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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 1,010 results for: RING;

Showing: 511 - 520 of 1010 results

  1. Transferable Skills Guide: Managing a Team

    Type
    Blog Post
    ...transferable skills while working in the lab and by offering advice on areas where you might need some help...mine you didn’t think much about management skills during your scientific training. I never thought about...encourage questions. Unless you’re delegating a recurring task to a seasoned report, there should be questions...cases reports are not aware of issues until you bring them up. I wouldn’t go as far as saying that your...

  2. Of Myc and Men

    Type
    Blog Post
    ...not recognize the mouse or chicken homologs. By comparing the homologous sequences in these species, they...making the antibody more amenable to protein engineering. As a result, you can now find Myc1-9E10 in a...published a paper showing that the amino acids neighboring the Myc epitope influence 9E10’s affinity for...highly variable epitope recognition dependent on neighboring sequence context. Sci Signal 13. https://doi....

  3. The Scientific Conference Poster Session: Tips for Success

    Type
    Blog Post
    ...re a presentation veteran, but have difficulty answering questions. Or perhaps you’re simply attending,...snacks to keep you energized throughout the session, bring extra clips to hold up your poster, and pack a few...seems captivated. Expertly navigate questions Answering questions is arguably the most difficult part ...very interested in his/her work, and would enjoy hearing the highlights so that you can appreciate the impact...

  4. Deep Dive: Fixing and Permeabilizing for Immunofluorescence

    Type
    Blog Post
    ...steps and outlined the considerations to be made during each step. Now that you know the basic principles...harsh - lipids and soluble proteins can be lost during fixation with these chemicals, which impacts sample...and attention.   * There are other methods for recovering epitope availability following cross-linking ...Immunofluorescence Staining. Methods in Molecular Biology. Springer New York, pp 299–311. https://doi.org/10.1007/...

  5. Plasmids 101: Choosing an Antibiotic Resistance Gene

    Type
    Blog Post
    ...plasmids, or express a gene contained in a plasmid. During the propagation and/or transformation process, ...never receive a copy of the plasmid or will lose it during division. These non-plasmid-containing bacteria...while amp only affects the cell wall synthesis step during cell division. This is nifty because the recovery...it will induce mutations in the host/plasmid DNA during selection if it’s not completely bound up. While...

  6. CRISPR 101: Making a Knock-In Cell Line

    Type
    Blog Post
    ...find out! Homology-directed repair and genome engineering The easiest way to make a knock-in cell line ...built-in repair pathway cells already have for repairing DNA double strand breaks – (HR). HR is the dominant..., H., Ren, S., Yu, S., Pan, H., et al. Methods favoring homology-directed repair choice in response to.... A. Enhanced homology-directed human genome engineering by controlled timing of CRISPR/Cas9 delivery....

  7. Minigenomes - a Safe Way to Study Dangerous Viruses Like the Ebola Virus

    Type
    Blog Post
    ...minigenome system Similar to other RNA viruses, EBOV brings its own RNA-dependent RNA polymerase into the infected...partially control expression levels of each gene by altering the amount transfected (see Technical Tips). Minigenome...changing the minigenome cis-acting sequences and monitoring effects on genome replication and transcription...strand RNA viruses: The power of reverse genetics. Springer Berlin Heidelberg, 2004. 1-41. PubMed PMID:15298166...

  8. Tips for Screening with Yeast Two Hybrid Systems

    Type
    Blog Post
    ...eukaryotic transcriptional activation and cell biology. During this period, proteins were successfully expressed... own, but, if the two POI’s interact, this will bring the POI-TA fusion (commonly referred to as the “.... Lowered expression levels will increase the stringency of your binding. And most importantly: Independently...Ptashne, M. A eukaryotic transcriptional activator bearing the DNA specificity of a prokaryotic repressor....

  9. Fluorescent Proteins 101: Green Fluorescent Protein (GFP)

    Type
    Blog Post
    ...activity – it only requires the presence of oxygen during maturation. This meant that the protein could be...study of dynamic, physiological processes. Re-engineering GFP to increase its range of color and application...its sequence was elucidated, scientists began engineering new versions of GFP through mutagenesis in order...us how! And come back next week when we'll be featuring a guest blog post from Gal Haimovich of greenfluorescentblog...

  10. Using AAV for Neuronal Tracing

    Type
    Blog Post
    ...neuronal loop which is activated and strengthened during memory formation and memory retrieval. In neuronal...recordings. The origins of these methods are naturally occurring viruses that infect, persist, and migrate within...modified and used as tools for tracing. With some engineering, RABV and HSV, but also others including pseudorabies...plasmids at Addgene). AAV vectors: A platform for delivering neuronal tracers AAV vectors have been widely...AAV1 – 9, with most of these serotypes capable of entering neurons (see table 1)(Choi et al., 2005; Taymans...
Showing: 511 - 520 of 1010 results