We narrowed to 1,073 results for: TOR

... Get Management Advice from Addgene Executive Director Joanne Kamens FInd Tips on Picking a Research ...

... to modify the desired genomic locus.   Some historical notes CRISPR arrays in bacterial genomes consist...

...poring over my grandparents’ medical textbooks by torchlight under the covers. In high school, I went to all...

...radio reporter challenge, and code.org has coding tutorials for all ages. Organize your data and prepare ...

...turn genes on or off. Gal4 is a transcriptional activator that binds to UAS enhancer sequences found in ...

...to small fluorescence changes of the donor and acceptor after proteolytic cleavage. Secondly, fluorescence...

...these strains should not be used for long term storage. Additional Resources Review of restriction modification...

....org  View Addgene's collection of empty gRNA vectors View Addgene's CRISPR resources Request ready-to-use...

...secondary antibodies are commonly used in research laboratories to detect the different subclasses, but there...

...excitation wavelength, available emission filters, and detector sensitivity) and the biological system (temperature...

... any other required non-CRISPR-associated host factors required for CRISPR processing. For each enzyme...

... in science brings stronger networking across laboratory and disciplinary boundaries and can allow for...

...Purification Protocol Video rAbs in the Addgene repository...

...You never know when you will meet a potential collaborator Some of these people you meet will review your...

...sites. Often, the size of the plasmid insert and vector backbone are known and thus this technique can ...Restriction Digest If you clone an insert into a vector by a single restriction enzyme, you will need to...which orientation the insert is ligated into the vector backbone and therefore the only thing left to do...

... you can start by paying attention to how your mentors reach solutions and ask them to explain their logic...

...Fishman JB, Berg EA (2019) Antibody Purification and Storage. Cold Spring Harb Protoc 2019:pdb.top099101. https...

...affinity purification of human insulin-like growth factor I from culture medium of E.coli. Bio/Technology...

...secondary antibodies you already have in your refrigerator will not recognize a Fab fragment. In order ...

...independently of any other accessory proteins or co-factors, thus allowing for broad applications in a variety...