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We narrowed to 911 results for: TRO

Showing: 501 - 510 of 911 results
  1. Improving Transduction Efficiency with Exosome AAVs

    Type
    Blog Post
    ...transduction profiles in different AAV serotypes both in vitro and in vivo when compared to the conventional AAVs...target less accessible cells, such as brain tumor stromal cells in a mouse model of glioblastoma (Volak et...vector-mediated genetic modification of brain tumor stromal cells after intravenous delivery." Journal of neuro-oncology...
  2. The Crispr Babies Saga Shows the Need for Action, Not More Delays

    Type
    Blog Post
    ...independently verified, the watching world erupted in controversy. Briefly, there are two overlapping, valid critiques...within their respective mandates, to tackle a controversial and ethically fraught rapidly emerging technology...good research practice: computer simulations, in vitro research, animal models, and several stages of human...
  3. What Good Citizenship Can Do for Reproducibility in Science

    Type
    Blog Post
    ...reproduce someone else’s result. Then, they hit trouble. The experiment won’t work. Even if it does, they...to share with other researchers to help them troubleshoot and understand. All of this communication and... of incentives. Right now, researchers are not strongly rewarded for being transparent in their communication...
  4. Plasmids 101: Mammalian Vectors

    Type
    Blog Post
    ...components, it may be useful to describe the means of introducing genetic material (such as plasmids) into mammalian... comparable to bacterial transformation (the introduction of DNA into bacterial cells); however, the techniques...plasmid to be expressed transiently. Since the DNA introduced in the transfection process is not integrated...
  5. The Many Reasons for Irreproducible Research- and a Vaccine to Eradicate It

    Type
    Blog Post
    ...growth rates will be higher in treatment versus control, and you think there are four important times to...preregistration, but usually with a fully fleshed out introduction section) to one of 222 participating journals..., they will place special emphasis on positive controls and good sample sizes, concerns that are too easy...
  6. How to Make Friends and Meet People at a Scientific Conference

    Type
    Blog Post
    ... give you theirs! Think about how you want to introduce yourself and what you do. You can probably use... at a conference, but start with a more general intro until you know if the person you are talking to ...people in sessions by sitting next to someone and introducing yourself. Put your phone away! This is a natural...
  7. Plasmids 101: Inducible Promoters

    Type
    Blog Post
    ...Promoters control the binding of RNA polymerase and transcription factors. Since the promoter region ...promoters can be regulated by positive or negative control. Positive inducible In the OFF state, the promoter...system, the activator rtTA (reverse tetracycline-controlled transactivator) is normally inactive and cannot...
  8. A Novice's Perspective on Neuroscience - SfN2017

    Type
    Blog Post
    ... by stories. In retrospect, I should have expected this given that the brain controls everything we do... at Addgene.org Addgene's Viral Service Find Retrograde AAV Browse Optogenetics Plasmids ...
  9. GCE4All: Making Genetic Code Expansion Accessible

    Type
    Blog Post
    ...acid in the media and the tRNAs and synthetase introduced to the bacteria using plasmids. Rapid expansion...plasmid. Third, a second plasmid will be needed to introduce the gene of interest containing the chosen codon...researchers, from selective protein ligation through introducing ncAAs with protein modifications of interest ...
  10. OCaMP: A New Calcium Indicator for Neural Imaging

    Type
    Blog Post
    ...which is more than just the stuff that makes bones strong; it plays critical roles in nerve and muscle function...fluorescent tools are used simultaneously to monitor or control different cell types or signals in the brain. For...Simultaneous fluorescence (upper trace) and electrophysiological recordings (lower trace) using OCaMP expressed...
Showing: 501 - 510 of 911 results