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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 1,010 results for: RING;

Showing: 501 - 510 of 1010 results

  1. Plasmids 101: Modular Cloning

    Type
    Blog Post
    ...Most things in biology are not so simple, and engineering complex cellular behaviors requires making complex...output, etc., which can support a larger cellular engineering goal, several of which are described below. Multigene...assembling an array of targeting constructs and delivering them to these organisms via another optimized... an array of distinct goals within metabolic engineering, synthetic product production, and gene editing...

  2. CRISPR in the Clinic

    Type
    Blog Post
    ...involve editing healthy donor-derived cells and delivering them to the patient.   Current CRISPR Therapies... preliminary results (Dimitri et al). CRISPR engineering of CAR-T cells is particularly promising because...Early-stage trials showed a high success rate in clearing misfolded protein levels as measured in the blood...2013). Dimitri, A., Herbst, F. & Fraietta, J.A. Engineering the next-generation of CAR T-cells with CRISPR-Cas9...

  3. Adapting Toehold Switches to Detect Zika Virus

    Type
    Blog Post
    ...risk for the project by initially designing and ordering several dozen versions of the toehold switches...successful sensors to draw on as our assays grew more stringent, and it avoided any days of lost productivity ... virus genome with Addgene to facilitate rapid sharing of these tools with the Zika virus research community...product development, the regulatory process, and manufacturing so that we can get the diagnostics into the ...

  4. Cpf1 Update: Comparison to Cas9 and NgAgo

    Type
    Blog Post
    ...shortcomings. Cpf1’s preferred PAM is 5’-TTN, differing from that of Cas9 (3’-NGG) in both distribution...clinical applications require HDR over NHEJ, so comparing HDR efficiency across CRISPR nucleases and NgAgo...insertion mediated by Cpf1-generated overhangs. Exploring the efficiency of these two processes will help... Mary Gearing...

  5. Popular Retroviral Vectors and Their Uses in Scientific Research

    Type
    Blog Post
    ...Therefore they make excellent tools for genetic engineering. There are several different viral vectors to... and Adeno-associated virus (AAV). If you are wondering which virus fits you experiments best have a look...retroviral vectors can regulate gene expression by delivering shRNA or miRNA. The ever expanding CRISPR field...our CRISPR reagents. Please check www.genome-engineering.org for the most up-to-date information. Check ...

  6. Meet an Addgenie: Insights into Grad School, Career Paths, and More

    Type
    Blog Post
    ...and a Spanish minor, it’s time for me to start considering my future path. Talking to Addgenies has helped...it solidified some concepts that are heard often during undergrad. Lauren Flynn is the Addgenie behind... publish their research and are not so keen on sharing their results in fear of getting scooped, she said...her focus is finance, it is just as important to bring love and fun to the office. Main takeaways from ...

  7. Reaching out to China: Canton Nucleic Acids Forum (CNAF) 2015

    Type
    Blog Post
    ...lab is also exploring the use of cas9 proteins from different species for genome engineering. The final...will lead to advances in human health. It was inspiring to hear how the genome project has already had...complicated conformation “not like DNA which has a boring structure.” That certainly summed up this conference—nucleic...

  8. Experimenting in an Open Source lab: from CRISPR to Cats

    Type
    Blog Post
    ...function as centres for peer learning and knowledge sharing, in the form of workshops, presentations, and lectures...looking at how to re-use it as a platform for monitoring homemade microfluidic chips using the fantastic...members and the public cheap access to personal manufacturing tools – a 3D printer, a laser cutter, woodworking... and an instructor at Biocurious.org, Derek is bringing cutting edge technologies to Victoria, BC. Follow...

  9. The Scientific Conference Poster Session: Tips for Success

    Type
    Blog Post
    ...re a presentation veteran, but have difficulty answering questions. Or perhaps you’re simply attending,...snacks to keep you energized throughout the session, bring extra clips to hold up your poster, and pack a few...seems captivated. Expertly navigate questions Answering questions is arguably the most difficult part ...very interested in his/her work, and would enjoy hearing the highlights so that you can appreciate the impact...

  10. Transferable Skills Guide: Managing a Team

    Type
    Blog Post
    ...transferable skills while working in the lab and by offering advice on areas where you might need some help...mine you didn’t think much about management skills during your scientific training. I never thought about...encourage questions. Unless you’re delegating a recurring task to a seasoned report, there should be questions...cases reports are not aware of issues until you bring them up. I wouldn’t go as far as saying that your...
Showing: 501 - 510 of 1010 results