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Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 1,046 results for: fer

Showing: 981 - 1000 of 1046 results

  9. Tips for Technical Support Calls

    Type
    Blog Post
    ...successfully used said plasmids. We provide these references as our way of saying “Hey look! This stuff works...

  15. Bioinformatics at Addgene

    Type
    Blog Post
    ... address the need to accelerate science using different approaches. We were also encouraged to see that...

  18. Western Blot

    Type
    Protocol
    ... tank. Pro-Tip Different SDS-PAGE gel tanks will have different gel orientations. Refer to the instructions...protocol uses a dry transfer device but can be adapted for wet and semi-dry transfer approaches. Last Update...80 °C freezer Reagents 1X PBS Lysis buffer e.g., RIPA lysis buffer Microcentrifuge tubes BCA Assay, Thermo...β-mercaptoethanol 4X protein loading buffer Precast SDS-PAGE gel SDS-PAGE running buffer Prestained protein ladder...appropriate volume of cold lysis buffer. Pro-Tips The volume of lysis buffer will vary depending on the size...lysis buffer will vary depending on the cellular location of the protein of interest. RIPA buffer is suitable...β-mercaptoethanol to the 4X protein loading buffer. Dilute 4X protein loading buffer in the sample to 1X . Boil the...

  19. Affinity Purification of Recombinant Antibodies with Protein A or Protein G

    Type
    Protocol
    .../G binding buffer (20 mM sodium phosphate buffer, pH 7.0) 20 mL 1M sodium phosphate buffer 980 mL of deionized...use. Warm the affinity column, binding buffer, and elution buffer to room temperature (RT) before use. ... pour off the ethanol storage buffer. Tightly attach LabMate PD10 Buffer Reservoirs to the top of the ...to Option 2 with a buffer exchange/concentration using an Amicon 30 kDa MWCO buffer exchange column. (...may need to be adjusted to accommodate slight differences between products. Addgene does not endorse or...Timeline Day 1: Purify antibody Day 2 or later: Buffer exchange Equipment Class II, Type A2 Biological...Filter, 0.2 µm (luer-lock), VWR 431229 IgG Elution Buffer, Thermo Fisher 21028 Protein G GraviTrap columns...

  20. Protocol - How to Run an Agarose Gel

    Type
    Protocol
    ...there is very little difference between the two. Note: Make sure to use the same buffer as the one in the...the gel box (do not mix different buffers and do not use water). Microwave for 1-3 min until the agarose...the running buffer when you run the gel. If you do not add EtBr to the gel and running buffer, you will ... Add loading buffer to each of your DNA samples and mix well. Use 5 µl of loading buffer per 25 µl of ...bubbles or buffer from entering the tip. Place the very top of the tip of the pipette into the buffer just ...the gel and buffer, place the gel into a container filled with 100 mL of TAE running buffer and 5 μL of...bromide (stock concentration of 10 mg/mL) Gel loading buffer, New England Biolabs B7022S Procedure Pouring a...
Showing: 981 - 1000 of 1046 results