard Penn Vector Core Melina Fan, Karen Guerin, and Leila Haery ...Regulation Ben Deverman 2:00 PM Cre-DOG and Combinatorial Strategies Connie Cepko 2:45 PM Break 3:00 PM...using this techonology in the lab (main issues/factors that researchers should consider when planning ...Institute Katherine Matho, Cold Spring Harbor Laboratory Gowan Tervo and Sarada Viswanathan, Janelia Research... Research Campus Kenton Woodard, Penn Vector Core Intended Outcome of Meeting The outcome of this meeting...

  4. Allen Institute for Cell Science Plasmid Collection

    Type
    Collection
    ...mEGFP AICSDP-60 mEGFP Transcription factor SOX-2 Transcription Factor 124607 ACTN2-mEGFP AICSDP-63 mEGFP...repeat-binding factor 2 (TRF2) Telomeres 168799 CTCF-mEGFP AICSDP-144 mEGFP CCCTC-binding factor (CTCF) CTCF-binding... Depositor Collections Allen Institute for Cell Science Plasmid...UBTF-HaloTag AICSDP-80 HaloTag Nucleolar transcription factor UBF Nucleolus (fibrillar center) 133964 SON-mEGFP...TFAM-mEGFP AICSDP-72 mEGFP Mitochondrial transcription factor A (TFAM) Mitochondrial nucleoids 164499 EZH2-mEGFP...

  5. Synthetic Biology - Cloning and Genomic Tools

    Type
    Collection
    ...plasmids available from this depositor's lab. Plasmid Description Gene/Insert Vector Type PI Publication Back...available plasmids from this depositor's lab. Plasmid Description Vector Type Selectable Marker PI Publication...Construction Integration Recombination Shuttle Vectors Plasmids for Cloning and Genomic Tools Search the...Back to Top Shuttle Vectors Search the table by keyword or sort by the table headings. Click on the publication...

  6. NETRF

    Type
    Collection
    ...from the NETRF-funded investigators listed below. Depositing Principal Investigators (PIs) PI Institution...Addgene Depositors that have received funding from the Neuroendocrine Tumor Research Foundation Depositor...Shivdasani Dana-Farber Cancer Institute Epigenetic Regulators of Intestinal Endocrine Cells and Carcinoid Tumors...Tumors Qiao Zhou Harvard University Epigenetic Regulators of Intestinal Endocrine Cells and Carcinoid Tumors...

  7. Botman-Teusink Yeast FP Collection

    Type
    Collection
    ... Depositor Collections Botman-Teusink Yeast FP Collection...Botman et al., 2019 . FP Tagging Yeast tagging vectors to create fusions of proteins of interest with ...yeast codon-optimized fluorescent proteins. These vectors are based on the pFA6a-link plasmid (Sheff et al...overexpression of codon-optimized FPs in yeast. Vectors are based on the pDRF1 plasmid (Loqué et al., 2007...blue, green, and red fluorescent protein tagging vectors for S. cerevisiae. PLoS One. 2013 Jul 2;8(7):e67902...Zhou Y, Nielsen LK. Dual gene expression cassette vectors with antibiotic selection markers for engineering...

  8. Resolute Plasmid Collection

    Type
    Collection
    ... SLCs in Gateway-compatible pENTR vectors (pDONR221). These vectors allow a fast and efficient sub-cloning... Depositor Collections Resolute RESOLUTE Plasmid Collection...framework that provides biological context. Assays monitoring impedance, fluorescence, mass/charge properties... into a wide range of Gateway-compatible pDEST vectors. ID Plasmid Gene/Insert Industry PI Pooled Libraries...the Resolute Partnership using their RESOLUTE repository feedback form . If you have further questions...

  9. Recombinases AAV Preps

    Type
    Collection
    ...plasmids in the repository. Browse our AAV collection of site specific recombinases.... Viral Vector Packaging ...Packaging Service AAV Recombinases Viral Vector Packaging Service: Recombinases Cre AAV Dre AAV Flpo AAV Light-Inducible...Karpova and Schaffer labs. See the Retrograde AAV Inventory Page . Don’t See What You’re Looking For? Our ... eligible AAV plasmids and we’ll make the viral vector prep you need. Or fill out our form to let us know...format. Please note this does not guarantee viral vector packaging service, but lets us know what viruses...

  10. New England Biolabs Cell-Imaging Plasmid Collection

    Type
    Collection
    ... Depositor Collections New England Biolabs Cell-Imaging Plasmid...Plasmid Substrate Feature 101137 pSNAP-tag (T7) Vector SNAP-tag Empty backbone for bacterial expression...expression under T7 control 101135 pSNAP-tag (m) Vector SNAP-tag Empty backbone for stable and transient mammalian... cell surface localization 101136 pCLIP-tag (m) Vector CLIP-tag Empty backbone for stable and transient...for cell surface localization 101127 pMCP-tag (m) Vector ACP/MCP-tag Empty backbone for transient mammalian...mammalian expression 101126 pACP-tag (m)- 2 Vector ACP/MCP-tag Empty backbone for stable and transient mammalian...

  11. Distribution to Industry

    Type
    Collection
    ...signed by an authorized signatory at the recipient institution. An authorized signatory is someone who can ...also adding additional filters such as expression, vector type, species, and more. Browse Browse the table...Golden Gate Assembly Standards Kit Cloning Eric Cantor Kit for using comprehensive end-joining ligation...Libraries CRISPR Thorsten Stiewe Use these donor vector libraries for CRISPR/Cas9-mediated saturation genome...entity is qualified to receive materials from our repository. All purchases are subject to Addgene’s Terms...

  12. Zinc Finger Consortium: Zinc Finger Arrays

    Type
    Collection
    ... within that gene. To construct ZFN expression vectors, DNA encoding zinc finger arrays should be cloned...plasmids also available through Addgene--the specific vector to use depends on the length of the spacer sequence...between the half-sites (see Nuclease Expression Vectors for more details). IMPORTANT NOTE: All zinc finger...tCCCATCCTAtcgttGCATGAGCTc mdka (midkine-related growth factor) OZ527 and OZ528 OPEN OPEN cACCCTCCTCgtggtGCTGGTGGCc...aAGCTTCCTCttcattgGCTGACTGCa ldr (low density lipoprotein receptor) OZ539 and OZ540 OPEN OPEN tTGCATCCACggcagTCGGCAGTGc...OPEN OPEN gAGCTCCGTCtcgctgGCGGCCGAAg histamine receptor H3 (hrh3) OZ549 and OZ550 OPEN OPEN gCGCCACGGAcaataaaGAGGACTGCa...

  13. Worm Expression Resources

    Type
    Collection
    ...General Tools Fire Lab C. elegans Vector Kit - Andrew Fire Lab. A set of vectors for C. elegans research, including...plasmid tools for the community that aren't in the repository? Help us widen our resources for the community...plasmids that streamlines the production of targeting vectors for tag insertion using CRISPR/Cas9 and the selection... approach has also been developed by the Fire laboratory and described in Efficient Marker-Free Recovery...including lacZ and GFP fusion vectors. C. elegans optimized fluorophores - Andrew Fire Lab. Available ...

  14. Arf GTPase Family

    Type
    Collection
    ...References The ARF (ADP-ribosylation factor) family of regulatory GTPases have long been characterized...exchange factors), and downstream (by GAPs, GTPase activating proteins, which also serve effector roles)...ARF family plasmids. ARF family members include regulatory GTPases, GEFs, and GAPs. Plasmid..., 2016). Though best known for their roles as regulators of membrane traffic, they also play critical ...

  15. Synthetic Biology - Networks and Gene Regulation

    Type
    Collection
    ...Examples Include: promoters and terminators repressors and activators logic gates Networks and Gene Regulation...plasmids available from this depositor's lab. Plasmid Gene/Insert Vector Type Promoter PI Publication ...includes both naturally ocurring and synthetic regulatory elements, pre-assembled genetic circuits, and...

  16. TALENs for Endogenous Zebrafish Genes

    Type
    Collection
    ...TGGATGGCGAGAACAGGGcggtggagtataacgcTGTGGGCCTGACGAAGGA Complement Factor H TAL3230 & TAL3231 TGATTACATGTGAACCTCcatcacgtgaccagcatgTGTCCACCTCCTTTTGGA...TAAACGCCCTGATCTTCAgcgccgacggcctgcagcAGAGAGACAAGATGCGCA inhibitor of DNA binding 3 TAL3466 & TAL3467 TCAACATTGGATATCTATagagctttacagctagTTTATCCCAGTGTCCAGA...TCATGGTGCAGCCGTTACtggacagccgcgttccCTACGGACGCCTCCAGCA Purinergic receptor Y2 TAL3348 & TAL3349 TTCAACGCATCAGTGAATgttcaaaaccacagttCTGCACACAGCTGCAGCA...TCTTTTTGTGCTGCGCCAcccagcccataatcgaGTCTTCTACAGGCATGAA retinitis pigmentosa GTPase regulator b TAL3528 & TAL3529 TGGAGAAACAGAAGATGAaatccccgagtccggaGCTGTCTTCACTTTTGGA...TGGAGAAACAGAAGATGAaatccccgagtccggaGCTGTCTTCACTTTTGGA retinitis pigmentosa GTPase regulator b ORF15 TAL3530 & TAL3531 TAAGAGGGCGGAGCTTTTatctcgcaaagctctgCCCACTGAGTTACTTAGA...TGACTGGGAGGAAGATCAgagtcccgttgtgtctTGCAGCTCAGGATTTGGA Vascular-endothelial growth-factor-aa (vegfaa) TAL3200 & TAL3201 TCTCGCTTTGCTTCCTCCtcagtgattcccttcaTGGATGTGTATAAAAAGA...

  17. CRISPR Plasmids - Activate Gene Expression

    Type
    Collection
    ...gRNA sequence to direct the dCas9-activator to promoter or regulatory regions of your gene of interest....-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian Bacteria Drosophila ...Catalytically dead dCas9 fused to a transcriptional activator peptide can increase transcription of a specific...separate gRNA expression plasmid to target the dCas9-activator to your specific locus. Want more information ...

  18. CRISPR Plasmids - Zebrafish

    Type
    Collection
    ...Plasmid Gene/Insert Vector Type Promoter PI Publication Empty gRNA Expression Vectors Select a gRNA expression...-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian Bacteria Drosophila ...expression plasmid based on factors such as selectable marker or cloning method. When using CRISPR, you...both the gRNA and Cas protein act as all-in-one vectors, but their function is often limited to a single...

  19. Brzezinski Lab CRISPR Collection

    Type
    Collection
    ...plasmids to study the regulatory networks involved in retinal development.... Depositor Collections Brzezinski...mouse retina. To aide in their studies of the regulatory networks involved in retinal development the ...Prdm1 and Vsx2 enhancers in the retina alters photoreceptor and bipolar cell fate specification, yet differs...

  20. Adeno-associated virus (AAV) Plasmids

    Type
    Collection
    ...Blog: Plasmids 101-Viral Vector Elements Blog: AAV use in CRISPR Viral Vectors 101 eBook Packaging Plasmids...Pseudotyping Want to save time? Check out our Viral Vector Packaging Service and learn about our options for...RepCap Plasmids for Serotypes Available as a Viral Vector Packaging Service at Addgene ID Plasmid Capsid ...species, gene name, and more. ID Plasmid Gene/Insert Vector Type Backbone Mutations Tags PI Do you have suggestions...
Showing: 981 - 1000 of 1076 results